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The map label for this gene is nusA [H]

Identifier: 103486103

GI number: 103486103

Start: 619846

End: 621492

Strand: Direct

Name: nusA [H]

Synonym: Sala_0610

Alternate gene names: 103486103

Gene position: 619846-621492 (Clockwise)

Preceding gene: 103486102

Following gene: 103486104

Centisome position: 18.53

GC content: 64.85

Gene sequence:

>1647_bases
ATGGCCACTGCCATTTCCGCCAACAAGGCCGAACTGCTCGCGATCGCCAACAGCGTCGCCAGCGAGAAGATGATCGACAA
GGCTATCGTCATCGAGGCGATCGAGGAAGCGATCCAGCGTGCCGCGCGCGCCCGCTATGGCGCCGAGAACGACATTCGCG
CCAAGCTCGACCCGCAGACCGGCGACCTGCGCCTGTGGCGCGTCGTCGAAGTGGTCGAGACGGTCGAGGATTATTTCAAG
CAGGTCGACCTCGCCGCCGGGCAAAAGTTGCAGAAGGACGCCAGGATCGGCGACTTCATCGTCGATCCGCTGCCCGCCGT
CGACCTTGGCCGCATCGACGCGCAGTCGGCGAAGCAGGTGATCTTCCAGAAGGTTCGCGAAGCCGACCGGGAACGCCAGT
ATCAGGAGTTCAAGGATCGCGCCGGAGAGATTATCACCGGCGTCGTGAAATCGGTCGAGTTCGGCCATATCGTCGTCAAT
CTGGGCCGCGCCGAGGGCGTGATCCGCCGCGACCAGCAAATCCCGCGCGAATTGATGCGCGTCGGCGACCGCGTCCGCGC
GCTGATCCTGTCGGTGCGCAGCGAAACGCGCGGGCCGCAGATTTTCCTCTCGCGCGCGCATCCCGACTTCATGAAAAAGC
TGTTCGCGCAGGAGGTGCCCGAAATCTATGATGGCATCATCGAGATCAAGGCTGCCGCGCGCGATCCGGGCAGCCGGGCG
AAGATCGGCGTGATCAGCTATGACAGCAGCATCGACCCCGTCGGCGCCTGTGTGGGCATGAAGGGCAGCCGCGTTCAGGC
GGTCGTTCAGGAATTGCAGGGCGAAAAGATCGACATCATTCCCTGGTCGGAGGACACGGCGACCTTCGTGGTCAATGCGC
TTCAGCCGGCCACGGTGCAGCGCGTCGTCATCGACGAGGATGATGGTCGCATCGAGGTCGTCGTCCCCGACGATCAGCTC
AGCCTCGCCATCGGTCGCCGCGGCCAGAACGTCCGTCTTGCCAGCCAGCTGACCGGCAAGCAGATCGATATCATGACCGA
GGCGGATGCGAGCGAGAAGCGGCAGCGCGAATTCGTCGAACGCTCGACGATGTTCCAGGAGGAACTCGACGTCGACGAAA
CACTGGCACAGCTCCTCGTCGCCGAAGGCTTCAGCGAGCTCGAGGAAGTCGCCTATGTGCCGCTCGACGAACTGGCAAGC
ATCGAGGGTTTCGACGAGGAACTGGCGCAGGAACTGCAGAGTCGCGCTGCCGAGGGGCTGGAGCGTCGCGAGGAGGCGGC
GCGCGCCGAGCGTCGCGAACTGGGCGTCGAGGATGCACTCGCCGACATCCCGCACCTGACCGAAGCGATGCTTGTCACGC
TCGGCAAGGCCGGAATCAGGACGCTCGACGATCTTGCAGACCTTGCGACCGACGAACTGATCGCCAAGAAACGAACCGAC
AACCGCCGCGGCCCGGCGCGCAGCGAGCGCGCCGAGGACAAGGGCGGCGTGCTCGGCGAATATGGCCTGAGCGAAGAGCA
GGGCAACGAGATCATCATGGCGGCGCGTGCGCACTGGTTCGACGATGAGGAGTCGAGCGAGCCGGCAACCGCGCCCGCGG
CGGAGCCGCAAAACGGGGAGGCCGCCGATGCGGACCCCGCGCAATGA

Upstream 100 bases:

>100_bases
TCGACACGGCGAAGCTGGTGCTCACCGACAAATTGATTGCCGCAACCGTTCCGCTCTCCGCCGAGGGCGCCGACGAAATG
GAAGAAGAAGGACAGGACTG

Downstream 100 bases:

>100_bases
TCAACTGAGCCAAACCGACCGCGCAAAGGGGCGCGGCCACCATGTGCCCGAGCGGCGCTGCGTCGTCACCGGTGAGGTTT
CGCCGGTAGAGCGGCTCGTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MATAISANKAELLAIANSVASEKMIDKAIVIEAIEEAIQRAARARYGAENDIRAKLDPQTGDLRLWRVVEVVETVEDYFK
QVDLAAGQKLQKDARIGDFIVDPLPAVDLGRIDAQSAKQVIFQKVREADRERQYQEFKDRAGEIITGVVKSVEFGHIVVN
LGRAEGVIRRDQQIPRELMRVGDRVRALILSVRSETRGPQIFLSRAHPDFMKKLFAQEVPEIYDGIIEIKAAARDPGSRA
KIGVISYDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVQRVVIDEDDGRIEVVVPDDQL
SLAIGRRGQNVRLASQLTGKQIDIMTEADASEKRQREFVERSTMFQEELDVDETLAQLLVAEGFSELEEVAYVPLDELAS
IEGFDEELAQELQSRAAEGLERREEAARAERRELGVEDALADIPHLTEAMLVTLGKAGIRTLDDLADLATDELIAKKRTD
NRRGPARSERAEDKGGVLGEYGLSEEQGNEIIMAARAHWFDDEESSEPATAPAAEPQNGEAADADPAQ

