Click here to switch to the map view.

The map label for this gene is nlaIIIR [H]

Identifier: 108563575

GI number: 108563575

Start: 1217028

End: 1217276

Strand: Reverse

Name: nlaIIIR [H]

Synonym: HPAG1_1150

Alternate gene names: 108563575

Gene position: 1217276-1217028 (Counterclockwise)

Preceding gene: 108563576

Following gene: 108563574

Centisome position: 76.25

GC content: 34.14

Gene sequence:

>249_bases
ATGATTAAGAAACGCCAAATTTGTAAAGAATGCAAAGAGACTGGCTATAGATTTGATGCAACAAAAATTCCTGGCAATTA
TTATTCTTTCTATGAAGGGGAGGCTGAATATGATGGTTGTGTGGGCTGTTATCAATATGACCCCATACAATACAGGAAAA
CTTGTAATGATAGGATATTTAATGAAGGGTATCAAAAAGGTTATGGTGAGGGGTATCAAATCGGATACCATCAAAAAACT
ACTTTATAA

Upstream 100 bases:

>100_bases
ATAGAAGTGGATCATAAAGACGACCGCAAGAATGATTTAAGAGTTTCTGATTCAAACACACAGACTTTTGATGATTTTCA
GGCTTTATACAAAGCTTGTA

Downstream 100 bases:

>100_bases
CGGTTGCAATGAACTACATCGGCTCTAAATACAAGCTCATTCCTTTTATTAAAGAAAATATCCATGCGGTTGTAGGGGAC
AACCTTTCTGGTGCGATTTT

Product: iceA1

Products: NA

Alternate protein names: R.NlaIII; Endonuclease NlaIII; Type II restriction enzyme NlaIII [H]

Number of amino acids: Translated: 82; Mature: 82

Protein sequence:

>82_residues
MIKKRQICKECKETGYRFDATKIPGNYYSFYEGEAEYDGCVGCYQYDPIQYRKTCNDRIFNEGYQKGYGEGYQIGYHQKT
TL

Sequences:

>Translated_82_residues
MIKKRQICKECKETGYRFDATKIPGNYYSFYEGEAEYDGCVGCYQYDPIQYRKTCNDRIFNEGYQKGYGEGYQIGYHQKT
TL
>Mature_82_residues
MIKKRQICKECKETGYRFDATKIPGNYYSFYEGEAEYDGCVGCYQYDPIQYRKTCNDRIFNEGYQKGYGEGYQIGYHQKT
TL

Specific function: Recognizes the double-stranded sequence CATG and cleaves after G-4 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR007979 [H]

Pfam domain/function: PF05315 ICEA [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 9718; Mature: 9718

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
6.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKRQICKECKETGYRFDATKIPGNYYSFYEGEAEYDGCVGCYQYDPIQYRKTCNDRIF
CCCHHHHHHHHHHCCCCCCCEECCCCCCEEECCCCCCCCEEECEECCCHHHHHHHHHHHH
NEGYQKGYGEGYQIGYHQKTTL
HHHHHCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MIKKRQICKECKETGYRFDATKIPGNYYSFYEGEAEYDGCVGCYQYDPIQYRKTCNDRIF
CCCHHHHHHHHHHCCCCCCCEECCCCCCEEECCCCCCCCEEECEECCCHHHHHHHHHHHH
NEGYQKGYGEGYQIGYHQKTTL
HHHHHCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8996109 [H]