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The map label for this gene is fliA [H]

Identifier: 108562840

GI number: 108562840

Start: 424937

End: 425704

Strand: Direct

Name: fliA [H]

Synonym: HPAG1_0415

Alternate gene names: 108562840

Gene position: 424937-425704 (Clockwise)

Preceding gene: 108562838

Following gene: 108562841

Centisome position: 26.62

GC content: 39.32

Gene sequence:

>768_bases
ATGATTTTGATGATGGAAAATAGAATGCCCAAAGAAATTCAAAAACATGAAACCAGCGAAAAAAATATAGAAAAGGTTTT
GAACGCCTACGATGAGCAGCAACACCACCATCAAGACGCGCTCGCTATCCAGTATTTACCGGCCGTGCGCGCTATGGCGT
TTCGTTTAAAAGAGCGCTTGCCCAGCTCTATTGATTTTAACGATCTGGTTTCTATTGGCACTGAAGAATTGATTAAATTA
GCCAGGCGTTATGAGAGCGCGTTAAACGATTCTTTTTGGGGGTATGCTAAAACTCGTGTCAATGGGGCGATGCTAGATTA
TTTGCGCTCTTTAGATGTGATTTCTCGCTCCAACAGAAAGCTGATTAAAAGCATTGATATTGAGATCACCAAGCACCTTA
ATGAGCATGGGAAAGAGCCTAGCGATGCGTATTTAGCGCAAACTTTAGGCGAAAATATTGAAAAAATTAAAGAAGCCAAA
ACGGCTTCAGATATTTATGCGTTAGTGCCAATAGATGAACAATTCAATGCGATTGAGCAAGATGAAATCACTAAAAAGAT
TGAAGCAGAGGAGTTGTTAGAGCATGTCCAAAAAGTATTGAATCAAATGAGCGAAAGAGAGCAAATCCTTATCCAGCTTT
ATTACTTTGAAGAGTTGAATTTGAGCGAGATCAAAGAGATTTTAGGCATCACTGAATCGCGCATTTCTCAAATCATTAAA
GAAGTGATTAAAAAGGTGCGTAAATCCTTAGGAGTGGATCATGGCTGA

Upstream 100 bases:

>100_bases
TTTGACAAAAAAGGCACTGAAGAGAGCTTGCTTCGTTTTAACCATGAGTTTAAGAACAGAGAAAAAGAAGTGGCTAGTAT
TTTAGAATACATTAGAAGTT

Downstream 100 bases:

>100_bases
TATTTTAAGCCAAGAAGAAATTGATGCGCTTTTAGAAGTCGTTGATGAAAATGTGGATATTCAAAATGTCCAAAAAAAGG
ATATTATCCCGCAGCGCAGC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MILMMENRMPKEIQKHETSEKNIEKVLNAYDEQQHHHQDALAIQYLPAVRAMAFRLKERLPSSIDFNDLVSIGTEELIKL
ARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRKLIKSIDIEITKHLNEHGKEPSDAYLAQTLGENIEKIKEAK
TASDIYALVPIDEQFNAIEQDEITKKIEAEELLEHVQKVLNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIK
EVIKKVRKSLGVDHG

Sequences:

>Translated_255_residues
MILMMENRMPKEIQKHETSEKNIEKVLNAYDEQQHHHQDALAIQYLPAVRAMAFRLKERLPSSIDFNDLVSIGTEELIKL
ARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRKLIKSIDIEITKHLNEHGKEPSDAYLAQTLGENIEKIKEAK
TASDIYALVPIDEQFNAIEQDEITKKIEAEELLEHVQKVLNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIK
EVIKKVRKSLGVDHG
>Mature_255_residues
MILMMENRMPKEIQKHETSEKNIEKVLNAYDEQQHHHQDALAIQYLPAVRAMAFRLKERLPSSIDFNDLVSIGTEELIKL
ARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRKLIKSIDIEITKHLNEHGKEPSDAYLAQTLGENIEKIKEAK
TASDIYALVPIDEQFNAIEQDEITKKIEAEELLEHVQKVLNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIK
EVIKKVRKSLGVDHG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=223, Percent_Identity=33.1838565022422, Blast_Score=114, Evalue=7e-27,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29562; Mature: 29562

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMMENRMPKEIQKHETSEKNIEKVLNAYDEQQHHHQDALAIQYLPAVRAMAFRLKERL
CEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PSSIDFNDLVSIGTEELIKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRK
CCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHH
LIKSIDIEITKHLNEHGKEPSDAYLAQTLGENIEKIKEAKTASDIYALVPIDEQFNAIEQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCCH
DEITKKIEAEELLEHVQKVLNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
EVIKKVRKSLGVDHG
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MILMMENRMPKEIQKHETSEKNIEKVLNAYDEQQHHHQDALAIQYLPAVRAMAFRLKERL
CEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PSSIDFNDLVSIGTEELIKLARRYESALNDSFWGYAKTRVNGAMLDYLRSLDVISRSNRK
CCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHH
LIKSIDIEITKHLNEHGKEPSDAYLAQTLGENIEKIKEAKTASDIYALVPIDEQFNAIEQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCCH
DEITKKIEAEELLEHVQKVLNQMSEREQILIQLYYFEELNLSEIKEILGITESRISQIIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
EVIKKVRKSLGVDHG
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]