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The map label for this gene is cbiP

Identifier: 15890055

GI number: 15890055

Start: 2810940

End: 2812397

Strand: Direct

Name: cbiP

Synonym: Atu2808

Alternate gene names: 15890055

Gene position: 2810940-2812397 (Clockwise)

Preceding gene: 159185391

Following gene: 15890057

Centisome position: 98.92

GC content: 61.11

Gene sequence:

>1458_bases
ATGACAGCACGGGTGCTGATGTTTCAGGGAACCGGCTCCGATGTCGGCAAATCGCTGATGGTGGCCGGTCTTGCCCGGGC
ATTCACCCGGCGCGGCCTCTCCGTCGTGCCGTTCAAGCCACAGAACATGTCGAACAATGCCGCAGTTACCGCCGATGGCG
GTGAGATCGGCCGTGCGCAGGCGCTGCAGGCGCGGGCAGCAGGCGTGCCGCTTTCCGTGCACATGAACCCGGTGCTCCTG
AAGCCGCAGAGCGAAACCGGCGCACAGGTGGTAGTGCAGGGCAAGATCGTCGGCAGCGCCAAGGCGGCCGATTATCAGCA
CATGAAGGCCGGGCTGATGCCGCGCGTGCTTGATAGTTTCGAGCACTTGAAACAACTGGCCGATCTGGTGCTGGTGGAAG
GGGCAGGCAGCGCCTCGGAAATAAACCTCCGCGCCAATGACATCGCCAATATGGGTTTTGCCCGAGCGGCCGATGCGCCA
GTGATCCTGATCGGCGATATCGATCGCGGTGGCGTCATCGCCAGCCTCGTCGGCACCAGAGAGGTGCTCGACGACGATGA
CGCGGCGATGATCGAAGGTTTCATCGTCAACCGATTTCGCGGCGATCCGGCACTGTTTTCCGATGGCATGCGGATGATTG
CGGAAAAGACCGATTGGTCCTCCATAGGGCTTCTGCCGCATTTTTCCGATGCGGCGAAACTGCCGGCGGAGGATGCTCTG
GGGCTTTCCGGCCCAGCCCAACGAAAGCCAAGCGCAAAAATCCGCATCGCCGTGCCGATCCTGCCGCACATTTCCAATTT
CGACGATCTCGATCCCCTCGACATGGAGCCGGATGTGGAACTGATCCGGGTCCGTCCAGGTGAGACGATCCCCGCCGATT
GCCGTCTGGTTTTGCTGTGCGGCTCGAAATCGACCATCGCCGATCTCGCCGTGCTGAAAGATGCCGGCCTGGACATTGAT
ATAAAGGCGCATGTGAGACGCGGCGGCTACGTTCTCGGCCTTTGCGGCGGTTACCAGATGCTTGGAAAAACCGTTGCCGA
TCCTGACGGCATCGAGGGACCGCCGGCGACTGTCGAGGGGCTCGGCCTGCTGGACGTCGACACGGTTTTGACGGGCGACA
AACGGCTCGTTTCCGTTCGGGGCACGAGTTTCGACGGCGTTGGTTTATCCGGCTACGAAATGCATGTCGGTGAAACCACG
GGAATAGCGGGTAGTCTGGTGTTTTCGACGATCGACGGACATGCCGATGGCACCATTTCGCCCGACGGCCGCGTCTTCGG
CACCTATATTCACGGTCTTTTTGCGGATGACCGCCAGCGCAGCGCATGGCTCGAGCGTCTCGGCGGGCAGGGCACGGACC
TGAACTATGACGCGCAGGTTGATGCGGTTCTCGACCGTCTGGCTGCGCATATGGAACAGCACCTCGATCTCGACAGGCTG
CTTGCTATAGCGCGATAA

Upstream 100 bases:

