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The map label for this gene is ispG [H]

Identifier: 159185347

GI number: 159185347

Start: 2708217

End: 2709467

Strand: Reverse

Name: ispG [H]

Synonym: Atu2723

Alternate gene names: 159185347

Gene position: 2709467-2708217 (Counterclockwise)

Preceding gene: 159185348

Following gene: 15889974

Centisome position: 95.35

GC content: 59.47

Gene sequence:

>1251_bases
ATGAATTCACCTGCCGATTACGACCCGAAGCCCCGCCGCGCATCCGTCGCTGTCGACGTAGGCGGCGTTATCGTGGGAGG
CGGTGCACCCGTCGTGGTGCAGTCGATGACCAATACCGATACGGCTGACATCGACGCCACCGTCCGCCAAGTGGCGGCAC
TTTTCCAGGCCGGTTCGGAAATGGTGCGCATTACCGTCGACCGAGACGAAAGTGCTGCCGCCGTGCCGAAAATCCGCGAG
CGGCTGCTACGACTTGGCATGGATGTGCCGCTGATCGGTGATTTTCACTATATCGGCCATAAGCTTCTGGCCGATCATCC
GGCCTGTGCGGAAGCGCTGGCCAAATACCGCATCAATCCCGGCAATGTCGGTTTCAAGGACAAGAAGGACAAGCAGTTCG
GCGAGATCGTCGAAATGGCGATCCGTTATGACAAGCCCGTCCGCATCGGCGTCAACTGGGGGTCGCTCGATCAGGAACTG
CTGACGACGCTGATGGACAGAAACAAGGAGCAGGGTTTTCCGCTCTCCGCCCGTCAGGTTACGCGGGAAGCCATCGTTCA
GTCGGCACTGATTTCCGCCGAGCTTGCCGAAGAGATCGGCCTGCCGCGCAACCGCATCATCCTGTCGGCCAAGGTCAGTC
AGGTGCAGGACCTGATCGCCGTTTATTCGATGCTGTCGGAACGATCCAATCACGCCCTGCATTTGGGCTTGACCGAGGCG
GGCATGGGTTCGAAGGGCATCGTCGCTTCGTCAGCTGCCATGGGTTACGTGCTGCAGCATGGCATTGGCGATACGATCCG
CGTTTCGCTGACGCCTGAGCCGAATGGCGACCGCACCCGTGAGGTGCAGGTGGCGCAGGAACTGCTGCAGGTCATGGGCT
TTCGGCAGTTCATCCCGGTCGTTGCGGCATGTCCCGGCTGCGGGCGCACCACGTCCACGGTATTTCAGGAACTGGCGCAG
AACATCCAGAACGACATCCGCAAGAACATGCCGATCTGGCGTGAAAAATATCCAGGGGTCGAGGCGCTCAACGTCGCCGT
CATGGGCTGCATCGTCAACGGCCCCGGTGAAAGCAAACATGCCGATATCGGCATTTCACTGCCCGGAACCGGCGAAAGCC
CGGCTGCACCTGTTTTCATCGATGGCGAAAAGGCTCTCACGCTACGTGGTCCCAATATCGCCGCCGATTTTGAAGCGCTT
GTGATCGATTACATCGAAAAGCGTTTTGGCCAGAAAGATGCGGCGGAGTAA

Upstream 100 bases:

>100_bases
ATTGCGGTAAAGTAGAAGCTCCAGCGCGTTGGGTGTTGACAAGCAAGCCCCCTTGTTTCACCTCAATTGCAAGACCTTGC
CGCAAGCTCCAGAGACCGCC

Downstream 100 bases:

>100_bases
TGCGTAAGTTGCAACGGCCTGAATTTCACTGTCATACCTCCTGAACCTCTCGGAGCATCCAGAAAAATGATCAAAAAAAT
CGCGATCGTCGCCCTTTGCG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNSPADYDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVRQVAALFQAGSEMVRITVDRDESAAAVPKIRE
RLLRLGMDVPLIGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFGEIVEMAIRYDKPVRIGVNWGSLDQEL
LTTLMDRNKEQGFPLSARQVTREAIVQSALISAELAEEIGLPRNRIILSAKVSQVQDLIAVYSMLSERSNHALHLGLTEA
GMGSKGIVASSAAMGYVLQHGIGDTIRVSLTPEPNGDRTREVQVAQELLQVMGFRQFIPVVAACPGCGRTTSTVFQELAQ
NIQNDIRKNMPIWREKYPGVEALNVAVMGCIVNGPGESKHADIGISLPGTGESPAAPVFIDGEKALTLRGPNIAADFEAL
VIDYIEKRFGQKDAAE