Sequences:

>Translated_548_residues
MATAISANKAELLAIANSVASEKMIDKAIVIEAIEEAIQRAARARYGAENDIRAKLDPQTGDLRLWRVVEVVETVEDYFK
QVDLAAGQKLQKDARIGDFIVDPLPAVDLGRIDAQSAKQVIFQKVREADRERQYQEFKDRAGEIITGVVKSVEFGHIVVN
LGRAEGVIRRDQQIPRELMRVGDRVRALILSVRSETRGPQIFLSRAHPDFMKKLFAQEVPEIYDGIIEIKAAARDPGSRA
KIGVISYDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVQRVVIDEDDGRIEVVVPDDQL
SLAIGRRGQNVRLASQLTGKQIDIMTEADASEKRQREFVERSTMFQEELDVDETLAQLLVAEGFSELEEVAYVPLDELAS
IEGFDEELAQELQSRAAEGLERREEAARAERRELGVEDALADIPHLTEAMLVTLGKAGIRTLDDLADLATDELIAKKRTD
NRRGPARSERAEDKGGVLGEYGLSEEQGNEIIMAARAHWFDDEESSEPATAPAAEPQNGEAADADPAQ
>Mature_547_residues
ATAISANKAELLAIANSVASEKMIDKAIVIEAIEEAIQRAARARYGAENDIRAKLDPQTGDLRLWRVVEVVETVEDYFKQ
VDLAAGQKLQKDARIGDFIVDPLPAVDLGRIDAQSAKQVIFQKVREADRERQYQEFKDRAGEIITGVVKSVEFGHIVVNL
GRAEGVIRRDQQIPRELMRVGDRVRALILSVRSETRGPQIFLSRAHPDFMKKLFAQEVPEIYDGIIEIKAAARDPGSRAK
IGVISYDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVQRVVIDEDDGRIEVVVPDDQLS
LAIGRRGQNVRLASQLTGKQIDIMTEADASEKRQREFVERSTMFQEELDVDETLAQLLVAEGFSELEEVAYVPLDELASI
EGFDEELAQELQSRAAEGLERREEAARAERRELGVEDALADIPHLTEAMLVTLGKAGIRTLDDLADLATDELIAKKRTDN
RRGPARSERAEDKGGVLGEYGLSEEQGNEIIMAARAHWFDDEESSEPATAPAAEPQNGEAADADPAQ

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=518, Percent_Identity=43.050193050193, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 60411; Mature: 60279

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAISANKAELLAIANSVASEKMIDKAIVIEAIEEAIQRAARARYGAENDIRAKLDPQT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
GDLRLWRVVEVVETVEDYFKQVDLAAGQKLQKDARIGDFIVDPLPAVDLGRIDAQSAKQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHH
IFQKVREADRERQYQEFKDRAGEIITGVVKSVEFGHIVVNLGRAEGVIRRDQQIPRELMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHCCHHHHHHHHHHHHHH
VGDRVRALILSVRSETRGPQIFLSRAHPDFMKKLFAQEVPEIYDGIIEIKAAARDPGSRA
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCCC
KIGVISYDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVQ
EEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCEE
RVVIDEDDGRIEVVVPDDQLSLAIGRRGQNVRLASQLTGKQIDIMTEADASEKRQREFVE
EEEEECCCCEEEEEECCCCEEHHCCCCCCCEEHHHHHCCCEEEEEECCCHHHHHHHHHHH
RSTMFQEELDVDETLAQLLVAEGFSELEEVAYVPLDELASIEGFDEELAQELQSRAAEGL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
ERREEAARAERRELGVEDALADIPHLTEAMLVTLGKAGIRTLDDLADLATDELIAKKRTD
HHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRRGPARSERAEDKGGVLGEYGLSEEQGNEIIMAARAHWFDDEESSEPATAPAAEPQNGE
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCC
AADADPAQ
CCCCCCCC
>Mature Secondary Structure 
ATAISANKAELLAIANSVASEKMIDKAIVIEAIEEAIQRAARARYGAENDIRAKLDPQT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
GDLRLWRVVEVVETVEDYFKQVDLAAGQKLQKDARIGDFIVDPLPAVDLGRIDAQSAKQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHH
IFQKVREADRERQYQEFKDRAGEIITGVVKSVEFGHIVVNLGRAEGVIRRDQQIPRELMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHCCHHHHHHHHHHHHHH
VGDRVRALILSVRSETRGPQIFLSRAHPDFMKKLFAQEVPEIYDGIIEIKAAARDPGSRA
HHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCCC
KIGVISYDSSIDPVGACVGMKGSRVQAVVQELQGEKIDIIPWSEDTATFVVNALQPATVQ
EEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCEE
RVVIDEDDGRIEVVVPDDQLSLAIGRRGQNVRLASQLTGKQIDIMTEADASEKRQREFVE
EEEEECCCCEEEEEECCCCEEHHCCCCCCCEEHHHHHCCCEEEEEECCCHHHHHHHHHHH
RSTMFQEELDVDETLAQLLVAEGFSELEEVAYVPLDELASIEGFDEELAQELQSRAAEGL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
ERREEAARAERRELGVEDALADIPHLTEAMLVTLGKAGIRTLDDLADLATDELIAKKRTD
HHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRRGPARSERAEDKGGVLGEYGLSEEQGNEIIMAARAHWFDDEESSEPATAPAAEPQNGE
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCC
AADADPAQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]