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The map label for this gene is tam

Identifier: 15888211

GI number: 15888211

Start: 866012

End: 866782

Strand: Reverse

Name: tam

Synonym: Atu0870

Alternate gene names: 15888211

Gene position: 866782-866012 (Counterclockwise)

Preceding gene: 159184509

Following gene: 159184506

Centisome position: 30.5

GC content: 61.48

Gene sequence:

>771_bases
ATGGCGTGGTCCGCGCAACAATATCTGAAATTCGAAGACGAACGGACGCGCCCCGCGCGGGATCTTCTGGCCCAGGTGCC
GCTGGAGCGCGTGCTGAATGGTTATGACCTCGGCTGCGGCCCGGGCAATTCCACCGAGCTTCTGACCGACCGTTACGGCG
TCAACGTCATCACCGGCATCGACAGCGACGACGACATGCTGGAAAAGGCGGCGGACCGGCTACCGAATACCAATTTCGGC
AAGGCCGACCTTGCGACGTGGAAACCGGCGCAGAAGGCGGACCTGCTTTACGCCAATGCCGTTTTCCAGTGGGTGCCGGA
CCATCTTGCCGTTCTGTCACAACTGATGGACCAGCTGGAAAGCGGCGGTGTTCTCGCCGTGCAGATGCCTGACAATCTGC
AGGAGCCGACGCATATCGCGATGCATGAAACGGCCGACGGGGGACCGTGGAAGGACGCTTTTTCCGGTGGCGGCCTGCGT
CGCAAACCTCTTCCCCCACCCTCGGACTATTTTAATGCGCTTTCGCCGAAATCCTCGCGGGTGGATGTCTGGCACACCGT
CTATAATCACCCGATGAAGGATGCGGACAGCATTGTCGAATGGGTGAAGGGCACGGGGCTGCGCCCCTATCTCGCCGCCG
CAGGCGAGGAAAACCGCGAAGCCTTCCTCGCCGATTATACGCGACGCATCGCGGCGGCCTATCCTCCCATGGCGGACGGC
CGGTTGCTGCTGCGTTTTCCCCGGCTTTTCGTGGTGGCGGTGAAGAAATAG

Upstream 100 bases:

>100_bases
GATGAATAGAATCATCACAATAATTCATTGATCCGATGATGGCGGGGCCGTTTCGGCTATGCGATGCTGACGATCTGACG
GCAGTCCGCAGGAGGAAATC

Downstream 100 bases:

>100_bases
GGTTATCAACCCGCGAAGGCATAGACCCGGAGGCGGTGGCTGTCTGGGTCGGCGGCCGTGAAGGTATAACCGAACTCCAT
TTTCGCGGGCTGCTGCAGCA

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAWSAQQYLKFEDERTRPARDLLAQVPLERVLNGYDLGCGPGNSTELLTDRYGVNVITGIDSDDDMLEKAADRLPNTNFG
KADLATWKPAQKADLLYANAVFQWVPDHLAVLSQLMDQLESGGVLAVQMPDNLQEPTHIAMHETADGGPWKDAFSGGGLR
RKPLPPPSDYFNALSPKSSRVDVWHTVYNHPMKDADSIVEWVKGTGLRPYLAAAGEENREAFLADYTRRIAAAYPPMADG
RLLLRFPRLFVVAVKK

Sequences:

>Translated_256_residues
MAWSAQQYLKFEDERTRPARDLLAQVPLERVLNGYDLGCGPGNSTELLTDRYGVNVITGIDSDDDMLEKAADRLPNTNFG
KADLATWKPAQKADLLYANAVFQWVPDHLAVLSQLMDQLESGGVLAVQMPDNLQEPTHIAMHETADGGPWKDAFSGGGLR
RKPLPPPSDYFNALSPKSSRVDVWHTVYNHPMKDADSIVEWVKGTGLRPYLAAAGEENREAFLADYTRRIAAAYPPMADG
RLLLRFPRLFVVAVKK
>Mature_255_residues
AWSAQQYLKFEDERTRPARDLLAQVPLERVLNGYDLGCGPGNSTELLTDRYGVNVITGIDSDDDMLEKAADRLPNTNFGK
ADLATWKPAQKADLLYANAVFQWVPDHLAVLSQLMDQLESGGVLAVQMPDNLQEPTHIAMHETADGGPWKDAFSGGGLRR
KPLPPPSDYFNALSPKSSRVDVWHTVYNHPMKDADSIVEWVKGTGLRPYLAAAGEENREAFLADYTRRIAAAYPPMADGR
LLLRFPRLFVVAVKK

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family

Homologues:

Organism=Escherichia coli, GI1787798, Length=251, Percent_Identity=45.8167330677291, Blast_Score=225, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TAM_AGRT5 (Q8UH15)

Other databases:

- EMBL:   AE007869
- PIR:   AF2683
- PIR:   D97465
- RefSeq:   NP_353892.1
- PDB:   2P35
- PDBsum:   2P35
- ProteinModelPortal:   Q8UH15
- SMR:   Q8UH15
- STRING:   Q8UH15
- GeneID:   1132908
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0870
- eggNOG:   COG4106
- HOGENOM:   HBG328684
- OMA:   WETTYVH
- PhylomeDB:   Q8UH15
- ProtClustDB:   PRK01683
- BioCyc:   ATUM176299-1:ATU0870-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00560
- InterPro:   IPR013216
- InterPro:   IPR023149

Pfam domain/function: PF08241 Methyltransf_11

EC number: =2.1.1.144

Molecular weight: Translated: 28437; Mature: 28306

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWSAQQYLKFEDERTRPARDLLAQVPLERVLNGYDLGCGPGNSTELLTDRYGVNVITGI
CCCCHHHHHHCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEHHCCCCEEEECC
DSDDDMLEKAADRLPNTNFGKADLATWKPAQKADLLYANAVFQWVPDHLAVLSQLMDQLE
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SGGVLAVQMPDNLQEPTHIAMHETADGGPWKDAFSGGGLRRKPLPPPSDYFNALSPKSSR
CCCEEEEECCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCCCC
VDVWHTVYNHPMKDADSIVEWVKGTGLRPYLAAAGEENREAFLADYTRRIAAAYPPMADG
HHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
RLLLRFPRLFVVAVKK
CHHEEECCEEEEEECC
>Mature Secondary Structure 
AWSAQQYLKFEDERTRPARDLLAQVPLERVLNGYDLGCGPGNSTELLTDRYGVNVITGI
CCCHHHHHHCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEHHCCCCEEEECC
DSDDDMLEKAADRLPNTNFGKADLATWKPAQKADLLYANAVFQWVPDHLAVLSQLMDQLE
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SGGVLAVQMPDNLQEPTHIAMHETADGGPWKDAFSGGGLRRKPLPPPSDYFNALSPKSSR
CCCEEEEECCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCCCC
VDVWHTVYNHPMKDADSIVEWVKGTGLRPYLAAAGEENREAFLADYTRRIAAAYPPMADG
HHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
RLLLRFPRLFVVAVKK
CHHEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194