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The map label for this gene is prfA

Identifier: 103488191

GI number: 103488191

Start: 2861337

End: 2862404

Strand: Reverse

Name: prfA

Synonym: Sala_2714

Alternate gene names: 103488191

Gene position: 2862404-2861337 (Counterclockwise)

Preceding gene: 103488192

Following gene: 103488190

Centisome position: 85.57

GC content: 66.95

Gene sequence:

>1068_bases
ATGACCTCCGTTTCCGCCCGCCAGATCGACGCCATCATCGAACGCCACGCCGCGCTCCAGGCGCGCATGGCGACGGGCGA
TATGGCGCCCGCCGATTTTGTCGCGGCGTCGAAGGAGTTTGCCGAGCTGGAGCCCGTCGCGACCGCGGCGCGTGAAGTCG
TGCGGTTGCGCGAAGAACTCGTCGCGCTCAACGAAATGCTCGCCGATCCGGAGATGAAGGCGATGGCGGTCGAGGAAATC
GCGGCGGTCGAGGCGGCGCTGCCCGCCGCCGAGCACGACCTTGCGCTCCAGCTGCTCCCCAAGGATGTCGCTGACTCGCG
CGCGGCGATGCTTGAAATCCGCGCCGGCACTGGCGGCGACGAGGCGGCGCTGTTCGCGGGCGATTTGCTCCGCATGTACA
GCCGCTATGCCGATACGCAGGGGTGGAAGGTCGAAATCATCTCGGCGAACGAGGCCGAGGTCGGCGGCTACAAGGAAGTC
GTCGCGTCGGTCAGCGGGCAGGGCGTGTTCGCCAAGCTGAAGTTCGAAAGCGGCGTGCATCGGGTGCAGCGCGTCCCCGT
CACCGAAAGCGGCGGGCGCATCCACACCAGCGCCGCGACCGTCGCGGTGCTGCCCGAGGCCGAGGAGGTCGACGTCGCGA
TCAACGACAGCGACCTGAAGATCGACATCTATCGCGCGAGCGGAGCGGGTGGCCAGCACGTCAACACGACCGATTCGGCG
ATCCGCATCACGCACATCCCGACTGGCCTTGTCGTCATCCAGCAGGACCAGCGCAGCCAGCACAAGAATCGCGCGAAGGC
GATGCAGGTGCTGCGCGCGCGGCTTTATGATCTGGAGCGCGAGAAGCTTCACAGCGCCGAGGCGTCGGCGCGCAAGTCGA
TGGTCGGATCGGGCGACCGCTCCGAACGCATCCGCACCTATAATTTCCCGCAGGGGCGCGTGACCGACCACCGCATCAAC
CTGACGCTCCACCGCCTGCCGGAGATATTGGAAGGCGCGATGGACGAACTCATCGACGCGCTGATCGCCGAGGATCAGGC
GCAGCGACTGGCGGGGCTGGGTGAGTGA

Upstream 100 bases:

>100_bases
CCATCGGCCTTGCGCAAGGCCGATGGCCCCCACCCGCTGCAACTAGGCAGCAAGCTGCCAAGTTTCGCTGCCCTCCCCTG
AAGGGGAGGGCGTGTTTGGC

Downstream 100 bases:

>100_bases
CCGACCGAAACCCGTTCGCATCGAGCGAAGTCGAGATGCCCATCGGCTTGGCGTGATGCCGAAGGGCGTCTCGACTTCGC
TCGACACGAACGGGACTAGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTSVSARQIDAIIERHAALQARMATGDMAPADFVAASKEFAELEPVATAAREVVRLREELVALNEMLADPEMKAMAVEEI
AAVEAALPAAEHDLALQLLPKDVADSRAAMLEIRAGTGGDEAALFAGDLLRMYSRYADTQGWKVEIISANEAEVGGYKEV
VASVSGQGVFAKLKFESGVHRVQRVPVTESGGRIHTSAATVAVLPEAEEVDVAINDSDLKIDIYRASGAGGQHVNTTDSA
IRITHIPTGLVVIQQDQRSQHKNRAKAMQVLRARLYDLEREKLHSAEASARKSMVGSGDRSERIRTYNFPQGRVTDHRIN
LTLHRLPEILEGAMDELIDALIAEDQAQRLAGLGE

Sequences:

