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The map label for this gene is ispDF

Identifier: 159184762

GI number: 159184762

Start: 1435677

End: 1436864

Strand: Reverse

Name: ispDF

Synonym: Atu1443

Alternate gene names: 159184762

Gene position: 1436864-1435677 (Counterclockwise)

Preceding gene: 159184764

Following gene: 15888767

Centisome position: 50.57

GC content: 63.64

Gene sequence:

>1188_bases
ATGAAATTCGGCATCGTCATCGTCGCCGCCGGACGCGGTGAAAGAGCAGGCTCACCCGAGGAAGGGCCCAAGCAATATCG
CCCGATCGGCGGCAGGGCCGTGATCGAGCACACATTGGCGACCTTCCTGCGCTGGAACGACGCCTGCCCGATCGTGGTCG
TCAGCCATGCCGACGATGCGGCCCTGCTGTCACCGATCCTTGCGCGGCTTGATGCCGGCGAGCGGATCACCACAGTCACG
GGCGGCGCCACACGTCAGCAATCGGTACTGGCCGGGCTTGAGGCACTCTCCTCGCACGGCCTTACCCATGTGATGATCCA
TGATGCCGTGCGGCCTTTCGTCGCCGCGGACATGCTGGAGCGTATCGCCGCGCTGCACCGGGCGGGCGCGGGTGGCGTTC
TGCCTGCCCTGCCCGTCACCGATACGCTGAAGCGCGGCCTGGAGGATCGCGTTGTGGAGACGGTTTCCCGGCAGGGACTG
TATGCGGCGCAGACACCGCAAAGTTTCAGCTACAGCGACATTCTTGACGCGCATCGTGCCGCCGCTGCTTCAGGCAAAAC
GGATTTCACCGATGACGCGTCGATTGCCGAATGGACGGGACTGACCGTGACGCTGACCGAAGGTTCGGTCGACAATGTGA
AGCTCACTCTCAAGCGGGATATCGCCATGGCTGACGAAAAGCTCTCCCACGCCTTGCCCGATGTGCGCACCGGCAATGGT
TACGACGTGCACCAGCTGGAGGCCGGCGACGGCGTGACGCTCTGCGGCATCTTCATCGAGCACGATCAGCGACTGAAAGG
CCATTCGGATGCTGATGTGGCGCTACATGCGCTGACGGATGCCCTGCTCGCCACCTGCGGTGCCGGTGATATCGGCGATC
ATTTCCCGCCTTCCGACCCGCAATGGAAGGGTGCGGCCTCGCGCATCTTCCTTGAACATGCGGCCAAGGTCGTGCGCGAC
AATGGCGGCACGATCATGAATGCCGATGTCTCGCTGATCGCCGAAGCGCCACGCATCGGTCCACATCGCCAAGCCATGCG
GGAAGCACTTTCGGACATGCTCGGCATTGCGCTGGAGCGCTGCTCGGTGAAGGCGACAACCAATGAGACGATCGGTTTCG
TCGGCCGCCGGGAAGGCATCGCGGCCATCGCCACCGCCACCGTCGTCTACCAAGGCAGACCGCTATGA

Upstream 100 bases:

>100_bases
CGTCTTATCGGGCTGGCCATCCGGGTTTTGAAACGCTAGAGCAGGACTGTCAACCGCTAAGGGTTGCACACAAAGCATAT
GACCAATGCAGGAAAGTACT

Downstream 100 bases:

>100_bases
GCCTTTTCCCCGGAGACATCGAAGAACTGGCACGGCGAATCATCACCGATTTCACCCCCCTTGGTCTGATGGTCTCGACG
GCGGAAAGCTGCACCGGAGG

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKFGIVIVAAGRGERAGSPEEGPKQYRPIGGRAVIEHTLATFLRWNDACPIVVVSHADDAALLSPILARLDAGERITTVT
GGATRQQSVLAGLEALSSHGLTHVMIHDAVRPFVAADMLERIAALHRAGAGGVLPALPVTDTLKRGLEDRVVETVSRQGL
YAAQTPQSFSYSDILDAHRAAAASGKTDFTDDASIAEWTGLTVTLTEGSVDNVKLTLKRDIAMADEKLSHALPDVRTGNG
YDVHQLEAGDGVTLCGIFIEHDQRLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWKGAASRIFLEHAAKVVRD
NGGTIMNADVSLIAEAPRIGPHRQAMREALSDMLGIALERCSVKATTNETIGFVGRREGIAAIATATVVYQGRPL

Sequences:

>Translated_395_residues
MKFGIVIVAAGRGERAGSPEEGPKQYRPIGGRAVIEHTLATFLRWNDACPIVVVSHADDAALLSPILARLDAGERITTVT
GGATRQQSVLAGLEALSSHGLTHVMIHDAVRPFVAADMLERIAALHRAGAGGVLPALPVTDTLKRGLEDRVVETVSRQGL
YAAQTPQSFSYSDILDAHRAAAASGKTDFTDDASIAEWTGLTVTLTEGSVDNVKLTLKRDIAMADEKLSHALPDVRTGNG
YDVHQLEAGDGVTLCGIFIEHDQRLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWKGAASRIFLEHAAKVVRD
NGGTIMNADVSLIAEAPRIGPHRQAMREALSDMLGIALERCSVKATTNETIGFVGRREGIAAIATATVVYQGRPL
>Mature_395_residues
MKFGIVIVAAGRGERAGSPEEGPKQYRPIGGRAVIEHTLATFLRWNDACPIVVVSHADDAALLSPILARLDAGERITTVT
GGATRQQSVLAGLEALSSHGLTHVMIHDAVRPFVAADMLERIAALHRAGAGGVLPALPVTDTLKRGLEDRVVETVSRQGL
YAAQTPQSFSYSDILDAHRAAAASGKTDFTDDASIAEWTGLTVTLTEGSVDNVKLTLKRDIAMADEKLSHALPDVRTGNG
YDVHQLEAGDGVTLCGIFIEHDQRLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWKGAASRIFLEHAAKVVRD
NGGTIMNADVSLIAEAPRIGPHRQAMREALSDMLGIALERCSVKATTNETIGFVGRREGIAAIATATVVYQGRPL

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=260, Percent_Identity=30.3846153846154, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=45.1612903225806, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=36.2445414847162, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_AGRT5 (Q8UFF4)

Other databases:

- EMBL:   AE007869
- PIR:   A97535
- PIR:   AC2754
- RefSeq:   NP_354449.2
- ProteinModelPortal:   Q8UFF4
- SMR:   Q8UFF4
- STRING:   Q8UFF4
- GeneID:   1133481
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1443
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q8UFF4
- ProtClustDB:   PRK09382
- BioCyc:   ATUM176299-1:ATU1443-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 41785; Mature: 41785

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFGIVIVAAGRGERAGSPEEGPKQYRPIGGRAVIEHTLATFLRWNDACPIVVVSHADDA
CCEEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCH
ALLSPILARLDAGERITTVTGGATRQQSVLAGLEALSSHGLTHVMIHDAVRPFVAADMLE
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHH
RIAALHRAGAGGVLPALPVTDTLKRGLEDRVVETVSRQGLYAAQTPQSFSYSDILDAHRA
HHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHH
AAASGKTDFTDDASIAEWTGLTVTLTEGSVDNVKLTLKRDIAMADEKLSHALPDVRTGNG
HHCCCCCCCCCCCCHHHHCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCC
YDVHQLEAGDGVTLCGIFIEHDQRLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDP
CCEEEEECCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QWKGAASRIFLEHAAKVVRDNGGTIMNADVSLIAEAPRIGPHRQAMREALSDMLGIALER
CCCCHHHHHHHHHHHHHHHCCCCEEEECCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHH
CSVKATTNETIGFVGRREGIAAIATATVVYQGRPL
HEEEECCCCCCCCCCCCCCHHHEEEEEEEECCCCC
>Mature Secondary Structure
MKFGIVIVAAGRGERAGSPEEGPKQYRPIGGRAVIEHTLATFLRWNDACPIVVVSHADDA
CCEEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCH
ALLSPILARLDAGERITTVTGGATRQQSVLAGLEALSSHGLTHVMIHDAVRPFVAADMLE
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHH
RIAALHRAGAGGVLPALPVTDTLKRGLEDRVVETVSRQGLYAAQTPQSFSYSDILDAHRA
HHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHH
AAASGKTDFTDDASIAEWTGLTVTLTEGSVDNVKLTLKRDIAMADEKLSHALPDVRTGNG
HHCCCCCCCCCCCCHHHHCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCC
YDVHQLEAGDGVTLCGIFIEHDQRLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDP
CCEEEEECCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QWKGAASRIFLEHAAKVVRDNGGTIMNADVSLIAEAPRIGPHRQAMREALSDMLGIALER
CCCCHHHHHHHHHHHHHHHCCCCEEEECCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHH
CSVKATTNETIGFVGRREGIAAIATATVVYQGRPL
HEEEECCCCCCCCCCCCCCHHHEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194