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The map label for this gene is ispG [H]

Identifier: 103487332

GI number: 103487332

Start: 1943525

End: 1944676

Strand: Direct

Name: ispG [H]

Synonym: Sala_1848

Alternate gene names: 103487332

Gene position: 1943525-1944676 (Clockwise)

Preceding gene: 103487330

Following gene: 103487333

Centisome position: 58.1

GC content: 67.62

Gene sequence:

>1152_bases
ATGAGCGATCATAATCCCGGCCTGCGCCCCTGGCGCGACATTGCACGGCGGACGAGCCGCCAGATCATGGTCGGAAACGT
CCCCGTCGGCGGCGGCGCGCCGATCAGCGTGCAGACAATGACCAACACGCTGACGAGCGATCCGGCGCAGACGATCGACC
AGATCCGCCGCTGCGAGGACGCCGGCGCCGACCTGATCCGCGTCTCGTGCCCCGACACCGATTCGACCGCGGCGCTCGGC
AAGATCGTCCGCGCGGCGCGCATTCCGATCATCGCCGACATCCATTTCCACTATAAGCGCGCATTGGAAGCCGCCGACGC
CGGCGCCGCCTGCCTGCGCATCAACCCCGGCAATATCGGCTCGTCGGAACGCGTCGGCGAGGTCGTGCGCGCCGCCAAGG
CGAACGGCTGCGCGATCCGCATCGGCGTCAACGCCGGCAGCCTCGAGAAGGACCTGCTCGAAAAATATGGCGAGCCCTGC
CCCGAGGCGCTCGTCGAAAGCGCGCTCGACCATATCAAGCTGCTGCAGGACCATGATTTCCACGACTACAAGGTCGCGGT
GAAGGCGAGCGACGTCTTCCTCGCGGTCGCCGCCTATGCGCAGCTCGCCGACGCGGTCGACTGCCCGCTGCACCTCGGCA
TCACCGAGGCGGGCGGGCTGATCGGTGGCACGGTGAAAAGCGCGCTCGGCATCGGCAACCTGCTCTGGGCCGGGATCGGC
GACACGATCCGCGTTTCGCTTTCGGCGGAACCCGAAGAGGAAGTACGCGTCGGCTACGAGATACTGAAATCGCTGGGCCT
CAGAACCCGCGGCGTGCGCGTCGTCTCCTGCCCCAGCTGCGCACGACAGGGTTTCGACGTCATCCGCACCGTCCAGGCGC
TCGAGGACGCGCTCGGCCACATCAAGACGCCGATGTCGCTCTCGGTCCTCGGCTGCGTCGTCAACGGCCCCGGCGAAGCG
CGCGAGACCGACATCGGCATCACCGGCGGCGGCAACGGCAGGCATATGGTCTATCTCTCGGGCGTCACCGACCACCATGT
CGAGGACGCCGACATGATCGCGCACATCGTCAGGCTCGTCGAAGCCAAGGCGGCCGAGATCGAGGCGGGCAACGCGGTGA
GCATGGACGTCGCGCACGGCAAGGCGGCGTAG

Upstream 100 bases:

>100_bases
TGTCTTTCCAGCAACAATCCGTCCCCCACGCGCCGGGCTTTGCCAAGCCGCGCGCAGCCAGCTAAAGAGCGCGCATATCC
CCCTCTGCGAGCCATTAGCA

Downstream 100 bases:

>100_bases
GGCCGATCATCCCTGCGATGCCGTCCGTTTTGGGGTGCGGAGCTGCCTTTCCCCTTTCCCCTCTCCCGTCGGGAGAGGGA
TACGCAGGCTTGGTTGCGTA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSDHNPGLRPWRDIARRTSRQIMVGNVPVGGGAPISVQTMTNTLTSDPAQTIDQIRRCEDAGADLIRVSCPDTDSTAALG
KIVRAARIPIIADIHFHYKRALEAADAGAACLRINPGNIGSSERVGEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPC
PEALVESALDHIKLLQDHDFHDYKVAVKASDVFLAVAAYAQLADAVDCPLHLGITEAGGLIGGTVKSALGIGNLLWAGIG
DTIRVSLSAEPEEEVRVGYEILKSLGLRTRGVRVVSCPSCARQGFDVIRTVQALEDALGHIKTPMSLSVLGCVVNGPGEA
RETDIGITGGGNGRHMVYLSGVTDHHVEDADMIAHIVRLVEAKAAEIEAGNAVSMDVAHGKAA

Sequences:

>Translated_383_residues
MSDHNPGLRPWRDIARRTSRQIMVGNVPVGGGAPISVQTMTNTLTSDPAQTIDQIRRCEDAGADLIRVSCPDTDSTAALG
KIVRAARIPIIADIHFHYKRALEAADAGAACLRINPGNIGSSERVGEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPC
PEALVESALDHIKLLQDHDFHDYKVAVKASDVFLAVAAYAQLADAVDCPLHLGITEAGGLIGGTVKSALGIGNLLWAGIG
DTIRVSLSAEPEEEVRVGYEILKSLGLRTRGVRVVSCPSCARQGFDVIRTVQALEDALGHIKTPMSLSVLGCVVNGPGEA
RETDIGITGGGNGRHMVYLSGVTDHHVEDADMIAHIVRLVEAKAAEIEAGNAVSMDVAHGKAA
>Mature_382_residues
SDHNPGLRPWRDIARRTSRQIMVGNVPVGGGAPISVQTMTNTLTSDPAQTIDQIRRCEDAGADLIRVSCPDTDSTAALGK
IVRAARIPIIADIHFHYKRALEAADAGAACLRINPGNIGSSERVGEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCP
EALVESALDHIKLLQDHDFHDYKVAVKASDVFLAVAAYAQLADAVDCPLHLGITEAGGLIGGTVKSALGIGNLLWAGIGD
TIRVSLSAEPEEEVRVGYEILKSLGLRTRGVRVVSCPSCARQGFDVIRTVQALEDALGHIKTPMSLSVLGCVVNGPGEAR
ETDIGITGGGNGRHMVYLSGVTDHHVEDADMIAHIVRLVEAKAAEIEAGNAVSMDVAHGKAA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=362, Percent_Identity=53.8674033149171, Blast_Score=393, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588
- InterPro:   IPR011060 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40300; Mature: 40169

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHNPGLRPWRDIARRTSRQIMVGNVPVGGGAPISVQTMTNTLTSDPAQTIDQIRRCED
CCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
AGADLIRVSCPDTDSTAALGKIVRAARIPIIADIHFHYKRALEAADAGAACLRINPGNIG
CCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCC
SSERVGEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEALVESALDHIKLLQDHDF
CHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
HDYKVAVKASDVFLAVAAYAQLADAVDCPLHLGITEAGGLIGGTVKSALGIGNLLWAGIG
CCEEEEEEECHHHHHHHHHHHHHHHHCCCEEECCHHCCCCHHHHHHHHHHHHHHHHHCCC
DTIRVSLSAEPEEEVRVGYEILKSLGLRTRGVRVVSCPSCARQGFDVIRTVQALEDALGH
CEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
IKTPMSLSVLGCVVNGPGEARETDIGITGGGNGRHMVYLSGVTDHHVEDADMIAHIVRLV
CCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
EAKAAEIEAGNAVSMDVAHGKAA
HHHHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SDHNPGLRPWRDIARRTSRQIMVGNVPVGGGAPISVQTMTNTLTSDPAQTIDQIRRCED
CCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
AGADLIRVSCPDTDSTAALGKIVRAARIPIIADIHFHYKRALEAADAGAACLRINPGNIG
CCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCC
SSERVGEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEALVESALDHIKLLQDHDF
CHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
HDYKVAVKASDVFLAVAAYAQLADAVDCPLHLGITEAGGLIGGTVKSALGIGNLLWAGIG
CCEEEEEEECHHHHHHHHHHHHHHHHCCCEEECCHHCCCCHHHHHHHHHHHHHHHHHCCC
DTIRVSLSAEPEEEVRVGYEILKSLGLRTRGVRVVSCPSCARQGFDVIRTVQALEDALGH
CEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
IKTPMSLSVLGCVVNGPGEARETDIGITGGGNGRHMVYLSGVTDHHVEDADMIAHIVRLV
CCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
EAKAAEIEAGNAVSMDVAHGKAA
HHHHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA