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The map label for this gene is galE [C]

Identifier: 108797402

GI number: 108797402

Start: 467328

End: 468728

Strand: Direct

Name: galE [C]

Synonym: Mmcs_0422

Alternate gene names: 108797402

Gene position: 467328-468728 (Clockwise)

Preceding gene: 108797396

Following gene: 108797403

Centisome position: 8.19

GC content: 70.45

Gene sequence:

>1401_bases
ATGACAGCAGAGCCTTCGCGAATAGTGGTGACCGGGGCGTCGGGCAATGTGGGAACCGGCGTGCTGCGCGCGCTGGCGTC
CCAGCTGCCCGACGCCGAGGTCGTGGGAGTGTGCCGGCGGCCGCCCACAGACGGTGAGCCCTACGAGCGGGTGCGCTGGC
ACACCGTCGACCTGTCCGCGCCGAGCGCCGTCGCGGACCTGGAACCCGCTCTGCGAGGCGCGGACGTCGTCATCCATCTG
GCGTTGGCCGTCCATCCGGTGCGCGACGAGCACTACCTTTACCGCGCGAATGTGCTTGGCACACAAGCACTCCTGAAGGC
CATGACGGCGGCCGGGGTGAGGCAGCTGGTCTACGCGTCCAGTCTTGGCATCTACGCGCCCGGATCCGGGCCGCCGGTCA
CCGAGGATTGGCCCACGACCGGCCAGACCACCTCGGTCTACAGCCGGCACAAGGTGATGGTGGAGCAGGTCCTGGACGAG
TTCGAGCTCGACCACCCCGAGGTCACGGTCTCGCGGTTCCGCCCGACCGTCGTGGTGCAGCGCGAAGCCGCGTGGCTGAT
CAAATCGTTGTACTTGGGTCCCTTGGTGCCACGGTCGGTGCTGGAACTCCTCCGTCGACGGGAGCTCCCCGTCCTTCCGG
TGCCTGCGCCGCTGGCACTGCAGTTCGTCCACGCCGACGACGTCGGCGATGCAGTGGTCCGGATGGTCACGCAGCAGGTT
CGCGGATCGTTCAACATCGCCGCCGATGTGCTGGACACGGCGGCGCTCGCCGACCTCGTCGGGGCACGCCCGGTCAGCGT
TCCCCCGCAGGCGGTGCGCACGCTCATCGCGGCCCTGAGTGCTGTCCGGGTCGTGGCGCTGACCCCGGGGTGGTACGACG
TGGCGTTCAAAACCCCGCTGATGGACACCTCCAAGGCGCGCCGGACGTTGGGCTGGGAGCCGGCGCGCTCCTCGGCCGAG
AGCGCGCGGGAGTTGATCGAGGGCCTCGCCGACGGGGCAGTGGGCACCAGCGCCGCGATGGGGTGGCGGCTGCGGCCCCG
CAGGGACGTCCGCGCCGCGGTCGATCGTGCGCATGACGTCACCCTGGTGTCATGGGGCGCGCTGGCGCTGCTGCGCGCCG
CCGGGATGCGCCGCGCCCGCGTCGTGGATGCCGTGGTGGTGGCGTCGAACCTGGCCACCGGTACTCCGATGGCGCTGGAC
CGGATATTGGAGCGGCGCGCCGATCCGGTCGCACTGCTCGCGCCGGTGGCTGTCCTGGCTGCCCTCGGGGCCACGCTGTG
CGGTGGCTGGCCCGCTGTTGCCGTCACCGGCGCCTTGCAGGTGCTCCGCATGTCCGAACGCAATCAACGAAAAGCGAAAC
TCGTTAGCAACGCCGAGGTTCCGGCAGGCTCCCGGGGGTAA

Upstream 100 bases:

>100_bases
CGAGCGTGCGTTCATCGTGGCGCCGATTACCGACGGCGCCCGCCGAGGTTTGGTGGACCTCGCCACGGGTAAGCGGCGTC
ATCCGACGAGAACGGACGCC

Downstream 100 bases:

>100_bases
TGCCCCACGATGGATGAATCTGCGGCAACACCCGAAGTGGTCGTGGTGACGGGTGCCTCCAGCGGCATCGGCGAGGAGAC
GGCCCTTCGCTATGCGGGGC

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: Nucleoside-Diphosphate-Sugar Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; NAD Dependent Epimerase/Dehydratase Family Protein; Oxidoreductase; UDP-Glucose 4-Epimerase; Nucleotide-Sugar Epimerase

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTAEPSRIVVTGASGNVGTGVLRALASQLPDAEVVGVCRRPPTDGEPYERVRWHTVDLSAPSAVADLEPALRGADVVIHL
ALAVHPVRDEHYLYRANVLGTQALLKAMTAAGVRQLVYASSLGIYAPGSGPPVTEDWPTTGQTTSVYSRHKVMVEQVLDE
FELDHPEVTVSRFRPTVVVQREAAWLIKSLYLGPLVPRSVLELLRRRELPVLPVPAPLALQFVHADDVGDAVVRMVTQQV
RGSFNIAADVLDTAALADLVGARPVSVPPQAVRTLIAALSAVRVVALTPGWYDVAFKTPLMDTSKARRTLGWEPARSSAE
SARELIEGLADGAVGTSAAMGWRLRPRRDVRAAVDRAHDVTLVSWGALALLRAAGMRRARVVDAVVVASNLATGTPMALD
RILERRADPVALLAPVAVLAALGATLCGGWPAVAVTGALQVLRMSERNQRKAKLVSNAEVPAGSRG

