Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 108563436

GI number: 108563436

Start: 1065167

End: 1065991

Strand: Direct

Name: hemK [H]

Synonym: HPAG1_1011

Alternate gene names: 108563436

Gene position: 1065167-1065991 (Clockwise)

Preceding gene: 108563435

Following gene: 108563437

Centisome position: 66.72

GC content: 39.03

Gene sequence:

>825_bases
ATGACCCTTTCACAAGCCCTAAACAAAGCCAAAAAAGGATTATCGCAAAAAGGCTTTAGGGGGGGGTTAGAGTCTGAAAT
TTTATTAGGCTTTGTCTTGCAAAAAGAGAGGGTTTTTTTGCACACGCATGAGCATTTGGAATTAACCCACGAAGAAGAAA
CGCACTTTTTTGAATTGGTAGAAAAGCGTTTGAATGACTGCCCCATAGAGTATTTATTAGGAAGCTGTGATTTTTATGGG
CGTTCTTTTTTCGTGAATGAGCATGTTTTAATCCCACGGCCTGAAACCGAGATTTTGGTCCAAAAAGCCCTTGATATTAT
TTCTCAATACCATTTAAAAGAAATAGGCGAAATCGGTGTAGGGAGCGGATGCGTGTCCATTAGTTTGGCTTTAGAAAACC
CTAAACTTTCCATTCATGCGAGCGATATTTCATTAAAAGCTTTAGAAGTGGCGTCCAAAAATATTGAACGCTTTTGTTTA
AAAGAGCGTGTTTTTTTAAAACAAACGCGCCTTTGGGATCGCATGCCAACGATAGAAATGCTTGTCTCTAACCCGCCCTA
TATCGCTAGGGATTATCCTTTGGAAAAATCCGTTCTCAAAGAACCGCACGAAGCCCTTTTTGGGGGGGTTAAAGGCGATG
AAATCTTAAAAGAAATCATCTTTTTAGCCGCTGAGTTAAAAATCCCTTTTTTGGTTTGTGAAATGGGGTATGACCAGTTA
AAGAGCTTGAAAGAATGCTTGGAATTTTGCGGTTATGATGCAGAGTTTTACAAGGATTTGAGCGGCTTTGATAGAGGGTT
TATAGGCGTTTTAAAAAATTTTTAA

Upstream 100 bases:

>100_bases
AGAATAAAGCGTTCCCCTATTCGCACCCTTTTTATGTTTTCTTGCATTTCACCCACCCGCCCCTATTAGAGCGCTTGAAA
GCTTTGGATTATGAAATTGA

Downstream 100 bases:

>100_bases
GATAAAGTTAAAACTTAATTACCCTTTTAGTGTTACAATAAAAACACTTAAAATAATAAAGGATACCGCTCATGTATATT
GAAAAAATTCTCCAATCTTT

Product: S-adenosylmethionine-dependent methyltransferase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTLSQALNKAKKGLSQKGFRGGLESEILLGFVLQKERVFLHTHEHLELTHEEETHFFELVEKRLNDCPIEYLLGSCDFYG
RSFFVNEHVLIPRPETEILVQKALDIISQYHLKEIGEIGVGSGCVSISLALENPKLSIHASDISLKALEVASKNIERFCL
KERVFLKQTRLWDRMPTIEMLVSNPPYIARDYPLEKSVLKEPHEALFGGVKGDEILKEIIFLAAELKIPFLVCEMGYDQL
KSLKECLEFCGYDAEFYKDLSGFDRGFIGVLKNF

Sequences:

>Translated_274_residues
MTLSQALNKAKKGLSQKGFRGGLESEILLGFVLQKERVFLHTHEHLELTHEEETHFFELVEKRLNDCPIEYLLGSCDFYG
RSFFVNEHVLIPRPETEILVQKALDIISQYHLKEIGEIGVGSGCVSISLALENPKLSIHASDISLKALEVASKNIERFCL
KERVFLKQTRLWDRMPTIEMLVSNPPYIARDYPLEKSVLKEPHEALFGGVKGDEILKEIIFLAAELKIPFLVCEMGYDQL
KSLKECLEFCGYDAEFYKDLSGFDRGFIGVLKNF
>Mature_273_residues
TLSQALNKAKKGLSQKGFRGGLESEILLGFVLQKERVFLHTHEHLELTHEEETHFFELVEKRLNDCPIEYLLGSCDFYGR
SFFVNEHVLIPRPETEILVQKALDIISQYHLKEIGEIGVGSGCVSISLALENPKLSIHASDISLKALEVASKNIERFCLK
ERVFLKQTRLWDRMPTIEMLVSNPPYIARDYPLEKSVLKEPHEALFGGVKGDEILKEIIFLAAELKIPFLVCEMGYDQLK
SLKECLEFCGYDAEFYKDLSGFDRGFIGVLKNF

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=220, Percent_Identity=31.3636363636364, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787463, Length=253, Percent_Identity=29.6442687747036, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082085, Length=197, Percent_Identity=34.010152284264, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 31289; Mature: 31158

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSQALNKAKKGLSQKGFRGGLESEILLGFVLQKERVFLHTHEHLELTHEEETHFFELV
CCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHEECCCHHHHHHHHH
EKRLNDCPIEYLLGSCDFYGRSFFVNEHVLIPRPETEILVQKALDIISQYHLKEIGEIGV
HHHHCCCCHHHHHHCCCCCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GSGCVSISLALENPKLSIHASDISLKALEVASKNIERFCLKERVFLKQTRLWDRMPTIEM
CCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LVSNPPYIARDYPLEKSVLKEPHEALFGGVKGDEILKEIIFLAAELKIPFLVCEMGYDQL
HCCCCCCEECCCCCCHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEECCCHHHH
KSLKECLEFCGYDAEFYKDLSGFDRGFIGVLKNF
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
TLSQALNKAKKGLSQKGFRGGLESEILLGFVLQKERVFLHTHEHLELTHEEETHFFELV
CHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHEECCCHHHHHHHHH
EKRLNDCPIEYLLGSCDFYGRSFFVNEHVLIPRPETEILVQKALDIISQYHLKEIGEIGV
HHHHCCCCHHHHHHCCCCCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GSGCVSISLALENPKLSIHASDISLKALEVASKNIERFCLKERVFLKQTRLWDRMPTIEM
CCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LVSNPPYIARDYPLEKSVLKEPHEALFGGVKGDEILKEIIFLAAELKIPFLVCEMGYDQL
HCCCCCCEECCCCCCHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEECCCHHHH
KSLKECLEFCGYDAEFYKDLSGFDRGFIGVLKNF
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA