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The map label for this gene is hemK [H]

Identifier: 103488190

GI number: 103488190

Start: 2860411

End: 2861235

Strand: Reverse

Name: hemK [H]

Synonym: Sala_2713

Alternate gene names: 103488190

Gene position: 2861235-2860411 (Counterclockwise)

Preceding gene: 103488191

Following gene: 103488189

Centisome position: 85.53

GC content: 69.21

Gene sequence:

>825_bases
GTGGGTGAGGTAGCGCAGAGGCTGCGCGATGCGGCGCAGCGGCTCGCCGCGGTGTCGGACACGCCGCGGCTCGATGCCGA
ACTGCTGATGGCCGACGCGCTGGGGATCGAGCGGCAGGCGCTGCTGCTCGACCCGGCGCGTTACGATGTCCCGGCGGACT
TCGACGCGCTGGTGGAACGGCGCATGGCGCACGAGCCCGTCGCCTATATCCTCGGCTACCGCGATTTCTGGACGATCCGG
CTGGCGGTCGGTCCCGGCGCGTTGATCCCGCGACCCGACAGCGAGACGCTGATCGCGGCGGCGGTGCAGCATTTCGGCGC
GGCGGGGCCGCGGCATATTCTTGACCTCGGCACCGGACCCGGGACGCTGCTGTTTGCGGCCATGAGTGAATGGGCCGATG
CGCGCGGGCTTGGCGTCGATGCCAGCGACGTGGCGCTCGGCTATGCGCGCGCCAATGCGATCGCGCTTGGGCTGGCCGAT
CGTGTCGGGCTGATGCAGGGCGATTGGGCGGCGGGCGTCACCGGCCGGTTCGACCTCATCCTCTGCAACCCTCCCTATAT
CGCAGAGAGCGAAGCGTTGATGCCCGATGTCGCCGATCACGAGCCTGCCGGTGCGCTGTTCGCCGGCGCCGATGGCCTCG
CCGATTATCGCCGTATCATCCCCGACCTGCCGCACCTGCTTGCGCCCGGTGGCATCGCCATCCTTGAAATCGGCCACACG
CAACGCATATCGGTGAGCGAGATGGCCGACGCGGCAGGGTTCGCTGTCGCCTGTCGCCAGGATCTGGCTGGCCGCGACCG
GGCGCTTTTGCTGACGTTCGCCTGA

Upstream 100 bases:

>100_bases
CGACCGAAACCCGTTCGCATCGAGCGAAGTCGAGATGCCCATCGGCTTGGCGTGATGCCGAAGGGCGTCTCGACTTCGCT
CGACACGAACGGGACTAGAG

Downstream 100 bases:

>100_bases
GTCCCGCACAAGAAATTGCTTGGTTTTGGCGGCAAAGCGCGGTAGGGGCGGCTTACAGGCCTGATCAGGGTCCCTTTGGC
GGTGCGGCGCACCGATACTG

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MGEVAQRLRDAAQRLAAVSDTPRLDAELLMADALGIERQALLLDPARYDVPADFDALVERRMAHEPVAYILGYRDFWTIR
LAVGPGALIPRPDSETLIAAAVQHFGAAGPRHILDLGTGPGTLLFAAMSEWADARGLGVDASDVALGYARANAIALGLAD
RVGLMQGDWAAGVTGRFDLILCNPPYIAESEALMPDVADHEPAGALFAGADGLADYRRIIPDLPHLLAPGGIAILEIGHT
QRISVSEMADAAGFAVACRQDLAGRDRALLLTFA

Sequences:

>Translated_274_residues
MGEVAQRLRDAAQRLAAVSDTPRLDAELLMADALGIERQALLLDPARYDVPADFDALVERRMAHEPVAYILGYRDFWTIR
LAVGPGALIPRPDSETLIAAAVQHFGAAGPRHILDLGTGPGTLLFAAMSEWADARGLGVDASDVALGYARANAIALGLAD
RVGLMQGDWAAGVTGRFDLILCNPPYIAESEALMPDVADHEPAGALFAGADGLADYRRIIPDLPHLLAPGGIAILEIGHT
QRISVSEMADAAGFAVACRQDLAGRDRALLLTFA
>Mature_273_residues
GEVAQRLRDAAQRLAAVSDTPRLDAELLMADALGIERQALLLDPARYDVPADFDALVERRMAHEPVAYILGYRDFWTIRL
AVGPGALIPRPDSETLIAAAVQHFGAAGPRHILDLGTGPGTLLFAAMSEWADARGLGVDASDVALGYARANAIALGLADR
VGLMQGDWAAGVTGRFDLILCNPPYIAESEALMPDVADHEPAGALFAGADGLADYRRIIPDLPHLLAPGGIAILEIGHTQ
RISVSEMADAAGFAVACRQDLAGRDRALLLTFA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=271, Percent_Identity=29.8892988929889, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=35.6363636363636, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI87082085, Length=190, Percent_Identity=34.2105263157895, Blast_Score=83, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582226, Length=208, Percent_Identity=31.25, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28979; Mature: 28848

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVAQRLRDAAQRLAAVSDTPRLDAELLMADALGIERQALLLDPARYDVPADFDALVER
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCHHCCCCCCHHHHHHH
RMAHEPVAYILGYRDFWTIRLAVGPGALIPRPDSETLIAAAVQHFGAAGPRHILDLGTGP
HHHCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCC
GTLLFAAMSEWADARGLGVDASDVALGYARANAIALGLADRVGLMQGDWAAGVTGRFDLI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEHHHHCCCCCCCCCCCCCCCEEEE
LCNPPYIAESEALMPDVADHEPAGALFAGADGLADYRRIIPDLPHLLAPGGIAILEIGHT
EECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHCCCCEEEEEECCC
QRISVSEMADAAGFAVACRQDLAGRDRALLLTFA
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
GEVAQRLRDAAQRLAAVSDTPRLDAELLMADALGIERQALLLDPARYDVPADFDALVER
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCHHCCCCCCHHHHHHH
RMAHEPVAYILGYRDFWTIRLAVGPGALIPRPDSETLIAAAVQHFGAAGPRHILDLGTGP
HHHCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCC
GTLLFAAMSEWADARGLGVDASDVALGYARANAIALGLADRVGLMQGDWAAGVTGRFDLI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEHHHHCCCCCCCCCCCCCCCEEEE
LCNPPYIAESEALMPDVADHEPAGALFAGADGLADYRRIIPDLPHLLAPGGIAILEIGHT
EECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHCCCCEEEEEECCC
QRISVSEMADAAGFAVACRQDLAGRDRALLLTFA
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]