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The map label for this gene is hemC

Identifier: 15889908

GI number: 15889908

Start: 2635681

End: 2636610

Strand: Direct

Name: hemC

Synonym: Atu2652

Alternate gene names: 15889908

Gene position: 2635681-2636610 (Clockwise)

Preceding gene: 15889902

Following gene: 15889909

Centisome position: 92.75

GC content: 61.94

Gene sequence:

>930_bases
ATGCAAACAAAACCTTTCCGAATCGGCACACGCGGCAGCCCGCTGGCGCTCGCGCAGGCTTATGAGACCCGCAGCCGGCT
GATGGCGGCACACGGTCTTCCGGAAGAGATGTTCGAAATCGTCGTGCTCTCCACCAAGGGCGACCGGATCACCGACCGCG
CCTTGTCGGAAATCGGCGGCAAGGGCCTGTTCACGGAGGAACTGGAAAACCAGCTCCTGTCCGGCGAACTCGATATTGCC
GTGCATTCCTCCAAGGACATGCCGACGGTTTTGCCTGAGGGCTTGCATCTTTCAGCTTTCCTGCCGCGTGAAGACATGCG
CGACGCCTTCATTGGTCGCACGGCACCGAAGCTTCTGGAGCTGCCGCAGGGCGCCGTTGTCGGCTCCGCCTCTCTGCGCC
GCCAGGCACTGATCCGCCGCCTGCGCCCGGATCTCAACGTCATCGTTTTCCGCGGTCTGGTCGACACGCGTCTGCGCAAG
CTCGAAGAAGGCCAGGCGGATGCGACGCTTCTTGCCTTTGCCGGCCTGAAGCGGCTCGGCAAGGACAACGTGCCGACGGA
AATTCTCGATCCAAAAGAATTCCCGCCCGCTCCGGCCCAAGGCGCGATCGGGGTCGAGAGCCGCATCGGTGACGCCCGCA
TGGATAAATTGCTGGCTCCCATAAACGACAGGCCCACTTATGACGCCGTGACCTGTGAGCGTGCCTTTCTGGCAGCGCTT
GACGGTTCCTGTCGTACCCCCATTGCCGGTTACGCCACCTGCGAAGGTGATAACCTGCATTTTTCCGGCCTGATCCTTAC
CCCCGATGGCCAGACAAGCCACGGCGTCGAAATCTCCGGCAACCGCAGGGACGCCTTGATACTGGGGAAAAAGGCCGGCG
AAGAGGTGCGCGCCAAAGCGGGCAGCAATTTCTTCGAAGGCTGGAGCTGA

Upstream 100 bases:

>100_bases
GCCGAGAATACGAAGAAAGGGGGGCATCAAATCTGTTCGTTCATTGCGATCATGCGCTGAGTTGGTCTAAGACAACTCCG
GTAACAATGGATAGACGCGG

Downstream 100 bases:

>100_bases
GCCGGTGCGGATCGTCGTCACCCGGCCGCAGCGTTCGGGCGAAAGGACGGCTGCAAAGCTTGAAGCGCTTGGCCATGCGC
CGGTGCTTCTGCCGCTGTTT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MQTKPFRIGTRGSPLALAQAYETRSRLMAAHGLPEEMFEIVVLSTKGDRITDRALSEIGGKGLFTEELENQLLSGELDIA
VHSSKDMPTVLPEGLHLSAFLPREDMRDAFIGRTAPKLLELPQGAVVGSASLRRQALIRRLRPDLNVIVFRGLVDTRLRK
LEEGQADATLLAFAGLKRLGKDNVPTEILDPKEFPPAPAQGAIGVESRIGDARMDKLLAPINDRPTYDAVTCERAFLAAL
DGSCRTPIAGYATCEGDNLHFSGLILTPDGQTSHGVEISGNRRDALILGKKAGEEVRAKAGSNFFEGWS

Sequences:

>Translated_309_residues
MQTKPFRIGTRGSPLALAQAYETRSRLMAAHGLPEEMFEIVVLSTKGDRITDRALSEIGGKGLFTEELENQLLSGELDIA
VHSSKDMPTVLPEGLHLSAFLPREDMRDAFIGRTAPKLLELPQGAVVGSASLRRQALIRRLRPDLNVIVFRGLVDTRLRK
LEEGQADATLLAFAGLKRLGKDNVPTEILDPKEFPPAPAQGAIGVESRIGDARMDKLLAPINDRPTYDAVTCERAFLAAL
DGSCRTPIAGYATCEGDNLHFSGLILTPDGQTSHGVEISGNRRDALILGKKAGEEVRAKAGSNFFEGWS
>Mature_309_residues
MQTKPFRIGTRGSPLALAQAYETRSRLMAAHGLPEEMFEIVVLSTKGDRITDRALSEIGGKGLFTEELENQLLSGELDIA
VHSSKDMPTVLPEGLHLSAFLPREDMRDAFIGRTAPKLLELPQGAVVGSASLRRQALIRRLRPDLNVIVFRGLVDTRLRK
LEEGQADATLLAFAGLKRLGKDNVPTEILDPKEFPPAPAQGAIGVESRIGDARMDKLLAPINDRPTYDAVTCERAFLAAL
DGSCRTPIAGYATCEGDNLHFSGLILTPDGQTSHGVEISGNRRDALILGKKAGEEVRAKAGSNFFEGWS

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=279, Percent_Identity=42.2939068100358, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI66933009, Length=275, Percent_Identity=42.9090909090909, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI48994974, Length=296, Percent_Identity=44.2567567567568, Blast_Score=221, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6319996, Length=257, Percent_Identity=37.3540856031128, Blast_Score=145, Evalue=6e-36,
Organism=Drosophila melanogaster, GI20130425, Length=264, Percent_Identity=40.1515151515151, Blast_Score=178, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_AGRT5 (Q8UC46)

Other databases:

- EMBL:   AE007869
- PIR:   AC2902
- PIR:   E97677
- RefSeq:   NP_355589.1
- ProteinModelPortal:   Q8UC46
- SMR:   Q8UC46
- STRING:   Q8UC46
- GeneID:   1134690
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2652
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- PhylomeDB:   Q8UC46
- ProtClustDB:   PRK00072
- BioCyc:   ATUM176299-1:ATU2652-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 33495; Mature: 33495

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKPFRIGTRGSPLALAQAYETRSRLMAAHGLPEEMFEIVVLSTKGDRITDRALSEIGG
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCCCHHHHHHHHHHCC
KGLFTEELENQLLSGELDIAVHSSKDMPTVLPEGLHLSAFLPREDMRDAFIGRTAPKLLE
CCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCHHHHH
LPQGAVVGSASLRRQALIRRLRPDLNVIVFRGLVDTRLRKLEEGQADATLLAFAGLKRLG
CCCCCEECHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
KDNVPTEILDPKEFPPAPAQGAIGVESRIGDARMDKLLAPINDRPTYDAVTCERAFLAAL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DGSCRTPIAGYATCEGDNLHFSGLILTPDGQTSHGVEISGNRRDALILGKKAGEEVRAKA
CCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEECCCCHHHHHHC
GSNFFEGWS
CCCCCCCCC
>Mature Secondary Structure
MQTKPFRIGTRGSPLALAQAYETRSRLMAAHGLPEEMFEIVVLSTKGDRITDRALSEIGG
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCCCHHHHHHHHHHCC
KGLFTEELENQLLSGELDIAVHSSKDMPTVLPEGLHLSAFLPREDMRDAFIGRTAPKLLE
CCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCHHHHH
LPQGAVVGSASLRRQALIRRLRPDLNVIVFRGLVDTRLRKLEEGQADATLLAFAGLKRLG
CCCCCEECHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
KDNVPTEILDPKEFPPAPAQGAIGVESRIGDARMDKLLAPINDRPTYDAVTCERAFLAAL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DGSCRTPIAGYATCEGDNLHFSGLILTPDGQTSHGVEISGNRRDALILGKKAGEEVRAKA
CCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEECCCCHHHHHHC
GSNFFEGWS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194