Sequences:

>Translated_466_residues
MTAEPSRIVVTGASGNVGTGVLRALASQLPDAEVVGVCRRPPTDGEPYERVRWHTVDLSAPSAVADLEPALRGADVVIHL
ALAVHPVRDEHYLYRANVLGTQALLKAMTAAGVRQLVYASSLGIYAPGSGPPVTEDWPTTGQTTSVYSRHKVMVEQVLDE
FELDHPEVTVSRFRPTVVVQREAAWLIKSLYLGPLVPRSVLELLRRRELPVLPVPAPLALQFVHADDVGDAVVRMVTQQV
RGSFNIAADVLDTAALADLVGARPVSVPPQAVRTLIAALSAVRVVALTPGWYDVAFKTPLMDTSKARRTLGWEPARSSAE
SARELIEGLADGAVGTSAAMGWRLRPRRDVRAAVDRAHDVTLVSWGALALLRAAGMRRARVVDAVVVASNLATGTPMALD
RILERRADPVALLAPVAVLAALGATLCGGWPAVAVTGALQVLRMSERNQRKAKLVSNAEVPAGSRG
>Mature_465_residues
TAEPSRIVVTGASGNVGTGVLRALASQLPDAEVVGVCRRPPTDGEPYERVRWHTVDLSAPSAVADLEPALRGADVVIHLA
LAVHPVRDEHYLYRANVLGTQALLKAMTAAGVRQLVYASSLGIYAPGSGPPVTEDWPTTGQTTSVYSRHKVMVEQVLDEF
ELDHPEVTVSRFRPTVVVQREAAWLIKSLYLGPLVPRSVLELLRRRELPVLPVPAPLALQFVHADDVGDAVVRMVTQQVR
GSFNIAADVLDTAALADLVGARPVSVPPQAVRTLIAALSAVRVVALTPGWYDVAFKTPLMDTSKARRTLGWEPARSSAES
ARELIEGLADGAVGTSAAMGWRLRPRRDVRAAVDRAHDVTLVSWGALALLRAAGMRRARVVDAVVVASNLATGTPMALDR
ILERRADPVALLAPVAVLAALGATLCGGWPAVAVTGALQVLRMSERNQRKAKLVSNAEVPAGSRG

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 49649; Mature: 49518

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEPSRIVVTGASGNVGTGVLRALASQLPDAEVVGVCRRPPTDGEPYERVRWHTVDLSA
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHCEEEEEECCC
PSAVADLEPALRGADVVIHLALAVHPVRDEHYLYRANVLGTQALLKAMTAAGVRQLVYAS
CCHHHHHHHHHCCCHHHEEHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
SLGIYAPGSGPPVTEDWPTTGQTTSVYSRHKVMVEQVLDEFELDHPEVTVSRFRPTVVVQ
CCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCEEEEE
REAAWLIKSLYLGPLVPRSVLELLRRRELPVLPVPAPLALQFVHADDVGDAVVRMVTQQV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
RGSFNIAADVLDTAALADLVGARPVSVPPQAVRTLIAALSAVRVVALTPGWYDVAFKTPL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEECCC
MDTSKARRTLGWEPARSSAESARELIEGLADGAVGTSAAMGWRLRPRRDVRAAVDRAHDV
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHCCE
TLVSWGALALLRAAGMRRARVVDAVVVASNLATGTPMALDRILERRADPVALLAPVAVLA
EEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
ALGATLCGGWPAVAVTGALQVLRMSERNQRKAKLVSNAEVPAGSRG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAEPSRIVVTGASGNVGTGVLRALASQLPDAEVVGVCRRPPTDGEPYERVRWHTVDLSA
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHCEEEEEECCC
PSAVADLEPALRGADVVIHLALAVHPVRDEHYLYRANVLGTQALLKAMTAAGVRQLVYAS
CCHHHHHHHHHCCCHHHEEHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
SLGIYAPGSGPPVTEDWPTTGQTTSVYSRHKVMVEQVLDEFELDHPEVTVSRFRPTVVVQ
CCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCEEEEE
REAAWLIKSLYLGPLVPRSVLELLRRRELPVLPVPAPLALQFVHADDVGDAVVRMVTQQV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
RGSFNIAADVLDTAALADLVGARPVSVPPQAVRTLIAALSAVRVVALTPGWYDVAFKTPL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEECCC
MDTSKARRTLGWEPARSSAESARELIEGLADGAVGTSAAMGWRLRPRRDVRAAVDRAHDV
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHCCE
TLVSWGALALLRAAGMRRARVVDAVVVASNLATGTPMALDRILERRADPVALLAPVAVLA
EEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
ALGATLCGGWPAVAVTGALQVLRMSERNQRKAKLVSNAEVPAGSRG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA