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The map label for this gene is nlaIIIM [H]

Identifier: 108563574

GI number: 108563574

Start: 1216030

End: 1217019

Strand: Reverse

Name: nlaIIIM [H]

Synonym: HPAG1_1149

Alternate gene names: 108563574

Gene position: 1217019-1216030 (Counterclockwise)

Preceding gene: 108563575

Following gene: 108563573

Centisome position: 76.24

GC content: 36.87

Gene sequence:

>990_bases
ATGAACTACATCGGCTCTAAATACAAGCTCATTCCTTTTATTAAAGAAAATATCCATGCGGTTGTAGGGGACAACCTTTC
TGGTGCGATTTTTTGTGATCTGTTCGCTGGGACGGGCATTGTGGGGCGTGCATTTAAAAAAGCTGTTAATAAGGTTATTT
CTAATGATTTGGAATATTATAGCTTTGTTTTGAATCAAAATTATATCGGCAACATTCAAGAAATCCCTAATCAAGAAGAG
CTTATTGATAGGCTCAATAGCGTTGCTTTAAAAAAGGGTTTTATTCATTCGCATTATTCTTTAGGGGGGAGTTCAAGGCA
GTATTTTAGCGAAACAAACGCTCAAAAAATTGATGCGATGCGTTTAAAAATTGAAGAGCTTAAACTTTCTCAAAACATTG
ATAATTGTGCGTATTATTTTTTGCTCGCATCACTATTAGAGAGCGCGGACAAGGTGGCTAACACCGCTTCAGTTTATGGG
GCTTTTTTAAAACGCCTTAAAAAAAGCGCTCAAAAAGAACTCATCTTAAAAGGCGCTCATTTTGATGTGAGTTTAAACGC
TAATGAAGTGTATCAGCAAGATTCTAGCGAGTTGATCGGAAAGATTTCAGGGGATATTTTGTATTTAGACCCTCCTTATA
ATGCGAGGCAATACGGGGCGAATTACCATTTATTAAACACGATTGCTATTTATACGCCCTTTGCTCCAAAAGGCAAAACC
GGCTTGCCCAGTTACCAGAAATCATCGTTTTGCTCTCGCGCTAAAATCTTAAACGCTTTTGAAAATTTAATCAAAAAATC
GCGATTCAAATACATCTTTTTAAGCTATAACAATGAAGGGCTTATGAGTGAAACAGAGATTGGAAATATTTTAAAAAAAT
ACGGCGCTTACTCCTTAATGACCAAAACTTACATGCGTTTTAAAGCGGATAACAAACGTGCCCATAAAGCCGCGCACACC
AAAGAGTGTTTGCATACTCTTATCAAATAA

Upstream 100 bases:

>100_bases
AAACTTGTAATGATAGGATATTTAATGAAGGGTATCAAAAAGGTTATGGTGAGGGGTATCAAATCGGATACCATCAAAAA
ACTACTTTATAACGGTTGCA

Downstream 100 bases:

>100_bases
GAGTCAAAATAGCCCAACCTTTAAATGGCAAAAATAATCGCTTAAATACCGCTTGAATAAGGTAAAATGAATCCAAAATA
AGCCTATAAAAAAGGGGAAA

Product: type II adenine specific DNA methyltransferase

Products: NA

Alternate protein names: M.NlaIII; Adenine-specific methyltransferase NlaIII [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNYIGSKYKLIPFIKENIHAVVGDNLSGAIFCDLFAGTGIVGRAFKKAVNKVISNDLEYYSFVLNQNYIGNIQEIPNQEE
LIDRLNSVALKKGFIHSHYSLGGSSRQYFSETNAQKIDAMRLKIEELKLSQNIDNCAYYFLLASLLESADKVANTASVYG
AFLKRLKKSAQKELILKGAHFDVSLNANEVYQQDSSELIGKISGDILYLDPPYNARQYGANYHLLNTIAIYTPFAPKGKT
GLPSYQKSSFCSRAKILNAFENLIKKSRFKYIFLSYNNEGLMSETEIGNILKKYGAYSLMTKTYMRFKADNKRAHKAAHT
KECLHTLIK

