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The map label for this gene is miaA

Identifier: 159185023

GI number: 159185023

Start: 1998197

End: 1999093

Strand: Reverse

Name: miaA

Synonym: Atu2039

Alternate gene names: 159185023

Gene position: 1999093-1998197 (Counterclockwise)

Preceding gene: 15889329

Following gene: 159185021

Centisome position: 70.35

GC content: 62.54

Gene sequence:

>897_bases
ATGATGAAAAACCTTGATCAGAATTTTGATGCGATCCTGATAACCGGCCCGACGGCAAGCGGCAAGTCCGCGCTTGCGCT
TCGTCTGGCGCGGGAGCGGAACGGCGTCGTCATCAATGCCGACAGCATGCAGGTTTACGACACGCTGCGGGTGCTGACCG
CCCGGCCTTCCGACCACGAAATGGAGGGGGTGCCACACCGTCTCTACGGCCATGTGCCCGCTGGCAGCGCCTATTCGACC
GGCGAATGGCTGCGGGATATTTCCGGACTGCTTTCGGATCTGCGCGGTGAGGGGCGTTTTCCTGTCATTGTCGGCGGTAC
GGGGCTTTATTTCAAGGCGCTGACCGGCGGCCTTTCCGATATGCCCGCCATTCCCGATGACCTCCGCGAGGGGCTGCGCG
CCCGGTTGATCGAAGAGGGAGCGGCAAAGCTTCACGCCGAATTGGTGAGCCGCGATCCGTCCATGGCGCAGATGCTGCAG
CCGGGAGATGGCCAGCGCATCGTCCGGGCACTGGAGGTGCTCGAGGCGACGGGGAAATCGATCCGCGATTTCCAGCGCGC
CAGCGGCCCGATGATCATCGATCCCGAGCGGGCGCAGAAATTCATCGTCCTGCCGGAGAGGCCGGTGCTGCATGACCGTA
TCAACCGCCGTTTTGAGGCGATGATGGACAGTGGTGCGGTGGAGGAGGTCCAGGCGCTTCTTGCGCTCAATCTAGCGCCC
GATGCAACGGCGATGAAGGCGATTGGCGTTGCTCAGATCGCCGATATGCTGACTGGGCGCATGGGTGCGGCGGAGGTGAT
AGAAAAATCGGCGGCTGCGACCCGCCAATATGCCAAACGGCAGATGACCTGGTTCCGCAACCAGATGGGGGATGACTGGA
CGCGCATCCAGCCGTGA

Upstream 100 bases:

>100_bases
ATAAAGGCCCGATGCATTGACCGCCTTTGCGGCGGCTTCGCCGAGGGCTGCTGTCAGAACAGGATTTGACGGATTGGCGA
TAAGCGTGGCAACCAGAGCC

Downstream 100 bases:

>100_bases
AAGGGGCCTAATCCTTGCGGTAGAGGCTGCCGAGCGGTTTTTTCAACAGGCTTTCGCGCAACGAGGGGCGCGTCTCACCG
GGCGGCGGGCCATTCTGCGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MMKNLDQNFDAILITGPTASGKSALALRLARERNGVVINADSMQVYDTLRVLTARPSDHEMEGVPHRLYGHVPAGSAYST
GEWLRDISGLLSDLRGEGRFPVIVGGTGLYFKALTGGLSDMPAIPDDLREGLRARLIEEGAAKLHAELVSRDPSMAQMLQ
PGDGQRIVRALEVLEATGKSIRDFQRASGPMIIDPERAQKFIVLPERPVLHDRINRRFEAMMDSGAVEEVQALLALNLAP
DATAMKAIGVAQIADMLTGRMGAAEVIEKSAAATRQYAKRQMTWFRNQMGDDWTRIQP

Sequences:

>Translated_298_residues
MMKNLDQNFDAILITGPTASGKSALALRLARERNGVVINADSMQVYDTLRVLTARPSDHEMEGVPHRLYGHVPAGSAYST
GEWLRDISGLLSDLRGEGRFPVIVGGTGLYFKALTGGLSDMPAIPDDLREGLRARLIEEGAAKLHAELVSRDPSMAQMLQ
PGDGQRIVRALEVLEATGKSIRDFQRASGPMIIDPERAQKFIVLPERPVLHDRINRRFEAMMDSGAVEEVQALLALNLAP
DATAMKAIGVAQIADMLTGRMGAAEVIEKSAAATRQYAKRQMTWFRNQMGDDWTRIQP
>Mature_298_residues
MMKNLDQNFDAILITGPTASGKSALALRLARERNGVVINADSMQVYDTLRVLTARPSDHEMEGVPHRLYGHVPAGSAYST
GEWLRDISGLLSDLRGEGRFPVIVGGTGLYFKALTGGLSDMPAIPDDLREGLRARLIEEGAAKLHAELVSRDPSMAQMLQ
PGDGQRIVRALEVLEATGKSIRDFQRASGPMIIDPERAQKFIVLPERPVLHDRINRRFEAMMDSGAVEEVQALLALNLAP
DATAMKAIGVAQIADMLTGRMGAAEVIEKSAAATRQYAKRQMTWFRNQMGDDWTRIQP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=327, Percent_Identity=25.3822629969419, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1790613, Length=285, Percent_Identity=37.8947368421053, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=25.3333333333333, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=299, Percent_Identity=29.0969899665552, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24649830, Length=330, Percent_Identity=24.5454545454545, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_AGRT5 (P0A3Q5)

Other databases:

- EMBL:   AE007869
- PIR:   AI2826
- PIR:   G97604
- RefSeq:   NP_355007.2
- STRING:   P0A3Q5
- GeneID:   1134077
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2039
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- PhylomeDB:   P0A3Q5
- ProtClustDB:   PRK00091
- BioCyc:   ATUM176299-1:ATU2039-MONOMER
- GO:   GO:0009405
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 32661; Mature: 32661

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKNLDQNFDAILITGPTASGKSALALRLARERNGVVINADSMQVYDTLRVLTARPSDHE
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCC
MEGVPHRLYGHVPAGSAYSTGEWLRDISGLLSDLRGEGRFPVIVGGTGLYFKALTGGLSD
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCC
MPAIPDDLREGLRARLIEEGAAKLHAELVSRDPSMAQMLQPGDGQRIVRALEVLEATGKS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCHH
IRDFQRASGPMIIDPERAQKFIVLPERPVLHDRINRRFEAMMDSGAVEEVQALLALNLAP
HHHHHHCCCCEEECHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
DATAMKAIGVAQIADMLTGRMGAAEVIEKSAAATRQYAKRQMTWFRNQMGDDWTRIQP
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMKNLDQNFDAILITGPTASGKSALALRLARERNGVVINADSMQVYDTLRVLTARPSDHE
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCC
MEGVPHRLYGHVPAGSAYSTGEWLRDISGLLSDLRGEGRFPVIVGGTGLYFKALTGGLSD
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCC
MPAIPDDLREGLRARLIEEGAAKLHAELVSRDPSMAQMLQPGDGQRIVRALEVLEATGKS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCHH
IRDFQRASGPMIIDPERAQKFIVLPERPVLHDRINRRFEAMMDSGAVEEVQALLALNLAP
HHHHHHCCCCEEECHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
DATAMKAIGVAQIADMLTGRMGAAEVIEKSAAATRQYAKRQMTWFRNQMGDDWTRIQP
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194