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The map label for this gene is baiB [H]

Identifier: 108797157

GI number: 108797157

Start: 196381

End: 197901

Strand: Direct

Name: baiB [H]

Synonym: Mmcs_0177

Alternate gene names: 108797157

Gene position: 196381-197901 (Clockwise)

Preceding gene: 108797156

Following gene: 108797158

Centisome position: 3.44

GC content: 65.42

Gene sequence:

>1521_bases
ATGCAGATCCGCGACACCGCTGTTGCCACCCCCGACAAGCCCGCCATCATCATGTATCCCTCCGGCACGGTGGTGACGTT
CGGCGAACTCGAGGCGCGGGCGAACCGGCTCGCCCACCTGTTCCGCGATGCCGGCCTGGTCGAAGGCGACGCCGTCGCGA
TCCTGATGGAGAACAACGAGCACATCCACGCCGTGATGTGGGCGGCCCGCCGCAGCGGGCTGTACTACGTCCCGATCAAC
ACCCACCTCACCGCTGCCGAGGTCGCTTACATCGTCGACAACAGCGGTGCCAAGGCGATCGTCGGTTCGGGCAAGCTCGC
CGACACCCTGGCCGGGCTCGAAGCGGAACTCCCGCACGGGCTGCCGCCTGTCCGGCTGATCGCCGGTGCGCAACTCGACG
GCTGGCAGAGCTATCCCGACTGCGTGGCCGACAAACCCGACACCCCGATCGCCGACGAGATCGAGGGTGATCTGCTGCAG
TACTCGTCCGGCACGACCGTGCGTCCGAAGGGGATCAAACGCGAACTGCCGCATGTGCCGCCGTCGGAGTCGCCGGGCAT
GATGGCCGCGCTGGTGTCGTTCTGGATGCACCCCGACGCCGTGTACCTCAGTCCCGCACCGCTGTACCACACCGCACCCT
CGGTGTGGTCGATGCAGACCCAGGCCGGCGGTATCACCACCGTCGTGATGGAGAAGTTCGACGCCGAAGGCGCCCTCGAC
GCCATCGCAAAGTACAAGGTCACGCACGGCCAGTTCGTGCCGGTGATGTTCACCCGGATGCTGAAACTGCCGGAGGACGT
CCGCAAGTCGTACGACGTGTCGAGCCTCGAGCGGGTCATGCACGCGGCCGCTCCGTGCCCGGTGGAGATCAAGAAGCAGA
TGATCGACTGGTGGGGTCCGATCGTCGACGAGTACTACGCGTCCTCGGAGGCGCACGGGTCCACGCTGATCACCGCCGAG
GAGTGGCTGACGCATCCCGGTTCGGTCGGCCGACCCCTCACGGGTGTCCTCCACATCGTCGGTGAAGACGGCAACGAGTT
GCCGCCGGGGCAGGCCGGTGAGATCTACTTCGAGGGCGGCTACGACTTCGAGTACCTCAACGACCCCGAGAAGACCGCCT
CGTCACGGCATCCACTCGGTTGGAAGACGGTGGGCGACATCGGATATCTGGACGAGGAGGGTTATCTCTACCTCACCGAC
CGGCGCCACCACATGATCATCTCCGGCGGGGTCAACATCTATCCGCAGGAAGCCGAGAACATGTTGATCACCCATCCCAA
GGTGATGGATGCCGCGGTGTTCGGCGTCCCCGACGACGAGATGGGGCAGAGCGTCAGGGCCGTGGTCCAGACCGTGGACC
CGGCCGACGCGACCGACGCCTTCGCCGAGGAACTGATCTCCTGGCTGCGGGATCGGCTCGCGCACTACAAGTGTCCGCGG
TCGCTGTCGTTCGAACCTCAGCTGCCCCGCACGGACACCGGCAAGCTCTACAAGCAGGAGTTGATCAAGAAGTACTCGTG
A

Upstream 100 bases:

>100_bases
CGGTGCGCTTTTGACGCCCGCTCTCGGCCGCGCGACGGTGTGCTCCCGGCCATACTCGGGCTCTCGACACCTGTCAACTC
ACCCCTAGTACAGTGCTCAC

Downstream 100 bases:

>100_bases
ACGATCCGGTGGAACTGGCAGGCGGCATCCGCGTCGCGGTCGAAACCCCTTGCGACGACGCCTCGTTCACGCTGACCGAG
GCGCCGTCCGACGATCCGCG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MQIRDTAVATPDKPAIIMYPSGTVVTFGELEARANRLAHLFRDAGLVEGDAVAILMENNEHIHAVMWAARRSGLYYVPIN
THLTAAEVAYIVDNSGAKAIVGSGKLADTLAGLEAELPHGLPPVRLIAGAQLDGWQSYPDCVADKPDTPIADEIEGDLLQ
YSSGTTVRPKGIKRELPHVPPSESPGMMAALVSFWMHPDAVYLSPAPLYHTAPSVWSMQTQAGGITTVVMEKFDAEGALD
AIAKYKVTHGQFVPVMFTRMLKLPEDVRKSYDVSSLERVMHAAAPCPVEIKKQMIDWWGPIVDEYYASSEAHGSTLITAE
EWLTHPGSVGRPLTGVLHIVGEDGNELPPGQAGEIYFEGGYDFEYLNDPEKTASSRHPLGWKTVGDIGYLDEEGYLYLTD
RRHHMIISGGVNIYPQEAENMLITHPKVMDAAVFGVPDDEMGQSVRAVVQTVDPADATDAFAEELISWLRDRLAHYKCPR
SLSFEPQLPRTDTGKLYKQELIKKYS

Sequences:

>Translated_506_residues
MQIRDTAVATPDKPAIIMYPSGTVVTFGELEARANRLAHLFRDAGLVEGDAVAILMENNEHIHAVMWAARRSGLYYVPIN
THLTAAEVAYIVDNSGAKAIVGSGKLADTLAGLEAELPHGLPPVRLIAGAQLDGWQSYPDCVADKPDTPIADEIEGDLLQ
YSSGTTVRPKGIKRELPHVPPSESPGMMAALVSFWMHPDAVYLSPAPLYHTAPSVWSMQTQAGGITTVVMEKFDAEGALD
AIAKYKVTHGQFVPVMFTRMLKLPEDVRKSYDVSSLERVMHAAAPCPVEIKKQMIDWWGPIVDEYYASSEAHGSTLITAE
EWLTHPGSVGRPLTGVLHIVGEDGNELPPGQAGEIYFEGGYDFEYLNDPEKTASSRHPLGWKTVGDIGYLDEEGYLYLTD
RRHHMIISGGVNIYPQEAENMLITHPKVMDAAVFGVPDDEMGQSVRAVVQTVDPADATDAFAEELISWLRDRLAHYKCPR
SLSFEPQLPRTDTGKLYKQELIKKYS
>Mature_506_residues
MQIRDTAVATPDKPAIIMYPSGTVVTFGELEARANRLAHLFRDAGLVEGDAVAILMENNEHIHAVMWAARRSGLYYVPIN
THLTAAEVAYIVDNSGAKAIVGSGKLADTLAGLEAELPHGLPPVRLIAGAQLDGWQSYPDCVADKPDTPIADEIEGDLLQ
YSSGTTVRPKGIKRELPHVPPSESPGMMAALVSFWMHPDAVYLSPAPLYHTAPSVWSMQTQAGGITTVVMEKFDAEGALD
AIAKYKVTHGQFVPVMFTRMLKLPEDVRKSYDVSSLERVMHAAAPCPVEIKKQMIDWWGPIVDEYYASSEAHGSTLITAE
EWLTHPGSVGRPLTGVLHIVGEDGNELPPGQAGEIYFEGGYDFEYLNDPEKTASSRHPLGWKTVGDIGYLDEEGYLYLTD
RRHHMIISGGVNIYPQEAENMLITHPKVMDAAVFGVPDDEMGQSVRAVVQTVDPADATDAFAEELISWLRDRLAHYKCPR
SLSFEPQLPRTDTGKLYKQELIKKYS

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=548, Percent_Identity=26.2773722627737, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI42544132, Length=506, Percent_Identity=27.0750988142292, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI157311624, Length=501, Percent_Identity=26.1477045908184, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI157311622, Length=501, Percent_Identity=26.1477045908184, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI122937307, Length=502, Percent_Identity=27.8884462151394, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI187761345, Length=527, Percent_Identity=26.5654648956357, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI187761343, Length=527, Percent_Identity=26.5654648956357, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI58082049, Length=501, Percent_Identity=26.7465069860279, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=26.9005847953216, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=26.5748031496063, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI40807357, Length=546, Percent_Identity=26.5567765567766, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI38524616, Length=545, Percent_Identity=25.6880733944954, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI28416953, Length=530, Percent_Identity=22.8301886792453, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI13375727, Length=579, Percent_Identity=24.3523316062176, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI8923896, Length=553, Percent_Identity=23.869801084991, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI253970489, Length=566, Percent_Identity=23.1448763250883, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788107, Length=523, Percent_Identity=27.7246653919694, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI221142682, Length=522, Percent_Identity=28.544061302682, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI145693145, Length=520, Percent_Identity=26.3461538461538, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786810, Length=503, Percent_Identity=23.6580516898608, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790505, Length=524, Percent_Identity=23.8549618320611, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788595, Length=480, Percent_Identity=23.75, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=541, Percent_Identity=25.5083179297597, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI71994694, Length=541, Percent_Identity=25.5083179297597, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=27.7777777777778, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI71994690, Length=541, Percent_Identity=25.5083179297597, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=513, Percent_Identity=26.9005847953216, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=25.9124087591241, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=496, Percent_Identity=25, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=378, Percent_Identity=28.8359788359788, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17551278, Length=569, Percent_Identity=26.5377855887522, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=528, Percent_Identity=26.1363636363636, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=527, Percent_Identity=26.1859582542694, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI115534440, Length=547, Percent_Identity=25.5941499085923, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=399, Percent_Identity=26.5664160401003, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319699, Length=538, Percent_Identity=25.4646840148699, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323182, Length=573, Percent_Identity=23.3856893542757, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=550, Percent_Identity=23.6363636363636, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355181, Length=487, Percent_Identity=27.3100616016427, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18859661, Length=503, Percent_Identity=27.037773359841, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24581924, Length=560, Percent_Identity=27.1428571428571, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24648253, Length=476, Percent_Identity=25, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648255, Length=476, Percent_Identity=24.7899159663866, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24656500, Length=519, Percent_Identity=24.6628131021195, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI20130357, Length=488, Percent_Identity=24.7950819672131, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI21358303, Length=478, Percent_Identity=23.2217573221757, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356947, Length=509, Percent_Identity=25.147347740668, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24648260, Length=508, Percent_Identity=25, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922652, Length=516, Percent_Identity=24.4186046511628, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356441, Length=446, Percent_Identity=23.9910313901345, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281365686, Length=487, Percent_Identity=23.2032854209446, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648257, Length=516, Percent_Identity=22.8682170542636, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24653035, Length=499, Percent_Identity=22.8456913827655, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355225, Length=536, Percent_Identity=24.6268656716418, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161076582, Length=509, Percent_Identity=21.2180746561886, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62472339, Length=538, Percent_Identity=22.8624535315985, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24667955, Length=538, Percent_Identity=22.8624535315985, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667959, Length=372, Percent_Identity=25, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 55594; Mature: 55594

