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The map label for this gene is murF [H]

Identifier: 108762254

GI number: 108762254

Start: 6971358

End: 6972758

Strand: Reverse

Name: murF [H]

Synonym: MXAN_5608

Alternate gene names: 108762254

Gene position: 6972758-6971358 (Counterclockwise)

Preceding gene: 108761901

Following gene: 108762428

Centisome position: 76.29

GC content: 72.81

Gene sequence:

>1401_bases
ATGGCAGCTTCCTTCAGCGACGAAGAGGTGGTGCAGGCGACCGGGGCGACCCGGCGTGGCGGCCCGGCCCCGGCCGCCTA
CGCTACCGTCTGTACCGACACGCGGGCACTCACCCCCGGGTGCCTCTTCGTGGCCCTGGTGGGTGAGCGCTTCGACGCCC
ACGCTTTCGTGGACGCCGCGGCGAAGGGCGGGGCGGCTGGCGCGGTGGTGGCACGCGGGCGCCCGTTGCCCGCGCTGCCC
GACGGGCTGACGCTCTATGAAGTGGATGACACCCTGCGGGCCCTGGGCGCGCTGGGACGGCACCACCGTCAGCGCTTCCG
GATTCCGGTGTGCGCGGTGGGTGGCTCCAACGGGAAGACGACCACCAAGGAGATGGTGGGCGCCATCCTGGCCACGCGCG
GGCCGGCGCTGAAGACCGAGGGCAACCTCAATAACGAGATTGGCGTCCCGCTGACGCTCTTCCGGCTGGAGCCGCGCCAC
GTCGCGGCGGTCATCGAAGTGGGGATGAACCAGCCGGGGGAAATCGAGCGGCTGGCGCGCGTCGTCCAGCCGGACGCGGG
CGTCATCACGGTGGTCCAGCCCGAGCACCTGGAAGGGCTGGGCAGCATCGAGGGCGTGGCGGCGGCGGAGGGCGAGCTGT
TCCAGGAGATGGGGCACGGCACCACCATCGTGGTGAACGTGGACGACGCGCTCATCCCCGCGCAGGCGGAGCGCAGCGGA
GCGCAGCGGCTGACGTTCGGCCGGGCGGAGCACGCGGACGTGCGGCTCGCGGACGTGAAGACGCTGGGCCGCGAGGGCAT
GGTGGCCACGGTGCGGCACCTGGGCCGCGAGTGGCCGGTGCGGCTGCACTTCGTGGGGCCACACAACGCGCAGAACGCGA
CAGCGGCCTTCGCGGTGGCGCTGGCGCTGGGCTACACGCCGGAGGAGTGCGTGCGCGGCCTGGAGTCGGCGCGGCCGTAC
TCGCGGCGCCTCAACGTGGTGGACGGGCAGCACGGCGTGACGGTCATCGACGACTGCTACAACGCCAACCCGGCCTCCAT
GGACGCCGCGCTGGAGACGCTGGGCACGCTGGTGCCCGCGAGCGGCCGGGCCGTGGTGGTGCTGGGGGACATGCTGGAGC
TGGGGCCGGGCGAGCTCGAGGAGCACGCGCGCCTGGGCGGCCAGATTCCCTCGCACGCGGCGCTGGCGGCATTTTTCGGT
CCCCGCTCCTTGAAGGGGTGGGAGGCCGCTTCCATGGGTGAATCCGCCGCCCACTTCACCGACGTGGAGCCGCTGATGGC
GTGGTTGGCGCCGCGGCTTCGCGAAGGTGACGTGGTGCTGGTGAAGGGCAGCCGCGGCATGCGGCTGGAGCGGGTGGTGG
CCGCCCTGACGGGCACGGCCGCCCCCGGAGGAAACCACTAG

Upstream 100 bases:

>100_bases
CGACGACCGGCAGGTGGCCGCGAAGGCGCTGGCCAACCGCATCGCTGGCTGAGCCCGAGCCTCCCAAGCCCCGTCGACCC
GCCCGCCCATCTCTCACACC

Downstream 100 bases:

>100_bases
TGCTGTACCTCCTCTACGAGGTCATCCAGAACTCCGAGGCGGGGCGCGTTCTCAACTTCCTGCGCTACCCCACCTTCCGC
ATCATCGCCGCGGGCGTCTT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAASFSDEEVVQATGATRRGGPAPAAYATVCTDTRALTPGCLFVALVGERFDAHAFVDAAAKGGAAGAVVARGRPLPALP
DGLTLYEVDDTLRALGALGRHHRQRFRIPVCAVGGSNGKTTTKEMVGAILATRGPALKTEGNLNNEIGVPLTLFRLEPRH
VAAVIEVGMNQPGEIERLARVVQPDAGVITVVQPEHLEGLGSIEGVAAAEGELFQEMGHGTTIVVNVDDALIPAQAERSG
AQRLTFGRAEHADVRLADVKTLGREGMVATVRHLGREWPVRLHFVGPHNAQNATAAFAVALALGYTPEECVRGLESARPY
SRRLNVVDGQHGVTVIDDCYNANPASMDAALETLGTLVPASGRAVVVLGDMLELGPGELEEHARLGGQIPSHAALAAFFG
PRSLKGWEAASMGESAAHFTDVEPLMAWLAPRLREGDVVLVKGSRGMRLERVVAALTGTAAPGGNH

Sequences:

>Translated_466_residues
MAASFSDEEVVQATGATRRGGPAPAAYATVCTDTRALTPGCLFVALVGERFDAHAFVDAAAKGGAAGAVVARGRPLPALP
DGLTLYEVDDTLRALGALGRHHRQRFRIPVCAVGGSNGKTTTKEMVGAILATRGPALKTEGNLNNEIGVPLTLFRLEPRH
VAAVIEVGMNQPGEIERLARVVQPDAGVITVVQPEHLEGLGSIEGVAAAEGELFQEMGHGTTIVVNVDDALIPAQAERSG
AQRLTFGRAEHADVRLADVKTLGREGMVATVRHLGREWPVRLHFVGPHNAQNATAAFAVALALGYTPEECVRGLESARPY
SRRLNVVDGQHGVTVIDDCYNANPASMDAALETLGTLVPASGRAVVVLGDMLELGPGELEEHARLGGQIPSHAALAAFFG
PRSLKGWEAASMGESAAHFTDVEPLMAWLAPRLREGDVVLVKGSRGMRLERVVAALTGTAAPGGNH
>Mature_465_residues
AASFSDEEVVQATGATRRGGPAPAAYATVCTDTRALTPGCLFVALVGERFDAHAFVDAAAKGGAAGAVVARGRPLPALPD
GLTLYEVDDTLRALGALGRHHRQRFRIPVCAVGGSNGKTTTKEMVGAILATRGPALKTEGNLNNEIGVPLTLFRLEPRHV
AAVIEVGMNQPGEIERLARVVQPDAGVITVVQPEHLEGLGSIEGVAAAEGELFQEMGHGTTIVVNVDDALIPAQAERSGA
QRLTFGRAEHADVRLADVKTLGREGMVATVRHLGREWPVRLHFVGPHNAQNATAAFAVALALGYTPEECVRGLESARPYS
RRLNVVDGQHGVTVIDDCYNANPASMDAALETLGTLVPASGRAVVVLGDMLELGPGELEEHARLGGQIPSHAALAAFFGP
RSLKGWEAASMGESAAHFTDVEPLMAWLAPRLREGDVVLVKGSRGMRLERVVAALTGTAAPGGNH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=438, Percent_Identity=38.5844748858447, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1786279, Length=360, Percent_Identity=26.1111111111111, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48807; Mature: 48676

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASFSDEEVVQATGATRRGGPAPAAYATVCTDTRALTPGCLFVALVGERFDAHAFVDAA
CCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEEHHHCCCCCHHHHHHH
AKGGAAGAVVARGRPLPALPDGLTLYEVDDTLRALGALGRHHRQRFRIPVCAVGGSNGKT
CCCCCCCEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
TTKEMVGAILATRGPALKTEGNLNNEIGVPLTLFRLEPRHVAAVIEVGMNQPGEIERLAR
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHH
VVQPDAGVITVVQPEHLEGLGSIEGVAAAEGELFQEMGHGTTIVVNVDDALIPAQAERSG
HHCCCCCEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHCC
AQRLTFGRAEHADVRLADVKTLGREGMVATVRHLGREWPVRLHFVGPHNAQNATAAFAVA
CHHEECCCCCCCCEEEHHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHH
LALGYTPEECVRGLESARPYSRRLNVVDGQHGVTVIDDCYNANPASMDAALETLGTLVPA
HHHCCCHHHHHHHHHHCCCHHHCCCEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
SGRAVVVLGDMLELGPGELEEHARLGGQIPSHAALAAFFGPRSLKGWEAASMGESAAHFT
CCCEEEEECCHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHCCCCCHHHHC
DVEPLMAWLAPRLREGDVVLVKGSRGMRLERVVAALTGTAAPGGNH
CHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AASFSDEEVVQATGATRRGGPAPAAYATVCTDTRALTPGCLFVALVGERFDAHAFVDAA
CCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEEHHHCCCCCHHHHHHH
AKGGAAGAVVARGRPLPALPDGLTLYEVDDTLRALGALGRHHRQRFRIPVCAVGGSNGKT
CCCCCCCEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
TTKEMVGAILATRGPALKTEGNLNNEIGVPLTLFRLEPRHVAAVIEVGMNQPGEIERLAR
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHH
VVQPDAGVITVVQPEHLEGLGSIEGVAAAEGELFQEMGHGTTIVVNVDDALIPAQAERSG
HHCCCCCEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHCC
AQRLTFGRAEHADVRLADVKTLGREGMVATVRHLGREWPVRLHFVGPHNAQNATAAFAVA
CHHEECCCCCCCCEEEHHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHH
LALGYTPEECVRGLESARPYSRRLNVVDGQHGVTVIDDCYNANPASMDAALETLGTLVPA
HHHCCCHHHHHHHHHHCCCHHHCCCEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
SGRAVVVLGDMLELGPGELEEHARLGGQIPSHAALAAFFGPRSLKGWEAASMGESAAHFT
CCCEEEEECCHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHCCCCCHHHHC
DVEPLMAWLAPRLREGDVVLVKGSRGMRLERVVAALTGTAAPGGNH
CHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]