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The map label for this gene is ycdT [H]

Identifier: 159184874

GI number: 159184874

Start: 1675687

End: 1676757

Strand: Direct

Name: ycdT [H]

Synonym: Atu1691

Alternate gene names: 159184874

Gene position: 1675687-1676757 (Clockwise)

Preceding gene: 15888995

Following gene: 15889001

Centisome position: 58.97

GC content: 58.26

Gene sequence:

>1071_bases
ATGAACACGGCATCCACGCCCAAAACCACGGGGCCCGACATTGCCGGGCAGATTGCCTATGCCATGCGGAGCATGGGTGT
TTCGCCGATCCCTCGTAATTATAGTCTGTTTTACGAAGCCTATATCGGCTCGAACCCGGCGCTCACGAAAGATCTGGCGG
CGCTTGGCAACCGGGTCACGCAGGAGGATCTGGACGAGTTGTCGTCGCGTTACGGCGAAGGCAATCCCAACCGCTCCATC
GACGACGCGCAGGACAAACTGCGGCGCGAGCTGGAGGCGCTGCTGGGCACGCTGAAGCGCGAACAATCCTCCATCGAGAA
TTACAACCGCATTCTCGGCGAAACGCGCCAGCGCATCGATGACAAGAACGCCGCCAGCAACAATATTCTGAGAAATGCGA
TTTCACTGCTGGCCGATGCCACCGGCTCGAAAATCATCGATGGCGAAAAGACGTTCAACGACGTCAACCGCCATGCCGAG
GAAATGCATCAGGTGCGCCTCGAGCTCGATGAATATAAGCGCATCGCCAACACCGATTCGCTGACGCGCCTCTCCAACCG
GCGCGCTTTCGACGACCGGCTGGCCTCCGTTTATGACAGCTCCATCGGCCTGCAATATACCACGCTCGTCCTTCTGGATA
TCGATCACTTCAAGCGAATCAACGATACGTTCGGCCATCCGGTCGGCGACAAGATTCTCGCCACCGTCGCCTCCGTCATC
CGCGCCAATGTGCGCAAGGATGGTTTCGTTGCCCGAAGCGGCGGCGAGGAATTCGCCATCATTCTCGATGGCAATACGCC
GGAAGAGGTGATGGTGATGTGCGAACGCATCCGCCTGTCGCTGGAAAGCACGCCCTTCCGCAATTCGAGATCAGGTGCCG
ATTACGGCACGGTCACCATCTCGATAGGCTTCGCATCAGCCGCACGCGCCAGCAATCCCAGCGAGCTTTACGGCCATGCC
GATACGGCGCTCTACCACGCCAAGGAAACCGGCCGGAACCGTTCCGTCTTTTACGAGGACGGCATGCAGAACAATTATAC
CGGCAAGAACTGGCTGATTTATCGGACGTGA

Upstream 100 bases:

>100_bases
GTTTCAGGGAACAAAATTTAGTTTTCCATAAATTAAGCTTTCCAAAACAGCTCGTATAATAATAATCATAGTCGATAAGC
AGACTCACTGGAGTTTCTTG

Downstream 100 bases:

>100_bases
AAATTAGAGGGCAGCAGCCGTTCCGTTGACGCCCTCATTCCTGTGATCGTCACAGGAATCCAGCCAGACCAAGTTCTTGG
GCGGGGTGACACAAGTGCAT

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MNTASTPKTTGPDIAGQIAYAMRSMGVSPIPRNYSLFYEAYIGSNPALTKDLAALGNRVTQEDLDELSSRYGEGNPNRSI
DDAQDKLRRELEALLGTLKREQSSIENYNRILGETRQRIDDKNAASNNILRNAISLLADATGSKIIDGEKTFNDVNRHAE
EMHQVRLELDEYKRIANTDSLTRLSNRRAFDDRLASVYDSSIGLQYTTLVLLDIDHFKRINDTFGHPVGDKILATVASVI
RANVRKDGFVARSGGEEFAIILDGNTPEEVMVMCERIRLSLESTPFRNSRSGADYGTVTISIGFASAARASNPSELYGHA
DTALYHAKETGRNRSVFYEDGMQNNYTGKNWLIYRT

Sequences:

>Translated_356_residues
MNTASTPKTTGPDIAGQIAYAMRSMGVSPIPRNYSLFYEAYIGSNPALTKDLAALGNRVTQEDLDELSSRYGEGNPNRSI
DDAQDKLRRELEALLGTLKREQSSIENYNRILGETRQRIDDKNAASNNILRNAISLLADATGSKIIDGEKTFNDVNRHAE
EMHQVRLELDEYKRIANTDSLTRLSNRRAFDDRLASVYDSSIGLQYTTLVLLDIDHFKRINDTFGHPVGDKILATVASVI
RANVRKDGFVARSGGEEFAIILDGNTPEEVMVMCERIRLSLESTPFRNSRSGADYGTVTISIGFASAARASNPSELYGHA
DTALYHAKETGRNRSVFYEDGMQNNYTGKNWLIYRT
>Mature_356_residues
MNTASTPKTTGPDIAGQIAYAMRSMGVSPIPRNYSLFYEAYIGSNPALTKDLAALGNRVTQEDLDELSSRYGEGNPNRSI
DDAQDKLRRELEALLGTLKREQSSIENYNRILGETRQRIDDKNAASNNILRNAISLLADATGSKIIDGEKTFNDVNRHAE
EMHQVRLELDEYKRIANTDSLTRLSNRRAFDDRLASVYDSSIGLQYTTLVLLDIDHFKRINDTFGHPVGDKILATVASVI
RANVRKDGFVARSGGEEFAIILDGNTPEEVMVMCERIRLSLESTPFRNSRSGADYGTVTISIGFASAARASNPSELYGHA
DTALYHAKETGRNRSVFYEDGMQNNYTGKNWLIYRT

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=42.8571428571429, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787802, Length=176, Percent_Identity=38.6363636363636, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=33.5195530726257, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=36.3095238095238, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787541, Length=185, Percent_Identity=34.0540540540541, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693134, Length=244, Percent_Identity=31.9672131147541, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=36.4161849710983, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=34.5454545454545, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=30.7262569832402, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788956, Length=183, Percent_Identity=32.2404371584699, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=33.1360946745562, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081974, Length=185, Percent_Identity=28.1081081081081, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=102, Percent_Identity=34.3137254901961, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39651; Mature: 39651

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTASTPKTTGPDIAGQIAYAMRSMGVSPIPRNYSLFYEAYIGSNPALTKDLAALGNRVT
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCC
QEDLDELSSRYGEGNPNRSIDDAQDKLRRELEALLGTLKREQSSIENYNRILGETRQRID
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKNAASNNILRNAISLLADATGSKIIDGEKTFNDVNRHAEEMHQVRLELDEYKRIANTDS
CCCCHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LTRLSNRRAFDDRLASVYDSSIGLQYTTLVLLDIDHFKRINDTFGHPVGDKILATVASVI
HHHHHHCHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHH
RANVRKDGFVARSGGEEFAIILDGNTPEEVMVMCERIRLSLESTPFRNSRSGADYGTVTI
HHHCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
SIGFASAARASNPSELYGHADTALYHAKETGRNRSVFYEDGMQNNYTGKNWLIYRT
EEECHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MNTASTPKTTGPDIAGQIAYAMRSMGVSPIPRNYSLFYEAYIGSNPALTKDLAALGNRVT
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCC
QEDLDELSSRYGEGNPNRSIDDAQDKLRRELEALLGTLKREQSSIENYNRILGETRQRID
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKNAASNNILRNAISLLADATGSKIIDGEKTFNDVNRHAEEMHQVRLELDEYKRIANTDS
CCCCHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LTRLSNRRAFDDRLASVYDSSIGLQYTTLVLLDIDHFKRINDTFGHPVGDKILATVASVI
HHHHHHCHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHH
RANVRKDGFVARSGGEEFAIILDGNTPEEVMVMCERIRLSLESTPFRNSRSGADYGTVTI
HHHCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
SIGFASAARASNPSELYGHADTALYHAKETGRNRSVFYEDGMQNNYTGKNWLIYRT
EEECHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]