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The map label for this gene is iunH [H]

Identifier: 159184279

GI number: 159184279

Start: 369370

End: 370314

Strand: Reverse

Name: iunH [H]

Synonym: Atu0374

Alternate gene names: 159184279

Gene position: 370314-369370 (Counterclockwise)

Preceding gene: 159184280

Following gene: 159184277

Centisome position: 13.03

GC content: 60.11

Gene sequence:

>945_bases
ATGGCAGAACGCAGAAAAATCATCATCGACACCGATCCGGGCCAGGACGACGCGGCGGCAATCATGCTCGCCTTTGGAAG
TCCTGAGGAAATCGACATTCTCGGACTGTGCGCCGTTGCCGGCAACGTTCCGCTGAAGCTGACCAGCCGCAACATCCGCA
TCATCTGCGAATTGTGCGGCCGCACCGATATTCCCGTCTATGAGGGCGCGGAGAAGCCGCTGGTGCGCAAGCCCATCACC
GCCGAGCATGTTCACGGCAGCACCGGCCTTAACGGCCCGGTTCTCGATGAACCGACCATGGAGGCGCAAAAGCAGCATGC
GGTCGATTTCATCATCGAAACGCTGATGCGGGAACCCGCCGGCACCGTAACGCTCTGCACGCTTGGCGCGCTGACCAATG
TGGCGCTGGCTCTCCTCAAGGCGCCGGAAATTGCCGATCGCGTCAAGGAACTGGTGATGATGGGCGGCGGCTTCTTCGAA
GGTGGAAACATCACGCCGGCGGCGGAATTCAACATCTATGTCGACCCGCAGGCCGCCGACATCGTTTTCCGTTCCGGCAT
GCCTGTCGTCATGATGCCGCTCGACGTCACCCACAAGCTGCTGACCACCAAGGCGCGCGTCAACCGCATTCGTGACATTG
GCACGCGCCCTGCCATCGCCATGGCGGAAATGCTGGAATTCTTCGAGCGTTTTGACATCGAGAAATACGGTTCGGATGGC
GGCCCGCTGCATGATCCGAGCGTCATTGCCTATCTCCTCAAGCCCGAACTGTTCGAGGGCCGCGAGTGCAATGTCGAAAT
CGAGGTCATTTCCGAACTGACCATGGGCATGACTGTCGTGGACTGGTGGCGGGTGACTGACCGTCCCGCCAATGCCCGCG
TGATGCGCAATGTGGATGCCGACGGCTTCTTTGAATTGCTGACCGAACGTTTCGCCCGGCTCTGA

Upstream 100 bases:

>100_bases
CGCAAGACGAAACGCGGGCAAAGGCAGGAGGGAGGAATGAGGCTTGACAAGTGAGCACCCGCCGCTCGAAAAAAGCGCTC
ATCTCAACGGCGGGCAAGAC

Downstream 100 bases:

>100_bases
TCCGGACAGGAACAAAAAAAGGCCGCTGGAAAAGCCAGCGGCCCTTTTATTTTCAGCGGGAATGGCGGATCAGAATTCCG
TCCAGTCATCCTGCGCCAGC

Product: inosine-uridine preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MAERRKIIIDTDPGQDDAAAIMLAFGSPEEIDILGLCAVAGNVPLKLTSRNIRIICELCGRTDIPVYEGAEKPLVRKPIT
AEHVHGSTGLNGPVLDEPTMEAQKQHAVDFIIETLMREPAGTVTLCTLGALTNVALALLKAPEIADRVKELVMMGGGFFE
GGNITPAAEFNIYVDPQAADIVFRSGMPVVMMPLDVTHKLLTTKARVNRIRDIGTRPAIAMAEMLEFFERFDIEKYGSDG
GPLHDPSVIAYLLKPELFEGRECNVEIEVISELTMGMTVVDWWRVTDRPANARVMRNVDADGFFELLTERFARL

Sequences:

>Translated_314_residues
MAERRKIIIDTDPGQDDAAAIMLAFGSPEEIDILGLCAVAGNVPLKLTSRNIRIICELCGRTDIPVYEGAEKPLVRKPIT
AEHVHGSTGLNGPVLDEPTMEAQKQHAVDFIIETLMREPAGTVTLCTLGALTNVALALLKAPEIADRVKELVMMGGGFFE
GGNITPAAEFNIYVDPQAADIVFRSGMPVVMMPLDVTHKLLTTKARVNRIRDIGTRPAIAMAEMLEFFERFDIEKYGSDG
GPLHDPSVIAYLLKPELFEGRECNVEIEVISELTMGMTVVDWWRVTDRPANARVMRNVDADGFFELLTERFARL
>Mature_313_residues
AERRKIIIDTDPGQDDAAAIMLAFGSPEEIDILGLCAVAGNVPLKLTSRNIRIICELCGRTDIPVYEGAEKPLVRKPITA
EHVHGSTGLNGPVLDEPTMEAQKQHAVDFIIETLMREPAGTVTLCTLGALTNVALALLKAPEIADRVKELVMMGGGFFEG
GNITPAAEFNIYVDPQAADIVFRSGMPVVMMPLDVTHKLLTTKARVNRIRDIGTRPAIAMAEMLEFFERFDIEKYGSDGG
PLHDPSVIAYLLKPELFEGRECNVEIEVISELTMGMTVVDWWRVTDRPANARVMRNVDADGFFELLTERFARL

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=306, Percent_Identity=49.6732026143791, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=42.0195439739414, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1786213, Length=311, Percent_Identity=38.9067524115756, Blast_Score=164, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI32565474, Length=200, Percent_Identity=35.5, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17565698, Length=315, Percent_Identity=29.2063492063492, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320608, Length=260, Percent_Identity=34.6153846153846, Blast_Score=136, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24641837, Length=333, Percent_Identity=29.4294294294294, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24583915, Length=205, Percent_Identity=29.7560975609756, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641839, Length=329, Percent_Identity=27.355623100304, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34581; Mature: 34449

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERRKIIIDTDPGQDDAAAIMLAFGSPEEIDILGLCAVAGNVPLKLTSRNIRIICELCG
CCCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCEEEEEEECC
RTDIPVYEGAEKPLVRKPITAEHVHGSTGLNGPVLDEPTMEAQKQHAVDFIIETLMREPA
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GTVTLCTLGALTNVALALLKAPEIADRVKELVMMGGGFFEGGNITPAAEFNIYVDPQAAD
CCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEECCCHHE
IVFRSGMPVVMMPLDVTHKLLTTKARVNRIRDIGTRPAIAMAEMLEFFERFDIEKYGSDG
EEEECCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCCC
GPLHDPSVIAYLLKPELFEGRECNVEIEVISELTMGMTVVDWWRVTDRPANARVMRNVDA
CCCCCHHHHHEECCCCCCCCCCCCEEEEEHHHHHCCCEEEEEEECCCCCCCCCEEECCCC
DGFFELLTERFARL
HHHHHHHHHHHHCC
>Mature Secondary Structure 
AERRKIIIDTDPGQDDAAAIMLAFGSPEEIDILGLCAVAGNVPLKLTSRNIRIICELCG
CCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCEEEEEEECC
RTDIPVYEGAEKPLVRKPITAEHVHGSTGLNGPVLDEPTMEAQKQHAVDFIIETLMREPA
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GTVTLCTLGALTNVALALLKAPEIADRVKELVMMGGGFFEGGNITPAAEFNIYVDPQAAD
CCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEECCCHHE
IVFRSGMPVVMMPLDVTHKLLTTKARVNRIRDIGTRPAIAMAEMLEFFERFDIEKYGSDG
EEEECCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCCC
GPLHDPSVIAYLLKPELFEGRECNVEIEVISELTMGMTVVDWWRVTDRPANARVMRNVDA
CCCCCHHHHHEECCCCCCCCCCCCEEEEEHHHHHCCCEEEEEEECCCCCCCCCEEECCCC
DGFFELLTERFARL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA