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The map label for this gene is ilvI [H]

Identifier: 159185021

GI number: 159185021

Start: 1994007

End: 1995800

Strand: Reverse

Name: ilvI [H]

Synonym: Atu2036

Alternate gene names: 159185021

Gene position: 1995800-1994007 (Counterclockwise)

Preceding gene: 159185023

Following gene: 15889323

Centisome position: 70.24

GC content: 58.08

Gene sequence:

>1794_bases
ATGACGGATAAGGACAATACGGAAAACAGCAATCGCATGACAGGTGCGGAGATCGTTCTGCAGGCGCTGAAGGACAACGG
CGTCGAGCACATCTTCGGTTATCCCGGCGGTGCCGTTCTTCCGATTTATGACGAGATTTTCCAGCAGGAAGACATTCAGC
ACATCCTCGTGCGTCATGAGCAGGGTGCCGGCCACGCGGCCGAAGGGTATGCCCGTTCCACCGGCAAGGTCGGCGTCATG
CTCGTTACCTCCGGTCCCGGCGCCACCAATGCGGTGACGCCGCTGCAGGATGCGCTGATGGATTCCATTCCGCTCGTCTG
CCTGTCCGGCCAGGTTCCGACATCGCTGATCGGTTCCGACGCATTTCAGGAATGCGATACCGTCGGCATCACGCGCCCCT
GCACCAAGCACAACTGGCTGGTCAAGGACGTCAACGAACTGGCTGGCATCATCCATGAAGCCTTCCGCATCGCGCAGACT
GGCCGTCCGGGTCCCGTCGTTGTCGATATTCCGAAGGATATTCAGTTCGCCACCGGCACCTATACGCCGCCTTCCGCCGC
CATCCAGCAGAAGAGCTACAAGCCGAAGGTTCAGGGCGACCTCAACGCCATTCATGCTGCCATCGAACTGATGTCGAAAG
CGAAGAAGCCGGTTTTTTACACCGGCGGCGGCGTCATCAATTCTGGTCCGGAGGCAACCCGTCTGCTGCGTGAACTGGTC
GAGCTCACCAATTTCCCGATCACCTCGACGCTGATGGGTCTCGGCGCCTATCCGGCTTCCGGCAAGAACTGGCTCGGCAT
GCTGGGCATGCACGGTTCCTACGAAGCCAATATGACGATGCATGATTGCGATGTCATGGTCTGCATCGGCGCGCGTTTCG
ATGACCGTATTACCGGTCGCATCAATGCGTTTTCGCCGAATTCCAAGAAGATCCATATCGATATCGATCCGTCCTCGATC
AACAAGACCGTGCGTGTCGACGTGCCTGTTATCGGCGATGTCGGGCATGTTCTGGAAGACATGGTTCGCCTGTGGCGCGC
TTTGCCGAAGAAACCGGAAAAAGCGCAGACGGCCGACTGGTGGTCGCAGATTGAGCGCTGGCGCGCCCGCAATTCCTTCG
CCTACAAGAACTCCAAAGATGTCATCATGCCGCAATATGCATTGCAGCGGCTTTATGAGGCGTCGAAGGGACGCGACACT
TACATCACGACAGAAGTCGGCCAGCACCAGATGTGGGCGGCGCAGTTCTTCGGTTTCGAGGAGCCGAACCACTGGATGAC
CTCGGGCGGTCTCGGCACCATGGGTTATGGCCTGCCGGCGGCTATCGGCGTGCAGGTCGCGCATCCCGAAGCGCTTGTCA
TCGACATTGCCGGTGACGCATCGATCCAGATGTGTATCCAGGAAATGTCCTGCGCCATCCAGTACGGCCTGCCGGTGAAG
ATTTTCATCCTCAACAACCAGTATATGGGCATGGTGCGGCAATGGCAGCAGTTGCTGCACGGCAACCGTCTGTCCAACTC
CTATACCGAAGCCATGCCTGATTTCGTCAAGCTGGCGGAGGCCTATGGCGCTGTCGGCATGTATTGCGACGACCCGAAGG
AACTGGACGACAAGATCGCCGAGATGATTGCGGTCAACAAGCCGGTCATCTTCGATTGCCGCGTTGCCAATCTCGCCAAT
TGCTTCCCGATGATCCCATCTGGTAAAGCCCATAACGAGATGCTGTTGCCGGATGAGGCGACGGATGAAGCTGTTGCCAA
CGCCATCGATGCCAAGGGCCGTCAGCTAGTCTGA

Upstream 100 bases:

>100_bases
CATGCGCTGAACGAGGCTCCTCTAGGGGCCTTTTTTTATGTCTTCAAACAGGCCCTCGGGCTCCTCGATATCCAAAGCCA
AAGCGGGATGGAAACAGGCA

Downstream 100 bases:

>100_bases
GCCGGGAGAGGAAAAGGACATGAACGCACACCTACAACCCACCGGCTCCGCCTATTTCATCCAGAAGGAAACGGCTGCCG
TTGAAAATCACACGCTGTCG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MTDKDNTENSNRMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHEQGAGHAAEGYARSTGKVGVM
LVTSGPGATNAVTPLQDALMDSIPLVCLSGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVKDVNELAGIIHEAFRIAQT
GRPGPVVVDIPKDIQFATGTYTPPSAAIQQKSYKPKVQGDLNAIHAAIELMSKAKKPVFYTGGGVINSGPEATRLLRELV
ELTNFPITSTLMGLGAYPASGKNWLGMLGMHGSYEANMTMHDCDVMVCIGARFDDRITGRINAFSPNSKKIHIDIDPSSI
NKTVRVDVPVIGDVGHVLEDMVRLWRALPKKPEKAQTADWWSQIERWRARNSFAYKNSKDVIMPQYALQRLYEASKGRDT
YITTEVGQHQMWAAQFFGFEEPNHWMTSGGLGTMGYGLPAAIGVQVAHPEALVIDIAGDASIQMCIQEMSCAIQYGLPVK
IFILNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGMYCDDPKELDDKIAEMIAVNKPVIFDCRVANLAN
CFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRQLV

Sequences:

>Translated_597_residues
MTDKDNTENSNRMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHEQGAGHAAEGYARSTGKVGVM
LVTSGPGATNAVTPLQDALMDSIPLVCLSGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVKDVNELAGIIHEAFRIAQT
GRPGPVVVDIPKDIQFATGTYTPPSAAIQQKSYKPKVQGDLNAIHAAIELMSKAKKPVFYTGGGVINSGPEATRLLRELV
ELTNFPITSTLMGLGAYPASGKNWLGMLGMHGSYEANMTMHDCDVMVCIGARFDDRITGRINAFSPNSKKIHIDIDPSSI
NKTVRVDVPVIGDVGHVLEDMVRLWRALPKKPEKAQTADWWSQIERWRARNSFAYKNSKDVIMPQYALQRLYEASKGRDT
YITTEVGQHQMWAAQFFGFEEPNHWMTSGGLGTMGYGLPAAIGVQVAHPEALVIDIAGDASIQMCIQEMSCAIQYGLPVK
IFILNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGMYCDDPKELDDKIAEMIAVNKPVIFDCRVANLAN
CFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRQLV
>Mature_596_residues
TDKDNTENSNRMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHEQGAGHAAEGYARSTGKVGVML
VTSGPGATNAVTPLQDALMDSIPLVCLSGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVKDVNELAGIIHEAFRIAQTG
RPGPVVVDIPKDIQFATGTYTPPSAAIQQKSYKPKVQGDLNAIHAAIELMSKAKKPVFYTGGGVINSGPEATRLLRELVE
LTNFPITSTLMGLGAYPASGKNWLGMLGMHGSYEANMTMHDCDVMVCIGARFDDRITGRINAFSPNSKKIHIDIDPSSIN
KTVRVDVPVIGDVGHVLEDMVRLWRALPKKPEKAQTADWWSQIERWRARNSFAYKNSKDVIMPQYALQRLYEASKGRDTY
ITTEVGQHQMWAAQFFGFEEPNHWMTSGGLGTMGYGLPAAIGVQVAHPEALVIDIAGDASIQMCIQEMSCAIQYGLPVKI
FILNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGMYCDDPKELDDKIAEMIAVNKPVIFDCRVANLANC
FPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRQLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=580, Percent_Identity=26.551724137931, Blast_Score=169, Evalue=9e-42,
Organism=Homo sapiens, GI21361361, Length=567, Percent_Identity=23.9858906525573, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=50.7908611599297, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1790104, Length=580, Percent_Identity=40.8620689655172, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI1786717, Length=571, Percent_Identity=31.8739054290718, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=26.9439421338156, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=25.4480286738351, Blast_Score=145, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=543, Percent_Identity=27.4401473296501, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=543, Percent_Identity=27.4401473296501, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=537, Percent_Identity=24.2085661080074, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=45.360824742268, Blast_Score=512, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320816, Length=497, Percent_Identity=24.5472837022133, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19922626, Length=559, Percent_Identity=25.2236135957066, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65398; Mature: 65266

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKDNTENSNRMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHE
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCC
QGAGHAAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCLSGQVPTSLIGSD
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHCCH
AFQECDTVGITRPCTKHNWLVKDVNELAGIIHEAFRIAQTGRPGPVVVDIPKDIQFATGT
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCC
YTPPSAAIQQKSYKPKVQGDLNAIHAAIELMSKAKKPVFYTGGGVINSGPEATRLLRELV
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHH
ELTNFPITSTLMGLGAYPASGKNWLGMLGMHGSYEANMTMHDCDVMVCIGARFDDRITGR
HHHCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEECCCEEEEECCCCCCCEECE
INAFSPNSKKIHIDIDPSSINKTVRVDVPVIGDVGHVLEDMVRLWRALPKKPEKAQTADW
EEEECCCCCEEEEEECHHHCCCEEEEECCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHH
WSQIERWRARNSFAYKNSKDVIMPQYALQRLYEASKGRDTYITTEVGQHQMWAAQFFGFE
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCC
EPNHWMTSGGLGTMGYGLPAAIGVQVAHPEALVIDIAGDASIQMCIQEMSCAIQYGLPVK
CCCCEEECCCCCCCCCCCCHHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHEECCCCEE
IFILNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGMYCDDPKELDDKIA
EEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHH
EMIAVNKPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRQLV
HHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDKDNTENSNRMTGAEIVLQALKDNGVEHIFGYPGGAVLPIYDEIFQQEDIQHILVRHE
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCC
QGAGHAAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCLSGQVPTSLIGSD
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHCCH
AFQECDTVGITRPCTKHNWLVKDVNELAGIIHEAFRIAQTGRPGPVVVDIPKDIQFATGT
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCC
YTPPSAAIQQKSYKPKVQGDLNAIHAAIELMSKAKKPVFYTGGGVINSGPEATRLLRELV
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHH
ELTNFPITSTLMGLGAYPASGKNWLGMLGMHGSYEANMTMHDCDVMVCIGARFDDRITGR
HHHCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEECCCEEEEECCCCCCCEECE
INAFSPNSKKIHIDIDPSSINKTVRVDVPVIGDVGHVLEDMVRLWRALPKKPEKAQTADW
EEEECCCCCEEEEEECHHHCCCEEEEECCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHH
WSQIERWRARNSFAYKNSKDVIMPQYALQRLYEASKGRDTYITTEVGQHQMWAAQFFGFE
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCC
EPNHWMTSGGLGTMGYGLPAAIGVQVAHPEALVIDIAGDASIQMCIQEMSCAIQYGLPVK
CCCCEEECCCCCCCCCCCCHHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHEECCCCEE
IFILNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGMYCDDPKELDDKIA
EEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHH
EMIAVNKPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRQLV
HHHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]