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The map label for this gene is outK [H]

Identifier: 103485817

GI number: 103485817

Start: 328966

End: 329970

Strand: Direct

Name: outK [H]

Synonym: Sala_0322

Alternate gene names: 103485817

Gene position: 328966-329970 (Clockwise)

Preceding gene: 103485816

Following gene: 103485818

Centisome position: 9.83

GC content: 70.15

Gene sequence:

>1005_bases
ATGACCCCGGCACCGGGCGATCGCGGCGCGGCTTTGCTGAGCGTCCTGCTGCTCGTCGCGGTGATGGCGGTGATCGCGGC
GACCGCGCTCGACCGGCTGACGCTCGCGACGCGGATCGCGGGCAGCGCGGCGACGGTCGATCAGGGACGCGCCTACAGCT
TTGCCGCCGAACAGATTGCGCTTGGCAGGGTCGCCGATCTGGTCGGGCGCGATCCGGCGAAGCTGACGCTGGCGGGCGAC
TGGCTGGGGCGCGATTTCGTGCTGCCATTGCCCGGTGGCGAGGGACGCGCGCGGCTGACCGACGCCAGCAATTGCTTCAA
CCTCAACAGCCTGGTCGCCGAAACGCTGCCGGGGCGCTTGACGCAGCGCATGGGTGCGCAGCGGCAATTCGCCGCGTTGA
TGACCCTGCTCGGCATCGAGGATGGCGAGGCGCAGGCAATCGCGGGCGCCGCGGCCGACTGGATCGACAGCGACAGCAAC
GAAGGACGGCTCGGCGCCGAGGATAATGTCTACCGGTCGCTGCCCGGCGCCTATCTGCCTGCGAACCGCAAGATGGCGCA
TGTCAGCGAACTGCGCGCGGTGCGCGGCGTCAGCGCCAAACTCTACGCGCAGCTGGAACCCTGGGTCTGCGTGCTGCCCA
CGACCGACCCGGTGAAGCTCAATGTCAACACGCTCGCCGCCTGGCAGGCGCCGCTCGTCGCGATGCTGTGGCCCGGCGAA
ATCGACGTCAGGGCCGCACGGGCGGCGCTAGCCGCGCGGCCCGCGGGCGGCTATGGCAGCAGCGTGCGATTCTGGGAGGC
GTCGGCGTTCGATCGCCGCAAGCCGCCGGACGATGTCGCCGAACAGGCGGCGGTGACGAGCCGCTGGCTGGTGCTGACGA
CGAATGTGACGATGGGGGACGGGTTTCTGACGGCAGTTTCACTCATCGACGCCAATGGCGGCGCGCCGTCGGTCGGCACG
GCCCCTCCGGTGATCGTGCGCCGCGACTGGGGCGAAAGCGACTGA

Upstream 100 bases:

>100_bases
TCGAGGTGCGGATCGACCGAACGGGCCGCCCGCCGCTGACGATGCTGTTCCTCACCGCGCCGACGCCATTGCCGCCGCCC
GCCATCGCGGGAGAGGCGCC

Downstream 100 bases:

>100_bases
CGTGACGCGCACCTTGCTGATCTGGTTGCCGCGAGTGGCGGCGCTTGCGGACGCCGCCGCCGCGCCCGCGGCGTGGCTTC
GCATCGACGACGGCGTTGTC

Product: general secretion pathway protein K

Products: NA

Alternate protein names: Pectic enzymes secretion protein OUTK [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTPAPGDRGAALLSVLLLVAVMAVIAATALDRLTLATRIAGSAATVDQGRAYSFAAEQIALGRVADLVGRDPAKLTLAGD
WLGRDFVLPLPGGEGRARLTDASNCFNLNSLVAETLPGRLTQRMGAQRQFAALMTLLGIEDGEAQAIAGAAADWIDSDSN
EGRLGAEDNVYRSLPGAYLPANRKMAHVSELRAVRGVSAKLYAQLEPWVCVLPTTDPVKLNVNTLAAWQAPLVAMLWPGE
IDVRAARAALAARPAGGYGSSVRFWEASAFDRRKPPDDVAEQAAVTSRWLVLTTNVTMGDGFLTAVSLIDANGGAPSVGT
APPVIVRRDWGESD

