| Definition | Agrobacterium tumefaciens str. C58 chromosome circular, complete sequence. |
|---|---|
| Accession | NC_003062 |
| Length | 2,841,580 |
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The map label for this gene is dkgB [H]
Identifier: 159184506
GI number: 159184506
Start: 863796
End: 864623
Strand: Reverse
Name: dkgB [H]
Synonym: Atu0867
Alternate gene names: 159184506
Gene position: 864623-863796 (Counterclockwise)
Preceding gene: 15888211
Following gene: 15888204
Centisome position: 30.43
GC content: 58.94
Gene sequence:
>828_bases ATGTACGACGTCAGCGCGAATGGCGCGAATATTCCCGCCCTCGGTTTCGGCACCTTCCGCATTCCGGAAGACGATGTCAG GCGCATTCTGCCGGAAGCGCTGAAACTTGGTTTCCGCCATGTCGATACCGCCCAGATCTACAAGAACGAGGGTGCCGTCG GCGAGGTTATCGCGCAATCGGGCATTTCCCGGCACGATATCTTTCTCACCACCAAAGTCTGGGTGGACCGCTACCGCCAT GACGATTTTCTCGCCTCCGTCGATGAAAGCCTTGCGAAACTGAAGACCGACTATTTCGACCTGCTGCTTCTGCACTGGCC GAAAAGTGACGTGCCGCTTGCCGAGCGCATGGGCGCGTTGAATGCGGTGCGCAAGGCCGGCAAGGCCCGCAATATCGGCA TTTCCAACTTCAACGTGGCGCTGACCGAAGAAGCGGTGAAACTTTCCGACACGCCGCTTGCCACCAACCAGATCGAATAT CACCCCTATCTCGACCAGACGAAGGTGATTTCGGCGGCCCGTAAACACGGCATGTCGGTGACGGCCTATTATCTGATGGC CGATGGCAAGGTGCCGAACGATCCCGTTCTCAAGGATATCGGCGCGAAACACGGCAAGACCGCCGCACAGGTGACCCTGC GCTGGGCCGTGCAGCAGCCGGATATCGTGGCGCTTTCCAAGACGGCGACGGAAAGCCGCCTCAAGGAGAATTTCGACATT TTCGATTTTGCGCTCTCGGAAGAGGAAATGCAGGCGATCCATGCGCTTGCCAAGCCGAATGGCCGCATCGTCAGCCCCGG CCACCTCGCGCCCGATTGGGACGACTGA
Upstream 100 bases:
>100_bases GCGGTTGAAACCGATCGCGCCGACACCAGTTAAAGAAAGACTGCTTCCCCTAACCGGCCTCTTCCGCCGGCCATGTTTTC AGCAAACAGGAGAGACACCC
Downstream 100 bases:
>100_bases GGCGGCCGGCCATCCGGTCCGGTCTCAACGCTCGGTAAAGGCGAGCTTCGCCCCCAGCGCCACGAAAGCGGCGGCGAAGC TGCGCCTCAACCATGCGAGA
Product: aldo/keto reductase
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 275; Mature: 275
Protein sequence:
>275_residues MYDVSANGANIPALGFGTFRIPEDDVRRILPEALKLGFRHVDTAQIYKNEGAVGEVIAQSGISRHDIFLTTKVWVDRYRH DDFLASVDESLAKLKTDYFDLLLLHWPKSDVPLAERMGALNAVRKAGKARNIGISNFNVALTEEAVKLSDTPLATNQIEY HPYLDQTKVISAARKHGMSVTAYYLMADGKVPNDPVLKDIGAKHGKTAAQVTLRWAVQQPDIVALSKTATESRLKENFDI FDFALSEEEMQAIHALAKPNGRIVSPGHLAPDWDD
Sequences:
>Translated_275_residues MYDVSANGANIPALGFGTFRIPEDDVRRILPEALKLGFRHVDTAQIYKNEGAVGEVIAQSGISRHDIFLTTKVWVDRYRH DDFLASVDESLAKLKTDYFDLLLLHWPKSDVPLAERMGALNAVRKAGKARNIGISNFNVALTEEAVKLSDTPLATNQIEY HPYLDQTKVISAARKHGMSVTAYYLMADGKVPNDPVLKDIGAKHGKTAAQVTLRWAVQQPDIVALSKTATESRLKENFDI FDFALSEEEMQAIHALAKPNGRIVSPGHLAPDWDD >Mature_275_residues MYDVSANGANIPALGFGTFRIPEDDVRRILPEALKLGFRHVDTAQIYKNEGAVGEVIAQSGISRHDIFLTTKVWVDRYRH DDFLASVDESLAKLKTDYFDLLLLHWPKSDVPLAERMGALNAVRKAGKARNIGISNFNVALTEEAVKLSDTPLATNQIEY HPYLDQTKVISAARKHGMSVTAYYLMADGKVPNDPVLKDIGAKHGKTAAQVTLRWAVQQPDIVALSKTATESRLKENFDI FDFALSEEEMQAIHALAKPNGRIVSPGHLAPDWDD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI24497577, Length=294, Percent_Identity=31.2925170068027, Blast_Score=165, Evalue=5e-41, Organism=Homo sapiens, GI5174391, Length=294, Percent_Identity=31.2925170068027, Blast_Score=165, Evalue=5e-41, Organism=Homo sapiens, GI310109922, Length=273, Percent_Identity=34.0659340659341, Blast_Score=159, Evalue=2e-39, Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=32.5342465753425, Blast_Score=155, Evalue=5e-38, Organism=Homo sapiens, GI5174695, Length=300, Percent_Identity=32.6666666666667, Blast_Score=154, Evalue=6e-38, Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=32.1917808219178, Blast_Score=154, Evalue=1e-37, Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=32.1917808219178, Blast_Score=154, Evalue=1e-37, Organism=Homo sapiens, GI223468663, Length=284, Percent_Identity=31.3380281690141, Blast_Score=153, Evalue=1e-37, Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=32.1917808219178, Blast_Score=153, Evalue=2e-37, Organism=Homo sapiens, GI24497585, Length=298, Percent_Identity=31.8791946308725, Blast_Score=151, Evalue=8e-37, Organism=Homo sapiens, GI24497583, Length=290, Percent_Identity=31.7241379310345, Blast_Score=147, Evalue=1e-35, Organism=Homo sapiens, GI4502049, Length=295, Percent_Identity=33.2203389830508, Blast_Score=145, Evalue=3e-35, Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=30.7692307692308, Blast_Score=143, Evalue=1e-34, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=32.3741007194245, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI291291012, Length=264, Percent_Identity=31.4393939393939, Blast_Score=131, Evalue=7e-31, Organism=Homo sapiens, GI300116273, Length=276, Percent_Identity=29.7101449275362, Blast_Score=130, Evalue=2e-30, Organism=Homo sapiens, GI310109924, Length=153, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=4e-18, Organism=Homo sapiens, GI310109926, Length=121, Percent_Identity=36.3636363636364, Blast_Score=81, Evalue=1e-15, Organism=Homo sapiens, GI207028673, Length=121, Percent_Identity=35.5371900826446, Blast_Score=80, Evalue=2e-15, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=45.6928838951311, Blast_Score=228, Evalue=4e-61, Organism=Escherichia coli, GI87082198, Length=263, Percent_Identity=31.5589353612167, Blast_Score=154, Evalue=9e-39, Organism=Escherichia coli, GI1788081, Length=270, Percent_Identity=30.7407407407407, Blast_Score=111, Evalue=4e-26, Organism=Escherichia coli, GI87081735, Length=297, Percent_Identity=26.2626262626263, Blast_Score=83, Evalue=2e-17, Organism=Escherichia coli, GI1787674, Length=265, Percent_Identity=23.7735849056604, Blast_Score=67, Evalue=9e-13, Organism=Escherichia coli, GI1789375, Length=311, Percent_Identity=21.5434083601286, Blast_Score=64, Evalue=1e-11, Organism=Caenorhabditis elegans, GI17537077, Length=292, Percent_Identity=32.5342465753425, Blast_Score=156, Evalue=1e-38, Organism=Caenorhabditis elegans, GI17537075, Length=294, Percent_Identity=32.312925170068, Blast_Score=156, Evalue=1e-38, Organism=Caenorhabditis elegans, GI17561298, Length=280, Percent_Identity=32.1428571428571, Blast_Score=154, Evalue=4e-38, Organism=Caenorhabditis elegans, GI17550248, Length=277, Percent_Identity=33.2129963898917, Blast_Score=146, Evalue=1e-35, Organism=Caenorhabditis elegans, GI17537079, Length=299, Percent_Identity=28.7625418060201, Blast_Score=145, Evalue=2e-35, Organism=Caenorhabditis elegans, GI17566692, Length=284, Percent_Identity=30.6338028169014, Blast_Score=140, Evalue=6e-34, Organism=Caenorhabditis elegans, GI17552492, Length=266, Percent_Identity=28.5714285714286, Blast_Score=137, Evalue=7e-33, Organism=Caenorhabditis elegans, GI71998625, Length=275, Percent_Identity=29.4545454545455, Blast_Score=137, Evalue=8e-33, Organism=Caenorhabditis elegans, GI17564128, Length=289, Percent_Identity=30.4498269896194, Blast_Score=131, Evalue=5e-31, Organism=Caenorhabditis elegans, GI17561300, Length=266, Percent_Identity=30.8270676691729, Blast_Score=120, Evalue=5e-28, Organism=Caenorhabditis elegans, GI17538386, Length=268, Percent_Identity=30.2238805970149, Blast_Score=117, Evalue=7e-27, Organism=Caenorhabditis elegans, GI17562292, Length=277, Percent_Identity=28.5198555956679, Blast_Score=114, Evalue=8e-26, Organism=Saccharomyces