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The map label for this gene is moeB [H]

Identifier: 159184150

GI number: 159184150

Start: 77195

End: 77956

Strand: Direct

Name: moeB [H]

Synonym: Atu0074

Alternate gene names: 159184150

Gene position: 77195-77956 (Clockwise)

Preceding gene: 159184149

Following gene: 15887428

Centisome position: 2.72

GC content: 60.63

Gene sequence:

>762_bases
ATGGACCCGCTGAGCCCGGAAGAAATCGAACGCTACAAGCGCCATATCCTGCTGCCGGAAATCGGCGGTGCCGGGCAGCA
GCGGCTGAAAGCGGCGCGTGTGCTGGTGATAGGCGCGGGCGGTCTCGGCGCTCCCGTCCTTCATTATCTCGCCGCCGCCG
GGGTCGGCACGCTCGGCATGATCGATGATGATGTGGTCTCCCTCTCCAATCTCCAGCGGCAGGTGATCCACGACACCGGC
ACCATTGGCGAGTTGAAAACGGAAAGTGCCCGCAAGACCATCGCCCGGCTCAATCCGCATGTCCGCACGCTTATGTATGA
AGAACGTTTCTCGCAGGTCTGGGCGCACGACCACCTGCCATCCTTCGATCTCCTGATCGATGGTTCGGACAACTTCGATA
CACGTTACGCCGCAGCGGATGCCGCGGAGGCGGCAAAAAGACCGCTGGTCACAGGCGCTGTTGGTCGTTTCGATGGCTCG
GTAACGGTCCTGAAACCCTATGAGACCAACGCGGAAGGCAGTTTGCTGCCGGGTTATCGCGACCTGTTCCCGGCGCCGCC
GCCACAGGGCCTCATTCCCAACTGCGCGGAAACGGGCATTGTCGGCGCGCTGACCGGGGTCATCGGTACGCTGATGGCGA
TGGAAGCAATCAAGGTGATTACCGGTGCCGGTGAGCCCCTCATTGGAAGACTGCTGCTTTACGATGCTTTGTCGGCGCGG
TTCGAAACGGTAAAATACAAACGTCGGGCAAGGGAGAAATAA

Upstream 100 bases:

>100_bases
ACGGATGCGGCCATGTTCTCCGCTCTCGGCGAGCGGGCACAGTTCTTCAATGTTTCCCATGGAGGCATCACGGCATGACG
GCAGAGAAATGGAGGACGGC

Downstream 100 bases:

>100_bases
ACCATGATCGAGATAGCGAAGATCGGCGATGACTTCGATCGCTGGCAGGATCTGCTGGCGCTCATCATGACCTCCTTTGC
CTATATGGATGGTGTCATTG

Product: molybdopterin biosynthesis protein MoeB

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MDPLSPEEIERYKRHILLPEIGGAGQQRLKAARVLVIGAGGLGAPVLHYLAAAGVGTLGMIDDDVVSLSNLQRQVIHDTG
TIGELKTESARKTIARLNPHVRTLMYEERFSQVWAHDHLPSFDLLIDGSDNFDTRYAAADAAEAAKRPLVTGAVGRFDGS
VTVLKPYETNAEGSLLPGYRDLFPAPPPQGLIPNCAETGIVGALTGVIGTLMAMEAIKVITGAGEPLIGRLLLYDALSAR
FETVKYKRRAREK

Sequences:

>Translated_253_residues
MDPLSPEEIERYKRHILLPEIGGAGQQRLKAARVLVIGAGGLGAPVLHYLAAAGVGTLGMIDDDVVSLSNLQRQVIHDTG
TIGELKTESARKTIARLNPHVRTLMYEERFSQVWAHDHLPSFDLLIDGSDNFDTRYAAADAAEAAKRPLVTGAVGRFDGS
VTVLKPYETNAEGSLLPGYRDLFPAPPPQGLIPNCAETGIVGALTGVIGTLMAMEAIKVITGAGEPLIGRLLLYDALSAR
FETVKYKRRAREK
>Mature_253_residues
MDPLSPEEIERYKRHILLPEIGGAGQQRLKAARVLVIGAGGLGAPVLHYLAAAGVGTLGMIDDDVVSLSNLQRQVIHDTG
TIGELKTESARKTIARLNPHVRTLMYEERFSQVWAHDHLPSFDLLIDGSDNFDTRYAAADAAEAAKRPLVTGAVGRFDGS
VTVLKPYETNAEGSLLPGYRDLFPAPPPQGLIPNCAETGIVGALTGVIGTLMAMEAIKVITGAGEPLIGRLLLYDALSAR
FETVKYKRRAREK

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=238, Percent_Identity=45.3781512605042, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI23510338, Length=193, Percent_Identity=23.8341968911917, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI23510340, Length=193, Percent_Identity=23.8341968911917, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4885649, Length=134, Percent_Identity=28.3582089552239, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787048, Length=249, Percent_Identity=40.9638554216867, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI87082356, Length=246, Percent_Identity=37.8048780487805, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17540406, Length=246, Percent_Identity=35.3658536585366, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI193203301, Length=140, Percent_Identity=27.1428571428571, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321903, Length=254, Percent_Identity=39.7637795275591, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320598, Length=154, Percent_Identity=26.6233766233766, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582879, Length=246, Percent_Identity=41.0569105691057, Blast_Score=198, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24660640, Length=141, Percent_Identity=29.7872340425532, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28573937, Length=194, Percent_Identity=28.3505154639175, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 27251; Mature: 27251

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPLSPEEIERYKRHILLPEIGGAGQQRLKAARVLVIGAGGLGAPVLHYLAAAGVGTLGM
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCCCC
IDDDVVSLSNLQRQVIHDTGTIGELKTESARKTIARLNPHVRTLMYEERFSQVWAHDHLP
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCC
SFDLLIDGSDNFDTRYAAADAAEAAKRPLVTGAVGRFDGSVTVLKPYETNAEGSLLPGYR
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCHH
DLFPAPPPQGLIPNCAETGIVGALTGVIGTLMAMEAIKVITGAGEPLIGRLLLYDALSAR
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FETVKYKRRAREK
HHHHHHHHHHCCC
>Mature Secondary Structure
MDPLSPEEIERYKRHILLPEIGGAGQQRLKAARVLVIGAGGLGAPVLHYLAAAGVGTLGM
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCCCC
IDDDVVSLSNLQRQVIHDTGTIGELKTESARKTIARLNPHVRTLMYEERFSQVWAHDHLP
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCC
SFDLLIDGSDNFDTRYAAADAAEAAKRPLVTGAVGRFDGSVTVLKPYETNAEGSLLPGYR
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCHH
DLFPAPPPQGLIPNCAETGIVGALTGVIGTLMAMEAIKVITGAGEPLIGRLLLYDALSAR
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FETVKYKRRAREK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]