Sequences:

>Translated_334_residues
MTPAPGDRGAALLSVLLLVAVMAVIAATALDRLTLATRIAGSAATVDQGRAYSFAAEQIALGRVADLVGRDPAKLTLAGD
WLGRDFVLPLPGGEGRARLTDASNCFNLNSLVAETLPGRLTQRMGAQRQFAALMTLLGIEDGEAQAIAGAAADWIDSDSN
EGRLGAEDNVYRSLPGAYLPANRKMAHVSELRAVRGVSAKLYAQLEPWVCVLPTTDPVKLNVNTLAAWQAPLVAMLWPGE
IDVRAARAALAARPAGGYGSSVRFWEASAFDRRKPPDDVAEQAAVTSRWLVLTTNVTMGDGFLTAVSLIDANGGAPSVGT
APPVIVRRDWGESD
>Mature_333_residues
TPAPGDRGAALLSVLLLVAVMAVIAATALDRLTLATRIAGSAATVDQGRAYSFAAEQIALGRVADLVGRDPAKLTLAGDW
LGRDFVLPLPGGEGRARLTDASNCFNLNSLVAETLPGRLTQRMGAQRQFAALMTLLGIEDGEAQAIAGAAADWIDSDSNE
GRLGAEDNVYRSLPGAYLPANRKMAHVSELRAVRGVSAKLYAQLEPWVCVLPTTDPVKLNVNTLAAWQAPLVAMLWPGEI
DVRAARAALAARPAGGYGSSVRFWEASAFDRRKPPDDVAEQAAVTSRWLVLTTNVTMGDGFLTAVSLIDANGGAPSVGTA
PPVIVRRDWGESD

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=242, Percent_Identity=31.404958677686, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 35110; Mature: 34979

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAPGDRGAALLSVLLLVAVMAVIAATALDRLTLATRIAGSAATVDQGRAYSFAAEQIA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LGRVADLVGRDPAKLTLAGDWLGRDFVLPLPGGEGRARLTDASNCFNLNSLVAETLPGRL
HHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHCCHHH
TQRMGAQRQFAALMTLLGIEDGEAQAIAGAAADWIDSDSNEGRLGAEDNVYRSLPGAYLP
HHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCC
ANRKMAHVSELRAVRGVSAKLYAQLEPWVCVLPTTDPVKLNVNTLAAWQAPLVAMLWPGE
CCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCEEEEHHHHHHHCCCEEEEECCCC
IDVRAARAALAARPAGGYGSSVRFWEASAFDRRKPPDDVAEQAAVTSRWLVLTTNVTMGD
CCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEEEEEECEEECC
GFLTAVSLIDANGGAPSVGTAPPVIVRRDWGESD
CCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TPAPGDRGAALLSVLLLVAVMAVIAATALDRLTLATRIAGSAATVDQGRAYSFAAEQIA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LGRVADLVGRDPAKLTLAGDWLGRDFVLPLPGGEGRARLTDASNCFNLNSLVAETLPGRL
HHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHCCHHH
TQRMGAQRQFAALMTLLGIEDGEAQAIAGAAADWIDSDSNEGRLGAEDNVYRSLPGAYLP
HHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCC
ANRKMAHVSELRAVRGVSAKLYAQLEPWVCVLPTTDPVKLNVNTLAAWQAPLVAMLWPGE
CCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCEEEEHHHHHHHCCCEEEEECCCC
IDVRAARAALAARPAGGYGSSVRFWEASAFDRRKPPDDVAEQAAVTSRWLVLTTNVTMGD
CCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEEEEEECEEECC
GFLTAVSLIDANGGAPSVGTAPPVIVRRDWGESD
CCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]