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The map label for this gene is rns [C]

Identifier: 159184629

GI number: 159184629

Start: 1122005

End: 1122763

Strand: Reverse

Name: rns [C]

Synonym: Atu1133

Alternate gene names: 159184629

Gene position: 1122763-1122005 (Counterclockwise)

Preceding gene: 159184630

Following gene: 15888469

Centisome position: 39.51

GC content: 61.4

Gene sequence:

>759_bases
ATGAAACGGTATGCAGGTTTCATCGCCTTGGGTCTTCTGTCGCTTGCAGCGCTGTGGTTTGCGCAGGAAAAACCCGTTTC
GAAGGGGCCGGAAAACGACCGGCAGACGCCCGCTCAGCAGCAATCCGCTGCACGCCCCCAACAGAATACAGCCCGGCAGA
ATGCGGACAATGCCCAACCTCCTCGTGGTACCGGCTTCGATTTCTACGTGCTCTCACTCTCCTGGTCGCCAGCCTTCTGT
GCCAGCTCCGAAGGTTCCGGCAACCGCCAGCAATGCGGCAGCGACAGGCGTTACGGTTTCGTGGTGCACGGGCTGTGGCC
GCAGAATGAAAACGGCTATCCCGAATTCTGCCAAAGCAGCGAGCCTGAGCGGGTTCCCGATGCGATCGGCCGCAGCATGT
TCGACATCATGCCATCGATGGGCCTGATTGGCCACCAGTGGCGTAAACACGGCGCCTGCTCCGGCCTGTCGCAGAAAGAT
TATTTTGCGGCAACACGGGCGGCGTTCGACGCCATCAGGTTGCCGGGGAAAATTTCCTCCGGCGCGCAGACCAATACGCT
CTCCGCCGATGCCATCGAAACCGCCTTCACCGACGCCAATCCGGGCCTGTCGAAACGCGCCGTCTCGATCAGCTGCGACG
GCAGGCGCCTTGAGGAAGTGCGCATCTGCCTCAACAAGGACATGGCATTTCGCGATTGCCCCGAACTGGACCGCAAGGGC
TGCCGCGCCAGCGCGACCGAGATCATCCCCATACGCTGA

Upstream 100 bases:

>100_bases
AACCTTTTTGCCCGTTTGCCTCGTTCACAAATTTATGTAAGACCGGATCGTCGCCCGACGGCGATATGGGGTGGTCCAGT
TTCCGGGGAACATGCACGGA

Downstream 100 bases:

>100_bases
TTACCCCATATCAACCCCATAGGACCCGACCATGGAACTGCTTTATTCGCCCGCCTCCCCCTACTCCGCCAAGGTGCGCA
TGGCCGCCCGCCATCTCGAC

Product: ribonuclease

Products: 3'- Phosphomononucleotides; 3'-Phosphooligonucleotides [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKRYAGFIALGLLSLAALWFAQEKPVSKGPENDRQTPAQQQSAARPQQNTARQNADNAQPPRGTGFDFYVLSLSWSPAFC
ASSEGSGNRQQCGSDRRYGFVVHGLWPQNENGYPEFCQSSEPERVPDAIGRSMFDIMPSMGLIGHQWRKHGACSGLSQKD
YFAATRAAFDAIRLPGKISSGAQTNTLSADAIETAFTDANPGLSKRAVSISCDGRRLEEVRICLNKDMAFRDCPELDRKG
CRASATEIIPIR

Sequences:

>Translated_252_residues
MKRYAGFIALGLLSLAALWFAQEKPVSKGPENDRQTPAQQQSAARPQQNTARQNADNAQPPRGTGFDFYVLSLSWSPAFC
ASSEGSGNRQQCGSDRRYGFVVHGLWPQNENGYPEFCQSSEPERVPDAIGRSMFDIMPSMGLIGHQWRKHGACSGLSQKD
YFAATRAAFDAIRLPGKISSGAQTNTLSADAIETAFTDANPGLSKRAVSISCDGRRLEEVRICLNKDMAFRDCPELDRKG
CRASATEIIPIR
>Mature_252_residues
MKRYAGFIALGLLSLAALWFAQEKPVSKGPENDRQTPAQQQSAARPQQNTARQNADNAQPPRGTGFDFYVLSLSWSPAFC
ASSEGSGNRQQCGSDRRYGFVVHGLWPQNENGYPEFCQSSEPERVPDAIGRSMFDIMPSMGLIGHQWRKHGACSGLSQKD
YFAATRAAFDAIRLPGKISSGAQTNTLSADAIETAFTDANPGLSKRAVSISCDGRRLEEVRICLNKDMAFRDCPELDRKG
CRASATEIIPIR

Specific function: One Of The Few RNAses That Cleaves The Phosphodiester Bond Between Any Two Nucleotide. Shows A Preference For Cytidylic Or Guanylic Acid. [C]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasmic Protein And Cytoplasm. [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17562468, Length=209, Percent_Identity=24.8803827751196, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: 3.1.27.6 [C]

Molecular weight: Translated: 27611; Mature: 27611

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYAGFIALGLLSLAALWFAQEKPVSKGPENDRQTPAQQQSAARPQQNTARQNADNAQP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHCCCCCCCC
PRGTGFDFYVLSLSWSPAFCASSEGSGNRQQCGSDRRYGFVVHGLWPQNENGYPEFCQSS
CCCCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCCCCEEEEEEEECCCCCCCCHHHHCCC
EPERVPDAIGRSMFDIMPSMGLIGHQWRKHGACSGLSQKDYFAATRAAFDAIRLPGKISS
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GAQTNTLSADAIETAFTDANPGLSKRAVSISCDGRRLEEVRICLNKDMAFRDCPELDRKG
CCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHCC
CRASATEIIPIR
CCCCCCEEEECC
>Mature Secondary Structure
MKRYAGFIALGLLSLAALWFAQEKPVSKGPENDRQTPAQQQSAARPQQNTARQNADNAQP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHCCCCCCCC
PRGTGFDFYVLSLSWSPAFCASSEGSGNRQQCGSDRRYGFVVHGLWPQNENGYPEFCQSS
CCCCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCCCCEEEEEEEECCCCCCCCHHHHCCC
EPERVPDAIGRSMFDIMPSMGLIGHQWRKHGACSGLSQKDYFAATRAAFDAIRLPGKISS
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GAQTNTLSADAIETAFTDANPGLSKRAVSISCDGRRLEEVRICLNKDMAFRDCPELDRKG
CCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHCC
CRASATEIIPIR
CCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nucleotides [C]

Specific reaction: Endonucleolytic Cleavage With 2',3'-Cyclic Phosphate Intermediates. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]