Sequences:

>Translated_416_residues
MNSPADYDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVRQVAALFQAGSEMVRITVDRDESAAAVPKIRE
RLLRLGMDVPLIGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFGEIVEMAIRYDKPVRIGVNWGSLDQEL
LTTLMDRNKEQGFPLSARQVTREAIVQSALISAELAEEIGLPRNRIILSAKVSQVQDLIAVYSMLSERSNHALHLGLTEA
GMGSKGIVASSAAMGYVLQHGIGDTIRVSLTPEPNGDRTREVQVAQELLQVMGFRQFIPVVAACPGCGRTTSTVFQELAQ
NIQNDIRKNMPIWREKYPGVEALNVAVMGCIVNGPGESKHADIGISLPGTGESPAAPVFIDGEKALTLRGPNIAADFEAL
VIDYIEKRFGQKDAAE
>Mature_416_residues
MNSPADYDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVRQVAALFQAGSEMVRITVDRDESAAAVPKIRE
RLLRLGMDVPLIGDFHYIGHKLLADHPACAEALAKYRINPGNVGFKDKKDKQFGEIVEMAIRYDKPVRIGVNWGSLDQEL
LTTLMDRNKEQGFPLSARQVTREAIVQSALISAELAEEIGLPRNRIILSAKVSQVQDLIAVYSMLSERSNHALHLGLTEA
GMGSKGIVASSAAMGYVLQHGIGDTIRVSLTPEPNGDRTREVQVAQELLQVMGFRQFIPVVAACPGCGRTTSTVFQELAQ
NIQNDIRKNMPIWREKYPGVEALNVAVMGCIVNGPGESKHADIGISLPGTGESPAAPVFIDGEKALTLRGPNIAADFEAL
VIDYIEKRFGQKDAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=399, Percent_Identity=31.328320802005, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44874; Mature: 44874

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPADYDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVRQVAALFQAGSE
CCCCCCCCCCCCCEEEEEEECCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
MVRITVDRDESAAAVPKIRERLLRLGMDVPLIGDFHYIGHKLLADHPACAEALAKYRINP
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHCCCHHHHHHHHHHCCCC
GNVGFKDKKDKQFGEIVEMAIRYDKPVRIGVNWGSLDQELLTTLMDRNKEQGFPLSARQV
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
TREAIVQSALISAELAEEIGLPRNRIILSAKVSQVQDLIAVYSMLSERSNHALHLGLTEA
HHHHHHHHHHHHHHHHHHHCCCHHEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHC
GMGSKGIVASSAAMGYVLQHGIGDTIRVSLTPEPNGDRTREVQVAQELLQVMGFRQFIPV
CCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAACPGCGRTTSTVFQELAQNIQNDIRKNMPIWREKYPGVEALNVAVMGCIVNGPGESKH
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHEEEEEEEECCCCCCCC
ADIGISLPGTGESPAAPVFIDGEKALTLRGPNIAADFEALVIDYIEKRFGQKDAAE
CEEEEECCCCCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNSPADYDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVRQVAALFQAGSE
CCCCCCCCCCCCCEEEEEEECCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
MVRITVDRDESAAAVPKIRERLLRLGMDVPLIGDFHYIGHKLLADHPACAEALAKYRINP
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHCCCHHHHHHHHHHCCCC
GNVGFKDKKDKQFGEIVEMAIRYDKPVRIGVNWGSLDQELLTTLMDRNKEQGFPLSARQV
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
TREAIVQSALISAELAEEIGLPRNRIILSAKVSQVQDLIAVYSMLSERSNHALHLGLTEA
HHHHHHHHHHHHHHHHHHHCCCHHEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHC
GMGSKGIVASSAAMGYVLQHGIGDTIRVSLTPEPNGDRTREVQVAQELLQVMGFRQFIPV
CCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAACPGCGRTTSTVFQELAQNIQNDIRKNMPIWREKYPGVEALNVAVMGCIVNGPGESKH
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHEEEEEEEECCCCCCCC
ADIGISLPGTGESPAAPVFIDGEKALTLRGPNIAADFEALVIDYIEKRFGQKDAAE
CEEEEECCCCCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA