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The map label for this gene is glcD [H]

Identifier: 108562908

GI number: 108562908

Start: 500915

End: 502294

Strand: Direct

Name: glcD [H]

Synonym: HPAG1_0483

Alternate gene names: 108562908

Gene position: 500915-502294 (Clockwise)

Preceding gene: 108562907

Following gene: 108562909

Centisome position: 31.38

GC content: 42.39

Gene sequence:

>1380_bases
ATGTTAGAACAACAGCACATTAAATATTTTAAAAACCTAGTGGGGGGAGAGGATTTTTTCACTGATTTAGCGCATTTGAA
CGCTTATTGCTATGACGCTACTAAAGAAAGGCATTTGCCTAGCGGTGTGATTTTCCCTAGAAACGAGCGAGAAATCAGTC
AGATTTTAAAATATTGCAACGAGCACCGCCTTATCGTTGTGCCTAGGGGGGCTGGGAGCGGTTTTACAGGGGGGGCGTTG
AGCGTGAGCGGGGGGCTAGTTTTAAGCGTGGAAAAACATTTGGATAAGATTTTAGAGATTGACACTAAAAATTTAATCGC
TAGAGTTGAGCCGGGCGTGATTAACAAGCATTTCCAAAACGAAGTGGAAAAATTGAATCTCTTCTACCCCCCAGATCCAG
CGAGTGAAAATCAAAGCACTTTAGGGGGGAATGTCGCTGAAAATGCCGGTGGCATGCGCGCGGCTAAATACGGCATCACT
AAAGACTATGTCATGGCTTTGAGAGTGGTTTTAGCGAATGGTGAAATCATAAGGGCAGGCAAAAAAACGATCAAAGATGT
CGCCGGCTTTAATGTTGCAGGGCTGATGATCGCTAGTGAGGGGTGTTTGGGCGTGATTTCTGAGATCACCTTAAAGCTTT
TAGCCAAACCGCCTCTAAAACAGAGCGCGATGGGGGTTTTTAACCATATTGAAGACGCCATGAACGCTGTTTATAAGACA
ATGAGCAGCGGCGTTACGCCTGTAGCGATGGAATTTTTGGATAATTTGAGTATCAAAGCGGTTGAAGAGAGATTTTCTAA
AGGCTTACCCAAAGACGCTGGAGCGATACTCATCACTCAAGTGGATGGCGTGGTAAAAGAGCAGATTGCATGGCAACTCA
ATGAAATAGAAAAGCATTTTAAAGCCAATGGGTGCGTGGATTTTAAGATCGCTCAAAACGAACAAGAAGAGCAAGATTTA
TGGTTTTCAAGGCGTAACGCTTCTCAAAGTATTAGCGTTTATGGTAAAAAGAAATTGAATGAAGATGTAACCGTTCCTAG
AGCGAGTTTGCCGAGTTTGTTGCAAGAAGTCACTAAAATAAGCCAAAAATACGGCTTTAAAATCCCTTGCTTTGGGCATA
CGGGCGATGGTAATGTGCATGTGAATATCATGCTAGAAGATCCTAAAAAAGATTTAGAAAAAGGCCATAAGGCTATGGAA
GAGATTTTTCAGGCCGCTATTAGTTTGGAGGGGACTTTAAGCGGGGAGCATGGCATAGGCTTGTCTAAAGCCAAATTCAT
GCCCTTAGCGTTCAATCATAGTGAAATGGAGCTTTTTAGGAACATTAAAAAAGCCCTTGATCCTAATAATATTTTAAACC
CTTTTAAAATGGGGCTATAA

Upstream 100 bases:

>100_bases
AGAGCGAGAGAATTTGAACAAAGAGCGAGAGAGCATCAAGAAAGAGATGAAAAAGAGCTTGAAGAGCGAAGAAAGGCGCT
AGAAGCGGGTAAAAAATAAC

Downstream 100 bases:

>100_bases
AATTTAAAAGCAAGGAAAGCGCATGAAAATCGGTGTTTATGGAGCGAGCGGTCGTATAGGGAAACTGCTTTTAGAAGAAT
TAAAAGGGGGGTATAAGGGA

Product: glycolate oxidase subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLEQQHIKYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPRNEREISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGIT
KDYVMALRVVLANGEIIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVDFKIAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVTKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAME
EIFQAAISLEGTLSGEHGIGLSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL

Sequences:

>Translated_459_residues
MLEQQHIKYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPRNEREISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGIT
KDYVMALRVVLANGEIIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVDFKIAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVTKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAME
EIFQAAISLEGTLSGEHGIGLSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL
>Mature_459_residues
MLEQQHIKYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPRNEREISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGIT
KDYVMALRVVLANGEIIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVDFKIAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVTKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAME
EIFQAAISLEGTLSGEHGIGLSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=464, Percent_Identity=30.8189655172414, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI37595754, Length=487, Percent_Identity=29.1581108829569, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI119964728, Length=464, Percent_Identity=27.3706896551724, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI4501993, Length=255, Percent_Identity=23.921568627451, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789351, Length=447, Percent_Identity=35.1230425055928, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1787977, Length=504, Percent_Identity=23.8095238095238, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI48994907, Length=487, Percent_Identity=23.2032854209446, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI48994914, Length=134, Percent_Identity=31.3432835820896, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=425, Percent_Identity=31.2941176470588, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI71992381, Length=425, Percent_Identity=29.8823529411765, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI71992392, Length=385, Percent_Identity=29.8701298701299, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17534361, Length=463, Percent_Identity=27.645788336933, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17556096, Length=456, Percent_Identity=25.4385964912281, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320027, Length=427, Percent_Identity=28.3372365339578, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6320023, Length=479, Percent_Identity=28.1837160751566, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6320764, Length=477, Percent_Identity=27.0440251572327, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI18921117, Length=465, Percent_Identity=26.4516129032258, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24639277, Length=465, Percent_Identity=26.4516129032258, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24639275, Length=465, Percent_Identity=26.4516129032258, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24653753, Length=467, Percent_Identity=23.5546038543897, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50596; Mature: 50596

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQQHIKYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPRNEREISQILKYCN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHC
EHRLIVVPRGAGSGFTGGALSVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQN
CCCEEEEECCCCCCCCCCEEEECCCEEEEHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
EVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGITKDYVMALRVVLANGEIIRAG
HHHHEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEECH
KKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHF
HHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
KANGCVDFKIAQNEQEEQDLWFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVTKI
CCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHH
SQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAMEEIFQAAISLEGTLSGEHGIG
HHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MLEQQHIKYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPRNEREISQILKYCN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHC
EHRLIVVPRGAGSGFTGGALSVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQN
CCCEEEEECCCCCCCCCCEEEECCCEEEEHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
EVEKLNLFYPPDPASENQSTLGGNVAENAGGMRAAKYGITKDYVMALRVVLANGEIIRAG
HHHHEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEECH
KKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPPLKQSAMGVFNHIEDAMNAVYKT
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHF
HHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
KANGCVDFKIAQNEQEEQDLWFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVTKI
CCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHH
SQKYGFKIPCFGHTGDGNVHVNIMLEDPKKDLEKGHKAMEEIFQAAISLEGTLSGEHGIG
HHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]