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRDTAVATPDKPAIIMYPSGTVVTFGELEARANRLAHLFRDAGLVEGDAVAILMENNE
CCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
HIHAVMWAARRSGLYYVPINTHLTAAEVAYIVDNSGAKAIVGSGKLADTLAGLEAELPHG
EEEEEEEHHHHCCEEEEECCCCCCHHEEEEEEECCCCEEEEECCHHHHHHHCCHHCCCCC
LPPVRLIAGAQLDGWQSYPDCVADKPDTPIADEIEGDLLQYSSGTTVRPKGIKRELPHVP
CCCEEEEECCCCCCCCCCCHHHCCCCCCCCHHHHCCCCEEECCCCEECCCCHHHHCCCCC
PSESPGMMAALVSFWMHPDAVYLSPAPLYHTAPSVWSMQTQAGGITTVVMEKFDAEGALD
CCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEECCCCCEEEEEHHHHCCCHHHH
AIAKYKVTHGQFVPVMFTRMLKLPEDVRKSYDVSSLERVMHAAAPCPVEIKKQMIDWWGP
HHHHHCCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IVDEYYASSEAHGSTLITAEEWLTHPGSVGRPLTGVLHIVGEDGNELPPGQAGEIYFEGG
HHHHHHCCCCCCCCEEEEHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECC
YDFEYLNDPEKTASSRHPLGWKTVGDIGYLDEEGYLYLTDRRHHMIISGGVNIYPQEAEN
CCCCCCCCHHHHHCCCCCCCCEEECCCCEECCCCEEEEECCCEEEEEECCCEECCCCCCC
MLITHPKVMDAAVFGVPDDEMGQSVRAVVQTVDPADATDAFAEELISWLRDRLAHYKCPR
EEEECCHHHHHEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
SLSFEPQLPRTDTGKLYKQELIKKYS
CCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQIRDTAVATPDKPAIIMYPSGTVVTFGELEARANRLAHLFRDAGLVEGDAVAILMENNE
CCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
HIHAVMWAARRSGLYYVPINTHLTAAEVAYIVDNSGAKAIVGSGKLADTLAGLEAELPHG
EEEEEEEHHHHCCEEEEECCCCCCHHEEEEEEECCCCEEEEECCHHHHHHHCCHHCCCCC
LPPVRLIAGAQLDGWQSYPDCVADKPDTPIADEIEGDLLQYSSGTTVRPKGIKRELPHVP
CCCEEEEECCCCCCCCCCCHHHCCCCCCCCHHHHCCCCEEECCCCEECCCCHHHHCCCCC
PSESPGMMAALVSFWMHPDAVYLSPAPLYHTAPSVWSMQTQAGGITTVVMEKFDAEGALD
CCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEECCCCCEEEEEHHHHCCCHHHH
AIAKYKVTHGQFVPVMFTRMLKLPEDVRKSYDVSSLERVMHAAAPCPVEIKKQMIDWWGP
HHHHHCCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IVDEYYASSEAHGSTLITAEEWLTHPGSVGRPLTGVLHIVGEDGNELPPGQAGEIYFEGG
HHHHHHCCCCCCCCEEEEHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECC
YDFEYLNDPEKTASSRHPLGWKTVGDIGYLDEEGYLYLTDRRHHMIISGGVNIYPQEAEN
CCCCCCCCHHHHHCCCCCCCCEEECCCCEECCCCEEEEECCCEEEEEECCCEECCCCCCC
MLITHPKVMDAAVFGVPDDEMGQSVRAVVQTVDPADATDAFAEELISWLRDRLAHYKCPR
EEEECCHHHHHEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
SLSFEPQLPRTDTGKLYKQELIKKYS
CCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]