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The map label for this gene is gdhA [H]

Identifier: 108563437

GI number: 108563437

Start: 1066063

End: 1067409

Strand: Direct

Name: gdhA [H]

Synonym: HPAG1_1012

Alternate gene names: 108563437

Gene position: 1066063-1067409 (Clockwise)

Preceding gene: 108563436

Following gene: 108563442

Centisome position: 66.78

GC content: 44.25

Gene sequence:

>1347_bases
ATGTATATTGAAAAAATTCTCCAATCTTTACAGAAAAAATACCCCTATCAAAAAGAGTTCCATCAAGCCGTTTATGAAGC
TATCACTTCTTTAAAACCCCTTTTAGACAGCGATAAAAGCTATGAAAAGCATGCGATTTTAGAGCGTTTGATTGAGCCTG
AAAGGGAGATTTTTTTTAGGGTGTGTTGGCTAGATGATAACCATCAGATTCAAGTCAATCGGGGGTGTAGGGTTGAATTC
AATTCAGCGATTGGCCCTTATAAGGGGGGTTTGAGATTCCACCCTAGCGTGAATGAAAGCGTGATCAAATTTTTAGGCTT
TGAGCAAGTGTTGAAAAATTCGCTCACCACTTTGGCTATGGGGGGCGCTAAGGGGGGGAGCGATTTTGACCCTAAAGGGA
AGAGCGAGCATGAGATCATGCGTTTTTGCCAGGCGTTCATGAATGAATTATACCGCCACATTGGAGCCACGACTGATGTG
CCAGCTGGGGATATTGGAGTGGGCGAAAGAGAGATTGGCTATTTGTTTGGGCAATACAAAAAATTAGTCAATCGTTTTGA
GGGCGTATTGACCGGTAAAGGACTCACTTATGGGGGGAGTTTGTGCAGAAAAGAAGCTACCGGCTATGGGTGCGTGTATT
TTGCTGAAGAAATGCTGCAAGAAAGGAACAGCTCTTTAGAGGGTAAGGTTTGCAGCGTTTCTGGGAGCGGTAATGTCGCT
ATTTATACCATTGAAAAACTGCTTCAAATAGGAGCCAAACCGGTAACGGCGAGCGATTCTAATGGCATGATTTATGATAA
AGACGGCATTGATTTAGAGCTTTTGAAAGAAATTAAAGAAATCCGTCGTGGGAGGATCAAAGAATACGCTTTAGAAAAAA
AGAGCGCGAAATACACCCCCACAGAAAATTACCCCAAAGGGGGGAATGCAGTGTGGCATGTGCCTTGTTTTGCGGCTTTT
CCTAGCGCGACTGAAAATGAATTGAGCGTTTTAGACGCTAAAACCCTCCTTTCTAACGGGTGCAAATGCGTGGCTGAAGG
GGCGAACATGCCCTCAAGCAATGAAGCGATTGGATTGTTTTTGCAGGCTAAGATTTCTTATGGCATAGGCAAGGCGGCTA
ATGCTGGGGGGGTGAGCGTGAGCGGCTTGGAAATGGCGCAAAACGCGAGCATGCACCCTTGGAGTTTTGAAGTGGTGGAT
GCGAAATTACACCACATCATGAAAGAGATTTATAAGAATGTCTCTCAAACCGCTAAAGAGTTTAAAGACCCTACTAATTT
TGTTTTAGGGGCTAATATCGCTGGTTTTAGAAAAGTGGCGTCTGCGATGATAGCCCAAGGGGTTTGA

Upstream 100 bases:

>100_bases
GGTTTATAGGCGTTTTAAAAAATTTTTAAGATAAAGTTAAAACTTAATTACCCTTTTAGTGTTACAATAAAAACACTTAA
AATAATAAAGGATACCGCTC

Downstream 100 bases:

>100_bases
TTACCCCCCAATTTTACAAACCCAATTTTTTAACCCACTTTCTCACCGCGCACAACAAGGGTAAGGATTTTTGATAGGCT
TTGCGATAGATTTTAAAAGA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYEKHAILERLIEPEREIFFRVCWLDDNHQIQVNRGCRVEF
NSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAMGGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDV
PAGDIGVGEREIGYLFGQYKKLVNRFEGVLTGKGLTYGGSLCRKEATGYGCVYFAEEMLQERNSSLEGKVCSVSGSGNVA
IYTIEKLLQIGAKPVTASDSNGMIYDKDGIDLELLKEIKEIRRGRIKEYALEKKSAKYTPTENYPKGGNAVWHVPCFAAF
PSATENELSVLDAKTLLSNGCKCVAEGANMPSSNEAIGLFLQAKISYGIGKAANAGGVSVSGLEMAQNASMHPWSFEVVD
AKLHHIMKEIYKNVSQTAKEFKDPTNFVLGANIAGFRKVASAMIAQGV

Sequences:

>Translated_448_residues
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYEKHAILERLIEPEREIFFRVCWLDDNHQIQVNRGCRVEF
NSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAMGGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDV
PAGDIGVGEREIGYLFGQYKKLVNRFEGVLTGKGLTYGGSLCRKEATGYGCVYFAEEMLQERNSSLEGKVCSVSGSGNVA
IYTIEKLLQIGAKPVTASDSNGMIYDKDGIDLELLKEIKEIRRGRIKEYALEKKSAKYTPTENYPKGGNAVWHVPCFAAF
PSATENELSVLDAKTLLSNGCKCVAEGANMPSSNEAIGLFLQAKISYGIGKAANAGGVSVSGLEMAQNASMHPWSFEVVD
AKLHHIMKEIYKNVSQTAKEFKDPTNFVLGANIAGFRKVASAMIAQGV
>Mature_448_residues
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYEKHAILERLIEPEREIFFRVCWLDDNHQIQVNRGCRVEF
NSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAMGGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDV
PAGDIGVGEREIGYLFGQYKKLVNRFEGVLTGKGLTYGGSLCRKEATGYGCVYFAEEMLQERNSSLEGKVCSVSGSGNVA
IYTIEKLLQIGAKPVTASDSNGMIYDKDGIDLELLKEIKEIRRGRIKEYALEKKSAKYTPTENYPKGGNAVWHVPCFAAF
PSATENELSVLDAKTLLSNGCKCVAEGANMPSSNEAIGLFLQAKISYGIGKAANAGGVSVSGLEMAQNASMHPWSFEVVD
AKLHHIMKEIYKNVSQTAKEFKDPTNFVLGANIAGFRKVASAMIAQGV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=337, Percent_Identity=30.2670623145401, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI4885281, Length=337, Percent_Identity=29.9703264094956, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1788059, Length=449, Percent_Identity=57.6837416481069, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544676, Length=338, Percent_Identity=27.810650887574, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=48.2378854625551, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=48.2300884955752, Blast_Score=358, Evalue=1e-99,
Organism=Drosophila melanogaster, GI45549226, Length=359, Percent_Identity=28.9693593314763, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45553475, Length=328, Percent_Identity=30.7926829268293, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24649460, Length=324, Percent_Identity=28.7037037037037, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24649283, Length=317, Percent_Identity=29.0220820189274, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49430; Mature: 49430

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYEKHAILERLIEPEREIFFR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHEEE
VCWLDDNHQIQVNRGCRVEFNSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAM
EEEECCCCEEEECCCCEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEE
GGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDVPAGDIGVGEREIGYLFGQYK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
KLVNRFEGVLTGKGLTYGGSLCRKEATGYGCVYFAEEMLQERNSSLEGKVCSVSGSGNVA
HHHHHHHHHEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCEE
IYTIEKLLQIGAKPVTASDSNGMIYDKDGIDLELLKEIKEIRRGRIKEYALEKKSAKYTP
EEEHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TENYPKGGNAVWHVPCFAAFPSATENELSVLDAKTLLSNGCKCVAEGANMPSSNEAIGLF
CCCCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEE
LQAKISYGIGKAANAGGVSVSGLEMAQNASMHPWSFEVVDAKLHHIMKEIYKNVSQTAKE
EEEEEHHCCCCCCCCCCCEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKDPTNFVLGANIAGFRKVASAMIAQGV
HCCCCCEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYIEKILQSLQKKYPYQKEFHQAVYEAITSLKPLLDSDKSYEKHAILERLIEPEREIFFR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHEEE
VCWLDDNHQIQVNRGCRVEFNSAIGPYKGGLRFHPSVNESVIKFLGFEQVLKNSLTTLAM
EEEECCCCEEEECCCCEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEE
GGAKGGSDFDPKGKSEHEIMRFCQAFMNELYRHIGATTDVPAGDIGVGEREIGYLFGQYK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
KLVNRFEGVLTGKGLTYGGSLCRKEATGYGCVYFAEEMLQERNSSLEGKVCSVSGSGNVA
HHHHHHHHHEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCEE
IYTIEKLLQIGAKPVTASDSNGMIYDKDGIDLELLKEIKEIRRGRIKEYALEKKSAKYTP
EEEHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TENYPKGGNAVWHVPCFAAFPSATENELSVLDAKTLLSNGCKCVAEGANMPSSNEAIGLF
CCCCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEE
LQAKISYGIGKAANAGGVSVSGLEMAQNASMHPWSFEVVDAKLHHIMKEIYKNVSQTAKE
EEEEEHHCCCCCCCCCCCEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKDPTNFVLGANIAGFRKVASAMIAQGV
HCCCCCEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]