Click here to switch to the map view.

The map label for this gene is purA

Identifier: 103486003

GI number: 103486003

Start: 527847

End: 529136

Strand: Reverse

Name: purA

Synonym: Sala_0510

Alternate gene names: 103486003

Gene position: 529136-527847 (Counterclockwise)

Preceding gene: 103486005

Following gene: 103486002

Centisome position: 15.82

GC content: 66.98

Gene sequence:

>1290_bases
ATGGCAAATGTTACCGTCATCGGGTCGCAATGGGGCGACGAGGGCAAGGGCAAGATCGTCGACTGGCTCGCCAGCCGTGC
CGATGCCGTGGTCCGGTTCCAGGGCGGTCACAACGCCGGCCATACGCTCGTCGTCGGCGAGCAGGTGTACAAGCTGTCGC
TGCTGCCTTCGGGCATCGTCACCGGCACGCTGTCGATCATCGGCAATGGCGTCGTGCTCGATCCCTGGGCGCTGCGCGAC
GAGATCACCAAGCTGCGCGGCCAGGGGGTCGAGATCAACGCCGACAATTTCGCGATCGCCGACAATTGCGCGCTGATCCT
GCCCTTTCACCGCGACCTCGACGCGCTGCGCGAAACCGCGGCGGGCGCGGGCAAGATCGGCACCACCGGGCGCGGCATCG
GCCCGGCCTATGAGGACAAGGTCGGTCGCCGCGCGATCCGCGTCTGCGACCTGGCGCACCTCGACCATCTCGAACCCCAG
CTCGACCGGCTGACCGCGCACCACGACGCGCTGCGCGCGGGCTTCGGCGAACCGCCCATCGACCGCGACAAGCTGGTCGC
CGACCTCAGGGAAATCGCCGATTATGTGCTCGAATATGCGCAGCCGGTGTGGAAGCGGCTGAAAAAGGTCCGCAAGGCCG
GCGCGCGCATATTGTTCGAGGGCGCGCAGGGCGTGCTGCTCGACATCGACCACGGCACCTATCCCTTTGTCACCAGCTCG
AACACGGTGAGCGGCACCGCGGCCTCGGGATCGGGTCTCGGCCCCGGCGCGGTCGGCTTCGTGCTCGGCATCGCCAAGGC
CTATACCACCCGCGTCGGCAGCGGCCCCTTCCCCACCGAACTGGAGGATGAAACGGGCCAGCGTTTGGGTGAACGCGGGC
ATGAGTTCGGGACCGTCACCGGGCGCAAGCGCCGCTGCGGCTGGTTCGACGCCGTGCTCGTGCGGCAGAGCTGCGCGGTA
TCGGGCGTCACCGGCATCGCGCTGACCAAGCTCGACGTGCTCGACGGCTTTGACACGATCCGCATCTGCACCGGCTACCG
TCTCAGGGGAAAAATCCTCGACTATTTCCCCGCGCACGCCGCCGATCAGGCCGCGGTCGAGCCGATTTACGAGGAAATGG
ACGGCTGGCACGAATCGACCGCGGGCGCACGCAGCTATGCCGACCTGCCCGCGCAGGCGATCAAATATATCCAGCGCGTG
CAGGAACTGATCGAAACGCCGATCGCGCTGGTGTCGACCAGCCCCGAACGCGAGGACACGATCCTGATCCGCGATCCCTT
CAGCGACTAG

Upstream 100 bases:

>100_bases
CCGAAATTGTCGCAATTTCGTGCCAGCCGCGCTTGTATCCGCGCGCAAATCCGCTAAGGCCGCGCCGGGCCGAAAGGGCC
GCTTTCAGCAGGACAGCGTC

Downstream 100 bases:

>100_bases
GACGCCACGCCCTTCCTTTCCGGCACCGGCCGTGGCACCAAGGCGCCATGACCCGCACCATCCTTGTCCTTTATGGCAGC
TATCGCCGCGACCGGCAGGG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MANVTVIGSQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLVVGEQVYKLSLLPSGIVTGTLSIIGNGVVLDPWALRD
EITKLRGQGVEINADNFAIADNCALILPFHRDLDALRETAAGAGKIGTTGRGIGPAYEDKVGRRAIRVCDLAHLDHLEPQ
LDRLTAHHDALRAGFGEPPIDRDKLVADLREIADYVLEYAQPVWKRLKKVRKAGARILFEGAQGVLLDIDHGTYPFVTSS
NTVSGTAASGSGLGPGAVGFVLGIAKAYTTRVGSGPFPTELEDETGQRLGERGHEFGTVTGRKRRCGWFDAVLVRQSCAV
SGVTGIALTKLDVLDGFDTIRICTGYRLRGKILDYFPAHAADQAAVEPIYEEMDGWHESTAGARSYADLPAQAIKYIQRV
QELIETPIALVSTSPEREDTILIRDPFSD

Sequences:

>Translated_429_residues
MANVTVIGSQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLVVGEQVYKLSLLPSGIVTGTLSIIGNGVVLDPWALRD
EITKLRGQGVEINADNFAIADNCALILPFHRDLDALRETAAGAGKIGTTGRGIGPAYEDKVGRRAIRVCDLAHLDHLEPQ
LDRLTAHHDALRAGFGEPPIDRDKLVADLREIADYVLEYAQPVWKRLKKVRKAGARILFEGAQGVLLDIDHGTYPFVTSS
NTVSGTAASGSGLGPGAVGFVLGIAKAYTTRVGSGPFPTELEDETGQRLGERGHEFGTVTGRKRRCGWFDAVLVRQSCAV
SGVTGIALTKLDVLDGFDTIRICTGYRLRGKILDYFPAHAADQAAVEPIYEEMDGWHESTAGARSYADLPAQAIKYIQRV
QELIETPIALVSTSPEREDTILIRDPFSD
>Mature_428_residues
ANVTVIGSQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLVVGEQVYKLSLLPSGIVTGTLSIIGNGVVLDPWALRDE
ITKLRGQGVEINADNFAIADNCALILPFHRDLDALRETAAGAGKIGTTGRGIGPAYEDKVGRRAIRVCDLAHLDHLEPQL
DRLTAHHDALRAGFGEPPIDRDKLVADLREIADYVLEYAQPVWKRLKKVRKAGARILFEGAQGVLLDIDHGTYPFVTSSN
TVSGTAASGSGLGPGAVGFVLGIAKAYTTRVGSGPFPTELEDETGQRLGERGHEFGTVTGRKRRCGWFDAVLVRQSCAVS
GVTGIALTKLDVLDGFDTIRICTGYRLRGKILDYFPAHAADQAAVEPIYEEMDGWHESTAGARSYADLPAQAIKYIQRVQ
ELIETPIALVSTSPEREDTILIRDPFSD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=43.1279620853081, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI34577063, Length=429, Percent_Identity=40.5594405594406, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=41.1616161616162, Blast_Score=280, Evalue=1e-75,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=50.9345794392523, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=42.3887587822014, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=42.3887587822014, Blast_Score=332, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=39.3518518518519, Blast_Score=301, Evalue=1e-82,
Organism=Drosophila melanogaster, GI21356033, Length=419, Percent_Identity=41.0501193317422, Blast_Score=313, Evalue=1e-85,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_SPHAL (Q1GVU1)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615564.1
- ProteinModelPortal:   Q1GVU1
- SMR:   Q1GVU1
- GeneID:   4081400
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0510
- NMPDR:   fig|317655.9.peg.468
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   SALA317655:SALA_0510-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46275; Mature: 46144

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVTVIGSQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLVVGEQVYKLSLLPSGIV
CCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEECCCEEEEECCCCCHH
TGTLSIIGNGVVLDPWALRDEITKLRGQGVEINADNFAIADNCALILPFHRDLDALRETA
HHHHHHHCCCEEECCHHHHHHHHHHCCCCEEECCCCEEEECCCEEEEECHHHHHHHHHHH
AGAGKIGTTGRGIGPAYEDKVGRRAIRVCDLAHLDHLEPQLDRLTAHHDALRAGFGEPPI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
DRDKLVADLREIADYVLEYAQPVWKRLKKVRKAGARILFEGAQGVLLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCEEECC
NTVSGTAASGSGLGPGAVGFVLGIAKAYTTRVGSGPFPTELEDETGQRLGERGHEFGTVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
GRKRRCGWFDAVLVRQSCAVSGVTGIALTKLDVLDGFDTIRICTGYRLRGKILDYFPAHA
CCCCCCCHHHHHHHHHHHHHCCCCHHHEEHHHHHCCCCEEEEECCCEEECHHHHHCCCCC
ADQAAVEPIYEEMDGWHESTAGARSYADLPAQAIKYIQRVQELIETPIALVSTSPEREDT
CCHHHHHHHHHHHCCHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCE
ILIRDPFSD
EEEECCCCC
>Mature Secondary Structure 
ANVTVIGSQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLVVGEQVYKLSLLPSGIV
CCEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEECCCEEEEECCCCCHH
TGTLSIIGNGVVLDPWALRDEITKLRGQGVEINADNFAIADNCALILPFHRDLDALRETA
HHHHHHHCCCEEECCHHHHHHHHHHCCCCEEECCCCEEEECCCEEEEECHHHHHHHHHHH
AGAGKIGTTGRGIGPAYEDKVGRRAIRVCDLAHLDHLEPQLDRLTAHHDALRAGFGEPPI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
DRDKLVADLREIADYVLEYAQPVWKRLKKVRKAGARILFEGAQGVLLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCEEECC
NTVSGTAASGSGLGPGAVGFVLGIAKAYTTRVGSGPFPTELEDETGQRLGERGHEFGTVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
GRKRRCGWFDAVLVRQSCAVSGVTGIALTKLDVLDGFDTIRICTGYRLRGKILDYFPAHA
CCCCCCCHHHHHHHHHHHHHCCCCHHHEEHHHHHCCCCEEEEECCCEEECHHHHHCCCCC
ADQAAVEPIYEEMDGWHESTAGARSYADLPAQAIKYIQRVQELIETPIALVSTSPEREDT
CCHHHHHHHHHHHCCHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCE
ILIRDPFSD
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA