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The map label for this gene is recF

Identifier: 159184149

GI number: 159184149

Start: 76045

End: 77172

Strand: Direct

Name: recF

Synonym: Atu0073

Alternate gene names: 159184149

Gene position: 76045-77172 (Clockwise)

Preceding gene: 159184148

Following gene: 159184150

Centisome position: 2.68

GC content: 61.52

Gene sequence:

>1128_bases
ATGACGAACAAGGTGTCGCTTTCCCGACTGAAACTTACGGACTTCCGCAACTATGCGGCTGCCGCACTTGTGCTTGATGA
GCGCCATGTGGTGCTGACCGGCGACAACGGTTCCGGCAAGACCAACCTTCTGGAGGCGGTTTCGTTTCTTTCGCCGGGAC
GCGGCCTGCGCCGTGCGGTCCTGTCCGACGTTACGCGCGTTGGCGCGGAAGCCACCGGTTTTTCGATCTTCGCCGATGTT
GATGGCATGGATGGCGAAGTCGCCATCGGCACCGGGATCGAGGGTGATGGCGAGGTAGTGTCACGTCGCCTGCGGCTGAA
CGGTACGCCGGTGAAGTCCGTCGATGAATTGACCGACCATCTGCGGGTTCTGTGGCTGACGCCCGCCATGGATGGCCTCT
TTACCGGTTCTTCATCGGACCGCCGGCGTTTTCTCGATCGGTTGGTCCTGTCGCTCGATCCGGGCCACGGCCGGCGGGCA
AGCGATTTCGAAAAAGCCATGCGCGGCCGCAACCGCCTGCTTTCGGAAGGCCGTTTTGATCCGGTCTGGCTGGACGGCAT
AGAAAAACAGATGGCCGAACTCGGCATCTCCATGGCGGTCGCCCGTTATGAAATGCTCGGCCTGTTGAAGACCCTGATTG
AAGGCAGGGCCGGCAATGCAGCCTTTCCGTCGGCAACGTTGTCGCTCGCCGGCTTTATGGATGACAGGCTGAACCGCCCG
GCTGTCGATCTGGAAGACGAATATGGTCTCATGCTGCGGGATGGTCGGTATCGGGACGCAGCCGCAGGCCGCACGCTGGA
TGGGCCGCACCGGGTCGACCTTTTCGTGCGCCACGCGGAAAAGAACATGGAAGCCGAACGCTGTTCGACCGGGGAGCAGA
AGGCGCTGTTGGTGGGGCTGGTGCTTGCCCATGCGCAGCTGACAGCCAATATGACCGGTTACGCGCCAGTTCTGCTGCTG
GACGAAATTGCCGCTCATCTGGACGAGGGCAGGCGAGCCGCCCTGTTCGATCTCATCCACGCGCTGGGCGGTCAGAGTTT
CATGACCGGAACGGATGCGGCCATGTTCTCCGCTCTCGGCGAGCGGGCACAGTTCTTCAATGTTTCCCATGGAGGCATCA
CGGCATGA

Upstream 100 bases:

>100_bases
AGTTGCCACTACTGAATAATTCGCGTGGTTTTGGTGCTGGTCGATGGGGTCTGGCATTCGCCACCTTGCCATCCGGCCAG
CTTCGTTTTAGGCCGTAATG

Downstream 100 bases:

>100_bases
CGGCAGAGAAATGGAGGACGGCATGGACCCGCTGAGCCCGGAAGAAATCGAACGCTACAAGCGCCATATCCTGCTGCCGG
AAATCGGCGGTGCCGGGCAG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTNKVSLSRLKLTDFRNYAAAALVLDERHVVLTGDNGSGKTNLLEAVSFLSPGRGLRRAVLSDVTRVGAEATGFSIFADV
DGMDGEVAIGTGIEGDGEVVSRRLRLNGTPVKSVDELTDHLRVLWLTPAMDGLFTGSSSDRRRFLDRLVLSLDPGHGRRA
SDFEKAMRGRNRLLSEGRFDPVWLDGIEKQMAELGISMAVARYEMLGLLKTLIEGRAGNAAFPSATLSLAGFMDDRLNRP
AVDLEDEYGLMLRDGRYRDAAAGRTLDGPHRVDLFVRHAEKNMEAERCSTGEQKALLVGLVLAHAQLTANMTGYAPVLLL
DEIAAHLDEGRRAALFDLIHALGGQSFMTGTDAAMFSALGERAQFFNVSHGGITA

Sequences:

>Translated_375_residues
MTNKVSLSRLKLTDFRNYAAAALVLDERHVVLTGDNGSGKTNLLEAVSFLSPGRGLRRAVLSDVTRVGAEATGFSIFADV
DGMDGEVAIGTGIEGDGEVVSRRLRLNGTPVKSVDELTDHLRVLWLTPAMDGLFTGSSSDRRRFLDRLVLSLDPGHGRRA
SDFEKAMRGRNRLLSEGRFDPVWLDGIEKQMAELGISMAVARYEMLGLLKTLIEGRAGNAAFPSATLSLAGFMDDRLNRP
AVDLEDEYGLMLRDGRYRDAAAGRTLDGPHRVDLFVRHAEKNMEAERCSTGEQKALLVGLVLAHAQLTANMTGYAPVLLL
DEIAAHLDEGRRAALFDLIHALGGQSFMTGTDAAMFSALGERAQFFNVSHGGITA
>Mature_374_residues
TNKVSLSRLKLTDFRNYAAAALVLDERHVVLTGDNGSGKTNLLEAVSFLSPGRGLRRAVLSDVTRVGAEATGFSIFADVD
GMDGEVAIGTGIEGDGEVVSRRLRLNGTPVKSVDELTDHLRVLWLTPAMDGLFTGSSSDRRRFLDRLVLSLDPGHGRRAS
DFEKAMRGRNRLLSEGRFDPVWLDGIEKQMAELGISMAVARYEMLGLLKTLIEGRAGNAAFPSATLSLAGFMDDRLNRPA
VDLEDEYGLMLRDGRYRDAAAGRTLDGPHRVDLFVRHAEKNMEAERCSTGEQKALLVGLVLAHAQLTANMTGYAPVLLLD
EIAAHLDEGRRAALFDLIHALGGQSFMTGTDAAMFSALGERAQFFNVSHGGITA

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=373, Percent_Identity=28.686327077748, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_AGRT5 (Q8UJ65)

Other databases:

- EMBL:   AE007869
- PIR:   AD2585
- PIR:   C97367
- RefSeq:   NP_353107.2
- ProteinModelPortal:   Q8UJ65
- SMR:   Q8UJ65
- STRING:   Q8UJ65
- GeneID:   1132111
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0073
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q8UJ65
- ProtClustDB:   PRK00064
- BioCyc:   ATUM176299-1:ATU0073-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- GO:   GO:0009295
- HAMAP:   MF_00365
- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406
- Gene3D:   G3DSA:3.40.1140.10
- SMART:   SM00382
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40646; Mature: 40515

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKVSLSRLKLTDFRNYAAAALVLDERHVVLTGDNGSGKTNLLEAVSFLSPGRGLRRAV
CCCCCCHHHEEHHHHHHHHEEEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHH
LSDVTRVGAEATGFSIFADVDGMDGEVAIGTGIEGDGEVVSRRLRLNGTPVKSVDELTDH
HHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRVLWLTPAMDGLFTGSSSDRRRFLDRLVLSLDPGHGRRASDFEKAMRGRNRLLSEGRFD
HEEEEECCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PVWLDGIEKQMAELGISMAVARYEMLGLLKTLIEGRAGNAAFPSATLSLAGFMDDRLNRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
AVDLEDEYGLMLRDGRYRDAAAGRTLDGPHRVDLFVRHAEKNMEAERCSTGEQKALLVGL
CCCCCCCCCEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHHHHH
VLAHAQLTANMTGYAPVLLLDEIAAHLDEGRRAALFDLIHALGGQSFMTGTDAAMFSALG
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ERAQFFNVSHGGITA
HHHHEEECCCCCCCC
>Mature Secondary Structure 
TNKVSLSRLKLTDFRNYAAAALVLDERHVVLTGDNGSGKTNLLEAVSFLSPGRGLRRAV
CCCCCHHHEEHHHHHHHHEEEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHH
LSDVTRVGAEATGFSIFADVDGMDGEVAIGTGIEGDGEVVSRRLRLNGTPVKSVDELTDH
HHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRVLWLTPAMDGLFTGSSSDRRRFLDRLVLSLDPGHGRRASDFEKAMRGRNRLLSEGRFD
HEEEEECCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PVWLDGIEKQMAELGISMAVARYEMLGLLKTLIEGRAGNAAFPSATLSLAGFMDDRLNRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
AVDLEDEYGLMLRDGRYRDAAAGRTLDGPHRVDLFVRHAEKNMEAERCSTGEQKALLVGL
CCCCCCCCCEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHHHHH
VLAHAQLTANMTGYAPVLLLDEIAAHLDEGRRAALFDLIHALGGQSFMTGTDAAMFSALG
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ERAQFFNVSHGGITA
HHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194