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The map label for this gene is nrdF [H]

Identifier: 159184148

GI number: 159184148

Start: 73777

End: 74751

Strand: Direct

Name: nrdF [H]

Synonym: Atu0071

Alternate gene names: 159184148

Gene position: 73777-74751 (Clockwise)

Preceding gene: 159184147

Following gene: 159184149

Centisome position: 2.6

GC content: 56.62

Gene sequence:

>975_bases
ATGAACATCGCCGTAAAACCAGCGTCCCGCATCCGCGCGGTCAACTGGAACCGCATTGAGGATGACAAGGACCTCGAAGT
CTGGAACCGCCTGACCTCGAACTTCTGGCTGCCTGAAAAGGTCCCGCTTTCTAACGACATTCCCTCCTGGGGAACACTGA
AGCCGGAAGAACAGGAACTGACGATCCGTGTCTTCACCGGGCTGACATTGCTCGACACCATCCAGAACGGTGTAGGCGCC
GTGCGCCTGATGCCGGATTCGGCGACGCCACATGAGGAGGCGGTGCTCTCCAACATCTCGTTCATGGAGGCCGTGCACGC
CCGTTCTTATTCGTCGATCTTCTCGACGCTCTGCTCCACGCCTGACGTCGATGATGCCTATCGCTGGTCTGAGGAGAACG
AGTTCCTGCAACGCAAATCCGCGATCATCATGCGGGAATATGACAGCGGCGATCCGCTGAAGAAGAAGATCGCCAGCGTG
TTTCTGGAAAGCTTCCTGTTCTATTCCGGCTTCTACCTGCCGATGTTCTGGTCAAGCCGCGCCAAGCTCACCAACACGGC
AGACCTCATCCGCCTCATCATCCGCGATGAGGCCGTGCACGGCTATTATATCGGTTACAAGTTCCAGCGGGCGCTGGACC
GTCTTGGCGAGGCGCAAAGGCAGGAGATCAAGGATTTCGCCTTCGATCTCCTCCTCGAGCTTTACGACAATGAGGCGAAA
TATACCGAAGACCTTTATGACGGCGTCGGCCTAACGGAAGACGTCAAGCAGTTCCTGCATTACAACGCCAACAAGGCCCT
GATGAATCTGGGTTATGAAGCGCTGTTTCCCGCCGAAGCCTGCCGGGTCAATCCGGCGATCCTCTCGGCGCTCTCACCCA
ATGCCGATGAAAATCACGACTTTTTCTCAGGTTCCGGCTCGTCCTATGTCATTGGCAAGGCGGTCGCCACCGAGGATGAA
GACTGGGATTTCTGA

Upstream 100 bases:

>100_bases
ATCAAGACCATCTATTACATTCGGTTGCGCCAGATGGCGCTGGAAGGCACGCAGGTTCAGGGCTGCGTTTCCTGCACGCT
CTGATTTGAGGGACAAGAGA

Downstream 100 bases:

>100_bases
AGCCGGGACTGTCAGCACAAGTCCACAAACTATTGCGGGTTCACCACCTGACTCGCAAGCCGGAAGGGGCTGGAGACGGT
TGCGCCGATTGCATCGAACA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNIAVKPASRIRAVNWNRIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWGTLKPEEQELTIRVFTGLTLLDTIQNGVGA
VRLMPDSATPHEEAVLSNISFMEAVHARSYSSIFSTLCSTPDVDDAYRWSEENEFLQRKSAIIMREYDSGDPLKKKIASV
FLESFLFYSGFYLPMFWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQRALDRLGEAQRQEIKDFAFDLLLELYDNEAK
YTEDLYDGVGLTEDVKQFLHYNANKALMNLGYEALFPAEACRVNPAILSALSPNADENHDFFSGSGSSYVIGKAVATEDE
DWDF

Sequences:

>Translated_324_residues
MNIAVKPASRIRAVNWNRIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWGTLKPEEQELTIRVFTGLTLLDTIQNGVGA
VRLMPDSATPHEEAVLSNISFMEAVHARSYSSIFSTLCSTPDVDDAYRWSEENEFLQRKSAIIMREYDSGDPLKKKIASV
FLESFLFYSGFYLPMFWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQRALDRLGEAQRQEIKDFAFDLLLELYDNEAK
YTEDLYDGVGLTEDVKQFLHYNANKALMNLGYEALFPAEACRVNPAILSALSPNADENHDFFSGSGSSYVIGKAVATEDE
DWDF
>Mature_324_residues
MNIAVKPASRIRAVNWNRIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWGTLKPEEQELTIRVFTGLTLLDTIQNGVGA
VRLMPDSATPHEEAVLSNISFMEAVHARSYSSIFSTLCSTPDVDDAYRWSEENEFLQRKSAIIMREYDSGDPLKKKIASV
FLESFLFYSGFYLPMFWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQRALDRLGEAQRQEIKDFAFDLLLELYDNEAK
YTEDLYDGVGLTEDVKQFLHYNANKALMNLGYEALFPAEACRVNPAILSALSPNADENHDFFSGSGSSYVIGKAVATEDE
DWDF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI289177074, Length=259, Percent_Identity=23.5521235521236, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI42544136, Length=259, Percent_Identity=23.5521235521236, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789031, Length=316, Percent_Identity=77.8481012658228, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 36941; Mature: 36941

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAVKPASRIRAVNWNRIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWGTLKPEEQEL
CCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCE
TIRVFTGLTLLDTIQNGVGAVRLMPDSATPHEEAVLSNISFMEAVHARSYSSIFSTLCST
EEEEEHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDVDDAYRWSEENEFLQRKSAIIMREYDSGDPLKKKIASVFLESFLFYSGFYLPMFWSSR
CCCCHHHHCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
AKLTNTADLIRLIIRDEAVHGYYIGYKFQRALDRLGEAQRQEIKDFAFDLLLELYDNEAK
CCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YTEDLYDGVGLTEDVKQFLHYNANKALMNLGYEALFPAEACRVNPAILSALSPNADENHD
HHHHHHCCCCCHHHHHHHHHCCCCHHHHHCCHHHHCCHHHHHCCHHHHHHCCCCCCCCCC
FFSGSGSSYVIGKAVATEDEDWDF
CCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MNIAVKPASRIRAVNWNRIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWGTLKPEEQEL
CCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCE
TIRVFTGLTLLDTIQNGVGAVRLMPDSATPHEEAVLSNISFMEAVHARSYSSIFSTLCST
EEEEEHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDVDDAYRWSEENEFLQRKSAIIMREYDSGDPLKKKIASVFLESFLFYSGFYLPMFWSSR
CCCCHHHHCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
AKLTNTADLIRLIIRDEAVHGYYIGYKFQRALDRLGEAQRQEIKDFAFDLLLELYDNEAK
CCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YTEDLYDGVGLTEDVKQFLHYNANKALMNLGYEALFPAEACRVNPAILSALSPNADENHD
HHHHHHCCCCCHHHHHHHHHCCCCHHHHHCCHHHHCCHHHHHCCHHHHHHCCCCCCCCCC
FFSGSGSSYVIGKAVATEDEDWDF
CCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]