cerevisiae, GI6324694, Length=296, Percent_Identity=34.7972972972973, Blast_Score=157, Evalue=1e-39, Organism=Saccharomyces cerevisiae, GI6320576, Length=285, Percent_Identity=34.3859649122807, Blast_Score=155, Evalue=5e-39, Organism=Saccharomyces cerevisiae, GI6321896, Length=299, Percent_Identity=28.4280936454849, Blast_Score=124, Evalue=2e-29, Organism=Saccharomyces cerevisiae, GI6322556, Length=261, Percent_Identity=30.2681992337165, Blast_Score=122, Evalue=8e-29, Organism=Saccharomyces cerevisiae, GI6319625, Length=287, Percent_Identity=28.9198606271777, Blast_Score=113, Evalue=3e-26, Organism=Saccharomyces cerevisiae, GI6320079, Length=279, Percent_Identity=31.1827956989247, Blast_Score=108, Evalue=6e-25, Organism=Drosophila melanogaster, GI24657054, Length=282, Percent_Identity=33.6879432624113, Blast_Score=149, Evalue=2e-36, Organism=Drosophila melanogaster, GI24662789, Length=296, Percent_Identity=29.3918918918919, Blast_Score=147, Evalue=9e-36, Organism=Drosophila melanogaster, GI45553081, Length=278, Percent_Identity=32.7338129496403, Blast_Score=142, Evalue=2e-34, Organism=Drosophila melanogaster, GI20129731, Length=276, Percent_Identity=32.9710144927536, Blast_Score=142, Evalue=4e-34, Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=30.7692307692308, Blast_Score=141, Evalue=5e-34, Organism=Drosophila melanogaster, GI24663317, Length=279, Percent_Identity=30.8243727598566, Blast_Score=140, Evalue=1e-33, Organism=Drosophila melanogaster, GI24662785, Length=294, Percent_Identity=33.6734693877551, Blast_Score=139, Evalue=2e-33, Organism=Drosophila melanogaster, GI21356425, Length=276, Percent_Identity=32.6086956521739, Blast_Score=137, Evalue=6e-33, Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=28.2131661442006, Blast_Score=136, Evalue=1e-32, Organism=Drosophila melanogaster, GI24662781, Length=291, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=1e-32, Organism=Drosophila melanogaster, GI281366140, Length=249, Percent_Identity=32.9317269076305, Blast_Score=124, Evalue=1e-28,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 30479; Mature: 30479
Theoretical pI: Translated: 6.43; Mature: 6.43
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYDVSANGANIPALGFGTFRIPEDDVRRILPEALKLGFRHVDTAQIYKNEGAVGEVIAQS CCCCCCCCCCCCEECCCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHC GISRHDIFLTTKVWVDRYRHDDFLASVDESLAKLKTDYFDLLLLHWPKSDVPLAERMGAL CCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHH NAVRKAGKARNIGISNFNVALTEEAVKLSDTPLATNQIEYHPYLDQTKVISAARKHGMSV HHHHHCCCCCCCCCCCCEEEEEHHHHEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCEE TAYYLMADGKVPNDPVLKDIGAKHGKTAAQVTLRWAVQQPDIVALSKTATESRLKENFDI EEEEEEECCCCCCCCHHHHHCCCCCCHHHEEEEEEEECCCCEEEEECHHHHHHHHHCCCC FDFALSEEEMQAIHALAKPNGRIVSPGHLAPDWDD EEECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCC >Mature Secondary Structure MYDVSANGANIPALGFGTFRIPEDDVRRILPEALKLGFRHVDTAQIYKNEGAVGEVIAQS CCCCCCCCCCCCEECCCEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHC GISRHDIFLTTKVWVDRYRHDDFLASVDESLAKLKTDYFDLLLLHWPKSDVPLAERMGAL CCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHH NAVRKAGKARNIGISNFNVALTEEAVKLSDTPLATNQIEYHPYLDQTKVISAARKHGMSV HHHHHCCCCCCCCCCCCEEEEEHHHHEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCEE TAYYLMADGKVPNDPVLKDIGAKHGKTAAQVTLRWAVQQPDIVALSKTATESRLKENFDI EEEEEEECCCCCCCCHHHHHCCCCCCHHHEEEEEEEECCCCEEEEECHHHHHHHHHCCCC FDFALSEEEMQAIHALAKPNGRIVSPGHLAPDWDD EEECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796; 11108008 [H]