>100_bases
CAGCCGCTGATCGACGCCGCCGATATGGTGACGGAAATGACGCTGGTGAAACATCACTTCAAGGCGGGCGTGAAGGCCCA
AGCCGGGATAGAATTCTGAC

Downstream 100 bases:

>100_bases
GGATCGCCAGAAGTCCGAACAGGCCAATCAGCAACAAGTCCGCGATCCGCGCCAGTCGCAATGCCTTGCGAATATCACCG
GCAACCAGTGCGGACCTCCC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTARVLMFQGTGSDVGKSLMVAGLARAFTRRGLSVVPFKPQNMSNNAAVTADGGEIGRAQALQARAAGVPLSVHMNPVLL
KPQSETGAQVVVQGKIVGSAKAADYQHMKAGLMPRVLDSFEHLKQLADLVLVEGAGSASEINLRANDIANMGFARAADAP
VILIGDIDRGGVIASLVGTREVLDDDDAAMIEGFIVNRFRGDPALFSDGMRMIAEKTDWSSIGLLPHFSDAAKLPAEDAL
GLSGPAQRKPSAKIRIAVPILPHISNFDDLDPLDMEPDVELIRVRPGETIPADCRLVLLCGSKSTIADLAVLKDAGLDID
IKAHVRRGGYVLGLCGGYQMLGKTVADPDGIEGPPATVEGLGLLDVDTVLTGDKRLVSVRGTSFDGVGLSGYEMHVGETT
GIAGSLVFSTIDGHADGTISPDGRVFGTYIHGLFADDRQRSAWLERLGGQGTDLNYDAQVDAVLDRLAAHMEQHLDLDRL
LAIAR

Sequences:

>Translated_485_residues
MTARVLMFQGTGSDVGKSLMVAGLARAFTRRGLSVVPFKPQNMSNNAAVTADGGEIGRAQALQARAAGVPLSVHMNPVLL
KPQSETGAQVVVQGKIVGSAKAADYQHMKAGLMPRVLDSFEHLKQLADLVLVEGAGSASEINLRANDIANMGFARAADAP
VILIGDIDRGGVIASLVGTREVLDDDDAAMIEGFIVNRFRGDPALFSDGMRMIAEKTDWSSIGLLPHFSDAAKLPAEDAL
GLSGPAQRKPSAKIRIAVPILPHISNFDDLDPLDMEPDVELIRVRPGETIPADCRLVLLCGSKSTIADLAVLKDAGLDID
IKAHVRRGGYVLGLCGGYQMLGKTVADPDGIEGPPATVEGLGLLDVDTVLTGDKRLVSVRGTSFDGVGLSGYEMHVGETT
GIAGSLVFSTIDGHADGTISPDGRVFGTYIHGLFADDRQRSAWLERLGGQGTDLNYDAQVDAVLDRLAAHMEQHLDLDRL
LAIAR
>Mature_484_residues
TARVLMFQGTGSDVGKSLMVAGLARAFTRRGLSVVPFKPQNMSNNAAVTADGGEIGRAQALQARAAGVPLSVHMNPVLLK
PQSETGAQVVVQGKIVGSAKAADYQHMKAGLMPRVLDSFEHLKQLADLVLVEGAGSASEINLRANDIANMGFARAADAPV
ILIGDIDRGGVIASLVGTREVLDDDDAAMIEGFIVNRFRGDPALFSDGMRMIAEKTDWSSIGLLPHFSDAAKLPAEDALG
LSGPAQRKPSAKIRIAVPILPHISNFDDLDPLDMEPDVELIRVRPGETIPADCRLVLLCGSKSTIADLAVLKDAGLDIDI
KAHVRRGGYVLGLCGGYQMLGKTVADPDGIEGPPATVEGLGLLDVDTVLTGDKRLVSVRGTSFDGVGLSGYEMHVGETTG
IAGSLVFSTIDGHADGTISPDGRVFGTYIHGLFADDRQRSAWLERLGGQGTDLNYDAQVDAVLDRLAAHMEQHLDLDRLL
AIAR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_AGRT5 (Q8UBP3)

Other databases:

- EMBL:   AE007869
- PIR:   AG2921
- PIR:   H97695
- RefSeq:   NP_355736.1
- ProteinModelPortal:   Q8UBP3
- STRING:   Q8UBP3
- GeneID:   1134846
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2808
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- ProtClustDB:   PRK00784
- BioCyc:   ATUM176299-1:ATU2808-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 51207; Mature: 51076

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 335-335 ACT_SITE 431-431

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARVLMFQGTGSDVGKSLMVAGLARAFTRRGLSVVPFKPQNMSNNAAVTADGGEIGRAQ
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCHHH
ALQARAAGVPLSVHMNPVLLKPQSETGAQVVVQGKIVGSAKAADYQHMKAGLMPRVLDSF
HHHHHHCCCEEEEECCEEEECCCCCCCCEEEEEEEEECCCCCCCHHHHHHCCHHHHHHHH
EHLKQLADLVLVEGAGSASEINLRANDIANMGFARAADAPVILIGDIDRGGVIASLVGTR
HHHHHHHHEEEEECCCCCEEEEEEHHHHHCCCCCCCCCCCEEEEECCCCCCEEEEHHCCH
EVLDDDDAAMIEGFIVNRFRGDPALFSDGMRMIAEKTDWSSIGLLPHFSDAAKLPAEDAL
HHCCCCCHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCHHCC
GLSGPAQRKPSAKIRIAVPILPHISNFDDLDPLDMEPDVELIRVRPGETIPADCRLVLLC
CCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEE
GSKSTIADLAVLKDAGLDIDIKAHVRRGGYVLGLCGGYQMLGKTVADPDGIEGPPATVEG
CCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
LGLLDVDTVLTGDKRLVSVRGTSFDGVGLSGYEMHVGETTGIAGSLVFSTIDGHADGTIS
CEEEEEHHEECCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCC
PDGRVFGTYIHGLFADDRQRSAWLERLGGQGTDLNYDAQVDAVLDRLAAHMEQHLDLDRL
CCCCEEEEEHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHH
LAIAR
HHHCC
>Mature Secondary Structure 
TARVLMFQGTGSDVGKSLMVAGLARAFTRRGLSVVPFKPQNMSNNAAVTADGGEIGRAQ
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCHHH
ALQARAAGVPLSVHMNPVLLKPQSETGAQVVVQGKIVGSAKAADYQHMKAGLMPRVLDSF
HHHHHHCCCEEEEECCEEEECCCCCCCCEEEEEEEEECCCCCCCHHHHHHCCHHHHHHHH
EHLKQLADLVLVEGAGSASEINLRANDIANMGFARAADAPVILIGDIDRGGVIASLVGTR
HHHHHHHHEEEEECCCCCEEEEEEHHHHHCCCCCCCCCCCEEEEECCCCCCEEEEHHCCH
EVLDDDDAAMIEGFIVNRFRGDPALFSDGMRMIAEKTDWSSIGLLPHFSDAAKLPAEDAL
HHCCCCCHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCHHCC
GLSGPAQRKPSAKIRIAVPILPHISNFDDLDPLDMEPDVELIRVRPGETIPADCRLVLLC
CCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEE
GSKSTIADLAVLKDAGLDIDIKAHVRRGGYVLGLCGGYQMLGKTVADPDGIEGPPATVEG
CCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
LGLLDVDTVLTGDKRLVSVRGTSFDGVGLSGYEMHVGETTGIAGSLVFSTIDGHADGTIS
CEEEEEHHEECCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCC
PDGRVFGTYIHGLFADDRQRSAWLERLGGQGTDLNYDAQVDAVLDRLAAHMEQHLDLDRL
CCCCEEEEEHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHH
LAIAR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194