Sequences:

>Translated_329_residues
MNYIGSKYKLIPFIKENIHAVVGDNLSGAIFCDLFAGTGIVGRAFKKAVNKVISNDLEYYSFVLNQNYIGNIQEIPNQEE
LIDRLNSVALKKGFIHSHYSLGGSSRQYFSETNAQKIDAMRLKIEELKLSQNIDNCAYYFLLASLLESADKVANTASVYG
AFLKRLKKSAQKELILKGAHFDVSLNANEVYQQDSSELIGKISGDILYLDPPYNARQYGANYHLLNTIAIYTPFAPKGKT
GLPSYQKSSFCSRAKILNAFENLIKKSRFKYIFLSYNNEGLMSETEIGNILKKYGAYSLMTKTYMRFKADNKRAHKAAHT
KECLHTLIK
>Mature_329_residues
MNYIGSKYKLIPFIKENIHAVVGDNLSGAIFCDLFAGTGIVGRAFKKAVNKVISNDLEYYSFVLNQNYIGNIQEIPNQEE
LIDRLNSVALKKGFIHSHYSLGGSSRQYFSETNAQKIDAMRLKIEELKLSQNIDNCAYYFLLASLLESADKVANTASVYG
AFLKRLKKSAQKELILKGAHFDVSLNANEVYQQDSSELIGKISGDILYLDPPYNARQYGANYHLLNTIAIYTPFAPKGKT
GLPSYQKSSFCSRAKILNAFENLIKKSRFKYIFLSYNNEGLMSETEIGNILKKYGAYSLMTKTYMRFKADNKRAHKAAHT
KECLHTLIK

Specific function: This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease [H]

COG id: COG3392

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 37139; Mature: 37139

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIGSKYKLIPFIKENIHAVVGDNLSGAIFCDLFAGTGIVGRAFKKAVNKVISNDLEYY
CCCCCCCEEEHHHHHHCHHEEECCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHCCHHHH
SFVLNQNYIGNIQEIPNQEELIDRLNSVALKKGFIHSHYSLGGSSRQYFSETNAQKIDAM
HHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHH
RLKIEELKLSQNIDNCAYYFLLASLLESADKVANTASVYGAFLKRLKKSAQKELILKGAH
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
FDVSLNANEVYQQDSSELIGKISGDILYLDPPYNARQYGANYHLLNTIAIYTPFAPKGKT
EEEECCHHHHHHCCHHHHHHHCCCCEEEECCCCCHHHHCCCEEEEEEEEEECCCCCCCCC
GLPSYQKSSFCSRAKILNAFENLIKKSRFKYIFLSYNNEGLMSETEIGNILKKYGAYSLM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHH
TKTYMRFKADNKRAHKAAHTKECLHTLIK
HHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYIGSKYKLIPFIKENIHAVVGDNLSGAIFCDLFAGTGIVGRAFKKAVNKVISNDLEYY
CCCCCCCEEEHHHHHHCHHEEECCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHCCHHHH
SFVLNQNYIGNIQEIPNQEELIDRLNSVALKKGFIHSHYSLGGSSRQYFSETNAQKIDAM
HHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHH
RLKIEELKLSQNIDNCAYYFLLASLLESADKVANTASVYGAFLKRLKKSAQKELILKGAH
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
FDVSLNANEVYQQDSSELIGKISGDILYLDPPYNARQYGANYHLLNTIAIYTPFAPKGKT
EEEECCHHHHHHCCHHHHHHHCCCCEEEECCCCCHHHHCCCEEEEEEEEEECCCCCCCCC
GLPSYQKSSFCSRAKILNAFENLIKKSRFKYIFLSYNNEGLMSETEIGNILKKYGAYSLM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHH
TKTYMRFKADNKRAHKAAHTKECLHTLIK
HHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2277628 [H]