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The map label for this gene is moaA

Identifier: 15889001

GI number: 15889001

Start: 1680160

End: 1681209

Strand: Direct

Name: moaA

Synonym: Atu1695

Alternate gene names: 15889001

Gene position: 1680160-1681209 (Clockwise)

Preceding gene: 159184874

Following gene: 159184875

Centisome position: 59.13

GC content: 61.05

Gene sequence:

>1050_bases
GTGAACAGTTTTTCCGGCTCGCTTGAAAAAGCGAAAACCCTGACGGAAAACAACGCCCCGATGGTGGACCCCTTCGGCCG
CGCCATCACCTATCTGCGCGTCTCCGTGACCGACCGCTGTGATTTTCGCTGCACCTACTGTATGTCCGAGCACATGACCT
TCCTGCCGAAGAAGGATCTGCTGACGCTCGAAGAACTCGACCGGCTCTGTTCCGTCTTCATAACGCGCGGCGTGCGCAAA
TTGCGGCTGACCGGCGGCGAGCCGCTGGTGCGCAAGAACATCATGTCGCTGGTGAGGAACCTTGGTCGGCATGTCCAAAG
CGGCACGCTTGACGAACTGACGCTGACCACCAACGGCTCGCAGCTTGCGAAATTCGCAGCCGAACTTGCCGATTGCGGGG
TGCGTCGCATCAATGTCTCGCTCGATACGCTCGACGCCCAGAAATTCCGCCAGATCACCCGCTGGGGCGATATCGACCGG
GTCATGGAAGGGTTTGATGCCGCGCAGGCAGCGGGCATCAAGGTCAAGCTCAACGCCGTGGCTCTGAAGGATTTCAACGA
CGCCGAAATGCCGGAACTGATGCGCTTTGCCCATGGCCGCGGCATGGACCTGACTGTCATCGAAACCATGCCGATGGGCG
AAATCGAGGAAGACCGCACCGACCGCTACCTGCCGCTCTCGCAGCTGCGCGCCGATCTCGAACGGAATTTCACCCTTGTC
GACAGCGATTACCAGACCGGCGGCCCCGCCCGTTACGTGACGGTGAAGGAAACCGGCGGCCGGCTCGGCTTCATTACGCC
GATGACCCATAATTTCTGCGAAAGCTGCAACCGCGTCCGCCTCACCTGCACCGGCACGCTTTATATGTGTCTCGGCCAGG
ACGATGCCGCCGATCTGCGCACGGCACTGCGCGCCTCCGACAGCGATGCCTATCTTTCCGCCGCCATCGATGAAGCGCTG
CTGCGCAAGCCGAAGGGGCATGATTTCATCATTGATCGCACCCACAACCGCCCCGCCGTCTCCCGCCATATGAGCGTGAC
TGGCGGCTGA

Upstream 100 bases:

>100_bases
GAATTACGGACTTGCCTCAGGATAGGCAAGCCACTACATCAGCTATAAGCCACATAGTCGGCCCCGTGTGTCCGGCGCGT
GACGCGGTGAGGATGGAGAC

Downstream 100 bases:

>100_bases
GGTCGGCTGAACTTCTTTCTCGTCCACCCACCCATATAAACGGTTGAATTGCCAGCTCTACCGTGCCACGTCTCTATGCA
TGGAGGAATGCGTGGTCGTG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNSFSGSLEKAKTLTENNAPMVDPFGRAITYLRVSVTDRCDFRCTYCMSEHMTFLPKKDLLTLEELDRLCSVFITRGVRK
LRLTGGEPLVRKNIMSLVRNLGRHVQSGTLDELTLTTNGSQLAKFAAELADCGVRRINVSLDTLDAQKFRQITRWGDIDR
VMEGFDAAQAAGIKVKLNAVALKDFNDAEMPELMRFAHGRGMDLTVIETMPMGEIEEDRTDRYLPLSQLRADLERNFTLV
DSDYQTGGPARYVTVKETGGRLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQDDAADLRTALRASDSDAYLSAAIDEAL
LRKPKGHDFIIDRTHNRPAVSRHMSVTGG

Sequences:

