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The map label for this gene is mazG [H]

Identifier: 159184764

GI number: 159184764

Start: 1448103

End: 1448948

Strand: Reverse

Name: mazG [H]

Synonym: Atu1452

Alternate gene names: 159184764

Gene position: 1448948-1448103 (Counterclockwise)

Preceding gene: 159184765

Following gene: 159184762

Centisome position: 50.99

GC content: 59.1

Gene sequence:

>846_bases
ATGGAAGCCTCGAAGGATATTTCCCGCCTGATCGAGATCATGGAAGCCCTGCGCCAGCCGGAAACCGGCTGCCCCTGGGA
TGTCGTGCAGACCTTCGAGACGATCAAGCCCTATACGATCGAAGAGGCCTACGAGGTTGCCGACGCGATCGAGCGCAAGG
ATATGGACGATCTTTGCGATGAGCTGGGCGATCTGCTGTTGCAGGTGGTTTTCCATGCCCGCATTGCCGAGGAACGGGGT
GAATTCACCTTTGGTGATGTGGTGCACGCCGTCACCTCGAAGATGATCCGCCGCCATCCGCATGTCTTCGACGTCTCCGA
TGCCGACACGCCTGACAGCGTCAAGCTGCAATGGGACCAGATCAAGGCCGAGGAGAAGCGTGAAAGGGCAGAGCGCCGTG
CACGCCGGGGCATCACCGAAGATTTCAAGGCAGGGTTTCTGGGCGGCGTGCAGCGTAGCCAGCCGGCTCTGACCGAGGCG
CTCAAGTTGCAGGAACAGGCTGCGCGCGTCGGTTTTGACTGGTCGGACCCGGCTCCCATCCTCGACAAGATCGAGGAGGA
AATCGCCGAACTTCGCGAGGCGCTGGCGGAGGGAAAGCCGGAGAAAGTCTCTGACGAACTCGGCGATCTGATTTTTGCGC
TGGTCAATATCGGCCGCCACGTTAAAGCCGATCCGGAAGATGCGCTACGTGGCACCAATACGAAATTCCGCAGGCGTTTC
AATCACATCGAGACGTCGCTCAGCGACAATGGCGAGACGTTGAAAGAGGCGAGCCTCGAGAGGATGGAAGATTTGTGGCA
GGCGGCGAAACGGATCGAGCGTTCGCTTGATACGGTTTCTTCCTGA

Upstream 100 bases:

>100_bases
CGACAACGATATTCCGCTGAATTAAGCATTTCCAGGGAAAACTGCGTAGCGGTTTTCCGCCCGGACAATGCGTAATATTA
AAAATGAAGGTTTAGACACG

Downstream 100 bases:

>100_bases
CCACTCGGGACATGCATCAAAAGCAGGAGCGCTCAGCGCTTCGAGGATTGTGTGTAGATGCGAAGGCCCTACTCCGTCAT
GCTCGGGCCTGTCCCGGGCA

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MEASKDISRLIEIMEALRQPETGCPWDVVQTFETIKPYTIEEAYEVADAIERKDMDDLCDELGDLLLQVVFHARIAEERG
EFTFGDVVHAVTSKMIRRHPHVFDVSDADTPDSVKLQWDQIKAEEKRERAERRARRGITEDFKAGFLGGVQRSQPALTEA
LKLQEQAARVGFDWSDPAPILDKIEEEIAELREALAEGKPEKVSDELGDLIFALVNIGRHVKADPEDALRGTNTKFRRRF
NHIETSLSDNGETLKEASLERMEDLWQAAKRIERSLDTVSS

Sequences:

>Translated_281_residues
MEASKDISRLIEIMEALRQPETGCPWDVVQTFETIKPYTIEEAYEVADAIERKDMDDLCDELGDLLLQVVFHARIAEERG
EFTFGDVVHAVTSKMIRRHPHVFDVSDADTPDSVKLQWDQIKAEEKRERAERRARRGITEDFKAGFLGGVQRSQPALTEA
LKLQEQAARVGFDWSDPAPILDKIEEEIAELREALAEGKPEKVSDELGDLIFALVNIGRHVKADPEDALRGTNTKFRRRF
NHIETSLSDNGETLKEASLERMEDLWQAAKRIERSLDTVSS
>Mature_281_residues
MEASKDISRLIEIMEALRQPETGCPWDVVQTFETIKPYTIEEAYEVADAIERKDMDDLCDELGDLLLQVVFHARIAEERG
EFTFGDVVHAVTSKMIRRHPHVFDVSDADTPDSVKLQWDQIKAEEKRERAERRARRGITEDFKAGFLGGVQRSQPALTEA
LKLQEQAARVGFDWSDPAPILDKIEEEIAELREALAEGKPEKVSDELGDLIFALVNIGRHVKADPEDALRGTNTKFRRRF
NHIETSLSDNGETLKEASLERMEDLWQAAKRIERSLDTVSS

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=274, Percent_Identity=46.7153284671533, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 31975; Mature: 31975

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEASKDISRLIEIMEALRQPETGCPWDVVQTFETIKPYTIEEAYEVADAIERKDMDDLCD
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
ELGDLLLQVVFHARIAEERGEFTFGDVVHAVTSKMIRRHPHVFDVSDADTPDSVKLQWDQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEHHHH
IKAEEKRERAERRARRGITEDFKAGFLGGVQRSQPALTEALKLQEQAARVGFDWSDPAPI
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
LDKIEEEIAELREALAEGKPEKVSDELGDLIFALVNIGRHVKADPEDALRGTNTKFRRRF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHH
NHIETSLSDNGETLKEASLERMEDLWQAAKRIERSLDTVSS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEASKDISRLIEIMEALRQPETGCPWDVVQTFETIKPYTIEEAYEVADAIERKDMDDLCD
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
ELGDLLLQVVFHARIAEERGEFTFGDVVHAVTSKMIRRHPHVFDVSDADTPDSVKLQWDQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEHHHH
IKAEEKRERAERRARRGITEDFKAGFLGGVQRSQPALTEALKLQEQAARVGFDWSDPAPI
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
LDKIEEEIAELREALAEGKPEKVSDELGDLIFALVNIGRHVKADPEDALRGTNTKFRRRF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHH
NHIETSLSDNGETLKEASLERMEDLWQAAKRIERSLDTVSS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]