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The map label for this gene is nlaIIIR [H]

Identifier: 108563576

GI number: 108563576

Start: 1217269

End: 1217715

Strand: Reverse

Name: nlaIIIR [H]

Synonym: HPAG1_1151

Alternate gene names: 108563576

Gene position: 1217715-1217269 (Counterclockwise)

Preceding gene: 108563577

Following gene: 108563575

Centisome position: 76.28

GC content: 36.47

Gene sequence:

>447_bases
ATGAGTAAAAGTAAGAAAGAGTTATTTTTGGAACTTGCACAACCTGATAAAAATGGGGTGAGTCGTTGGGCAAGCGTTAC
AGAATTTGTAGGAAAATACCAAGGGTTACAGCTAGGTAATGGGGGGAGTTGGTTCAGGAATAACTCATCTTTGGCTAAAG
AATTTAAATTAGAATTTGATAAAGGGCAAACTCTAGGGAATTCTATTGATAGAATACGCTTGAATGGCTATAATACCGAA
TGCGTTTTTAATCAAAGTATCCGTCAAGACATTAAAAACTACTACAAGCAACAATGTTGCACGATGTGCGGTGCACGCGG
CAATTCTGAAAACACTCAAATAGAAGTGGATCATAAAGACGACCGCAAGAATGATTTAAGAGTTTCTGATTCAAACACAC
AGACTTTTGATGATTTTCAGGCTTTATACAAAGCTTGTAATGATTAA

Upstream 100 bases:

>100_bases
TACAAAGGATCGTTAATTTTAAATAAGCGGTTGGAGTTTGCGCCATGCGGGTTATTTTGAGGTAAATTGATGAGAATTTT
TTATAGAGAGGATTTTTAAA

Downstream 100 bases:

>100_bases
GAAACGCCAAATTTGTAAAGAATGCAAAGAGACTGGCTATAGATTTGATGCAACAAAAATTCCTGGCAATTATTATTCTT
TCTATGAAGGGGAGGCTGAA

Product: iceA1

Products: NA

Alternate protein names: R.NlaIII; Endonuclease NlaIII; Type II restriction enzyme NlaIII [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSKSKKELFLELAQPDKNGVSRWASVTEFVGKYQGLQLGNGGSWFRNNSSLAKEFKLEFDKGQTLGNSIDRIRLNGYNTE
CVFNQSIRQDIKNYYKQQCCTMCGARGNSENTQIEVDHKDDRKNDLRVSDSNTQTFDDFQALYKACND

Sequences:

>Translated_148_residues
MSKSKKELFLELAQPDKNGVSRWASVTEFVGKYQGLQLGNGGSWFRNNSSLAKEFKLEFDKGQTLGNSIDRIRLNGYNTE
CVFNQSIRQDIKNYYKQQCCTMCGARGNSENTQIEVDHKDDRKNDLRVSDSNTQTFDDFQALYKACND
>Mature_147_residues
SKSKKELFLELAQPDKNGVSRWASVTEFVGKYQGLQLGNGGSWFRNNSSLAKEFKLEFDKGQTLGNSIDRIRLNGYNTEC
VFNQSIRQDIKNYYKQQCCTMCGARGNSENTQIEVDHKDDRKNDLRVSDSNTQTFDDFQALYKACND

Specific function: Recognizes the double-stranded sequence CATG and cleaves after G-4 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR007979 [H]

Pfam domain/function: PF05315 ICEA [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 16945; Mature: 16814

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSKKELFLELAQPDKNGVSRWASVTEFVGKYQGLQLGNGGSWFRNNSSLAKEFKLEFD
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHCCC
KGQTLGNSIDRIRLNGYNTECVFNQSIRQDIKNYYKQQCCTMCGARGNSENTQIEVDHKD
CCCCCCCCCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
DRKNDLRVSDSNTQTFDDFQALYKACND
CCCCCCEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSKKELFLELAQPDKNGVSRWASVTEFVGKYQGLQLGNGGSWFRNNSSLAKEFKLEFD
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHCCC
KGQTLGNSIDRIRLNGYNTECVFNQSIRQDIKNYYKQQCCTMCGARGNSENTQIEVDHKD
CCCCCCCCCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
DRKNDLRVSDSNTQTFDDFQALYKACND
CCCCCCEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8996109 [H]