>Translated_355_residues
MTSVSARQIDAIIERHAALQARMATGDMAPADFVAASKEFAELEPVATAAREVVRLREELVALNEMLADPEMKAMAVEEI
AAVEAALPAAEHDLALQLLPKDVADSRAAMLEIRAGTGGDEAALFAGDLLRMYSRYADTQGWKVEIISANEAEVGGYKEV
VASVSGQGVFAKLKFESGVHRVQRVPVTESGGRIHTSAATVAVLPEAEEVDVAINDSDLKIDIYRASGAGGQHVNTTDSA
IRITHIPTGLVVIQQDQRSQHKNRAKAMQVLRARLYDLEREKLHSAEASARKSMVGSGDRSERIRTYNFPQGRVTDHRIN
LTLHRLPEILEGAMDELIDALIAEDQAQRLAGLGE
>Mature_354_residues
TSVSARQIDAIIERHAALQARMATGDMAPADFVAASKEFAELEPVATAAREVVRLREELVALNEMLADPEMKAMAVEEIA
AVEAALPAAEHDLALQLLPKDVADSRAAMLEIRAGTGGDEAALFAGDLLRMYSRYADTQGWKVEIISANEAEVGGYKEVV
ASVSGQGVFAKLKFESGVHRVQRVPVTESGGRIHTSAATVAVLPEAEEVDVAINDSDLKIDIYRASGAGGQHVNTTDSAI
RITHIPTGLVVIQQDQRSQHKNRAKAMQVLRARLYDLEREKLHSAEASARKSMVGSGDRSERIRTYNFPQGRVTDHRINL
TLHRLPEILEGAMDELIDALIAEDQAQRLAGLGE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=275, Percent_Identity=47.6363636363636, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI34577120, Length=321, Percent_Identity=41.1214953271028, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI166795305, Length=178, Percent_Identity=46.0674157303371, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=57.3863636363636, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=39.7058823529412, Blast_Score=215, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=347, Percent_Identity=37.463976945245, Blast_Score=212, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321295, Length=327, Percent_Identity=45.565749235474, Blast_Score=265, Evalue=9e-72,
Organism=Drosophila melanogaster, GI19921226, Length=320, Percent_Identity=42.5, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SPHAL (Q1GPK3)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617752.1
- ProteinModelPortal:   Q1GPK3
- SMR:   Q1GPK3
- GeneID:   4082415
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2714
- NMPDR:   fig|317655.9.peg.2580
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- BioCyc:   SALA317655:SALA_2714-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38474; Mature: 38343

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVSARQIDAIIERHAALQARMATGDMAPADFVAASKEFAELEPVATAAREVVRLREEL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALNEMLADPEMKAMAVEEIAAVEAALPAAEHDLALQLLPKDVADSRAAMLEIRAGTGGD
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCEEEEEEECCCCCC
EAALFAGDLLRMYSRYADTQGWKVEIISANEAEVGGYKEVVASVSGQGVFAKLKFESGVH
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEHHHHH
RVQRVPVTESGGRIHTSAATVAVLPEAEEVDVAINDSDLKIDIYRASGAGGQHVNTTDSA
HHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCE
IRITHIPTGLVVIQQDQRSQHKNRAKAMQVLRARLYDLEREKLHSAEASARKSMVGSGDR
EEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SERIRTYNFPQGRVTDHRINLTLHRLPEILEGAMDELIDALIAEDQAQRLAGLGE
CCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSVSARQIDAIIERHAALQARMATGDMAPADFVAASKEFAELEPVATAAREVVRLREEL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALNEMLADPEMKAMAVEEIAAVEAALPAAEHDLALQLLPKDVADSRAAMLEIRAGTGGD
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCEEEEEEECCCCCC
EAALFAGDLLRMYSRYADTQGWKVEIISANEAEVGGYKEVVASVSGQGVFAKLKFESGVH
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEHHHHH
RVQRVPVTESGGRIHTSAATVAVLPEAEEVDVAINDSDLKIDIYRASGAGGQHVNTTDSA
HHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCE
IRITHIPTGLVVIQQDQRSQHKNRAKAMQVLRARLYDLEREKLHSAEASARKSMVGSGDR
EEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SERIRTYNFPQGRVTDHRINLTLHRLPEILEGAMDELIDALIAEDQAQRLAGLGE
CCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA