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The map label for this gene is hisB

Identifier: 159184137

GI number: 159184137

Start: 43805

End: 44401

Strand: Reverse

Name: hisB

Synonym: Atu0043

Alternate gene names: 159184137

Gene position: 44401-43805 (Counterclockwise)

Preceding gene: 159184139

Following gene: 15887400

Centisome position: 1.56

GC content: 58.63

Gene sequence:

>597_bases
ATGGCAGAACGTAAAGGCGAGATTATCCGCAAGACCAACGAAACCTCGGTTTCGGTGCGCGTGGATATCGACGGCACCGG
CAAATCGAAAATTTCCACCGGCGTGGGATTTTTCGACCATATGCTGGACCAGCTCAGCCGCCATTCGCTGATCGACATGG
ACATCGAAGTTCAGGGCGATCTGCATATCGATGACCACCACACCGTGGAAGACACCGGCATCGCCATCGGCCAGGCAATT
GCCAAGGCGCTGGGCGACCGGCGTGGCATTACCCGTTACGCCTCGCTCGATCTGGCCATGGACGAAACGATGACCAAGGC
TGCCGTCGATATTTCCGGCAGGCCGTTCCTTGTGTGGAACGTCAATTTCTCCGCGCCGAAGATCGGCACCTTCGATACCG
AACTGGTGCGCGAGTTCTTTCAGGCACTCGCCCAGAATGCCGGCATTACGCTGCATATCCTGAACCATTACGGCGCCAAC
AACCACCATATCGCCGAAACGTGCTTCAAGGCCGTTGCCCGCGTTCTTCGCACGGCAACCGAGATCGACCCCCGGCAGGC
AGGCCGCGTTCCCTCGACCAAGGGTATGCTGGCCTGA

Upstream 100 bases:

>100_bases
CGACGCACCGTTTTCGGCGCGTCACGCAAGACGATGGAACAACTGGATATTTGTGTCCCGTGCTGATAAGGAACGGCAAC
ATTTTCACGGAGCGGCCAAA

Downstream 100 bases:

>100_bases
CGCCAAAGCTGTCAGGAAGCCCATGACAAGCTATCTCGTTCTGGAAGCCCCCAACGGGCCGGACAGGGACCACAAGACGA
CCCGCTTCATTGCAGACCGT

Product: imidazoleglycerol-phosphate dehydratase

Products: NA

Alternate protein names: IGPD

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MAERKGEIIRKTNETSVSVRVDIDGTGKSKISTGVGFFDHMLDQLSRHSLIDMDIEVQGDLHIDDHHTVEDTGIAIGQAI
AKALGDRRGITRYASLDLAMDETMTKAAVDISGRPFLVWNVNFSAPKIGTFDTELVREFFQALAQNAGITLHILNHYGAN
NHHIAETCFKAVARVLRTATEIDPRQAGRVPSTKGMLA

Sequences:

>Translated_198_residues
MAERKGEIIRKTNETSVSVRVDIDGTGKSKISTGVGFFDHMLDQLSRHSLIDMDIEVQGDLHIDDHHTVEDTGIAIGQAI
AKALGDRRGITRYASLDLAMDETMTKAAVDISGRPFLVWNVNFSAPKIGTFDTELVREFFQALAQNAGITLHILNHYGAN
NHHIAETCFKAVARVLRTATEIDPRQAGRVPSTKGMLA
>Mature_197_residues
AERKGEIIRKTNETSVSVRVDIDGTGKSKISTGVGFFDHMLDQLSRHSLIDMDIEVQGDLHIDDHHTVEDTGIAIGQAIA
KALGDRRGITRYASLDLAMDETMTKAAVDISGRPFLVWNVNFSAPKIGTFDTELVREFFQALAQNAGITLHILNHYGANN
HHIAETCFKAVARVLRTATEIDPRQAGRVPSTKGMLA

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=195, Percent_Identity=48.2051282051282, Blast_Score=193, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6324776, Length=221, Percent_Identity=37.5565610859729, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_AGRT5 (Q8UJ89)

Other databases:

- EMBL:   AE007869
- PIR:   AD2582
- PIR:   B97364
- RefSeq:   NP_353082.2
- ProteinModelPortal:   Q8UJ89
- SMR:   Q8UJ89
- STRING:   Q8UJ89
- GeneID:   1132081
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0043
- eggNOG:   COG0131
- HOGENOM:   HBG289010
- OMA:   TLHVETL
- PhylomeDB:   Q8UJ89
- ProtClustDB:   PRK00951
- BioCyc:   ATUM176299-1:ATU0043-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00076
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR020568

Pfam domain/function: PF00475 IGPD; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =4.2.1.19

Molecular weight: Translated: 21725; Mature: 21594

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERKGEIIRKTNETSVSVRVDIDGTGKSKISTGVGFFDHMLDQLSRHSLIDMDIEVQGD
CCCCCCCHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCC
LHIDDHHTVEDTGIAIGQAIAKALGDRRGITRYASLDLAMDETMTKAAVDISGRPFLVWN
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEEE
VNFSAPKIGTFDTELVREFFQALAQNAGITLHILNHYGANNHHIAETCFKAVARVLRTAT
ECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
EIDPRQAGRVPSTKGMLA
HCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AERKGEIIRKTNETSVSVRVDIDGTGKSKISTGVGFFDHMLDQLSRHSLIDMDIEVQGD
CCCCCCHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCC
LHIDDHHTVEDTGIAIGQAIAKALGDRRGITRYASLDLAMDETMTKAAVDISGRPFLVWN
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEEE
VNFSAPKIGTFDTELVREFFQALAQNAGITLHILNHYGANNHHIAETCFKAVARVLRTAT
ECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
EIDPRQAGRVPSTKGMLA
HCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194