>Translated_349_residues
MNSFSGSLEKAKTLTENNAPMVDPFGRAITYLRVSVTDRCDFRCTYCMSEHMTFLPKKDLLTLEELDRLCSVFITRGVRK
LRLTGGEPLVRKNIMSLVRNLGRHVQSGTLDELTLTTNGSQLAKFAAELADCGVRRINVSLDTLDAQKFRQITRWGDIDR
VMEGFDAAQAAGIKVKLNAVALKDFNDAEMPELMRFAHGRGMDLTVIETMPMGEIEEDRTDRYLPLSQLRADLERNFTLV
DSDYQTGGPARYVTVKETGGRLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQDDAADLRTALRASDSDAYLSAAIDEAL
LRKPKGHDFIIDRTHNRPAVSRHMSVTGG
>Mature_349_residues
MNSFSGSLEKAKTLTENNAPMVDPFGRAITYLRVSVTDRCDFRCTYCMSEHMTFLPKKDLLTLEELDRLCSVFITRGVRK
LRLTGGEPLVRKNIMSLVRNLGRHVQSGTLDELTLTTNGSQLAKFAAELADCGVRRINVSLDTLDAQKFRQITRWGDIDR
VMEGFDAAQAAGIKVKLNAVALKDFNDAEMPELMRFAHGRGMDLTVIETMPMGEIEEDRTDRYLPLSQLRADLERNFTLV
DSDYQTGGPARYVTVKETGGRLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQDDAADLRTALRASDSDAYLSAAIDEAL
LRKPKGHDFIIDRTHNRPAVSRHMSVTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=335, Percent_Identity=34.9253731343284, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI22027536, Length=335, Percent_Identity=34.9253731343284, Blast_Score=187, Evalue=8e-48,
Organism=Escherichia coli, GI1786999, Length=334, Percent_Identity=32.9341317365269, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI32566331, Length=337, Percent_Identity=36.2017804154303, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17561210, Length=345, Percent_Identity=36.5217391304348, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI32566329, Length=338, Percent_Identity=36.6863905325444, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI28574488, Length=340, Percent_Identity=37.0588235294118, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28574490, Length=334, Percent_Identity=37.4251497005988, Blast_Score=208, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_AGRT5 (Q8UER0)

Other databases:

- EMBL:   AE007869
- PIR:   AI2784
- PIR:   B97564
- RefSeq:   NP_354682.1
- ProteinModelPortal:   Q8UER0
- SMR:   Q8UER0
- STRING:   Q8UER0
- GeneID:   1133733
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1695
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   FFRAMSR
- PhylomeDB:   Q8UER0
- ProtClustDB:   PRK00164
- BioCyc:   ATUM176299-1:ATU1695-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 38990; Mature: 38990

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 33-33 BINDING 46-46 BINDING 82-82 BINDING 86-86 BINDING 116-116 BINDING 140-140 BINDING 176-176 BINDING 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFSGSLEKAKTLTENNAPMVDPFGRAITYLRVSVTDRCDFRCTYCMSEHMTFLPKKDL
CCCCCCCHHHHHHHHCCCCCCCCCHHHEEEEEEEEECCCCCCEEEEEHHHHHHCCCCCCC
LTLEELDRLCSVFITRGVRKLRLTGGEPLVRKNIMSLVRNLGRHVQSGTLDELTLTTNGS
CCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHH
QLAKFAAELADCGVRRINVSLDTLDAQKFRQITRWGDIDRVMEGFDAAQAAGIKVKLNAV
HHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHCCHHHHCCEEEEEEEE
ALKDFNDAEMPELMRFAHGRGMDLTVIETMPMGEIEEDRTDRYLPLSQLRADLERNFTLV
EEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEE
DSDYQTGGPARYVTVKETGGRLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQDDAADLR
ECCCCCCCCEEEEEEEECCCEEEEECCCHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHH
TALRASDSDAYLSAAIDEALLRKPKGHDFIIDRTHNRPAVSRHMSVTGG
HHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCEECCCC
>Mature Secondary Structure
MNSFSGSLEKAKTLTENNAPMVDPFGRAITYLRVSVTDRCDFRCTYCMSEHMTFLPKKDL
CCCCCCCHHHHHHHHCCCCCCCCCHHHEEEEEEEEECCCCCCEEEEEHHHHHHCCCCCCC
LTLEELDRLCSVFITRGVRKLRLTGGEPLVRKNIMSLVRNLGRHVQSGTLDELTLTTNGS
CCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHH
QLAKFAAELADCGVRRINVSLDTLDAQKFRQITRWGDIDRVMEGFDAAQAAGIKVKLNAV
HHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHCCHHHHCCEEEEEEEE
ALKDFNDAEMPELMRFAHGRGMDLTVIETMPMGEIEEDRTDRYLPLSQLRADLERNFTLV
EEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEE
DSDYQTGGPARYVTVKETGGRLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQDDAADLR
ECCCCCCCCEEEEEEEECCCEEEEECCCHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHH
TALRASDSDAYLSAAIDEALLRKPKGHDFIIDRTHNRPAVSRHMSVTGG
HHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194