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The map label for this gene is actI [H]

Identifier: 15889796

GI number: 15889796

Start: 2504459

End: 2504998

Strand: Reverse

Name: actI [H]

Synonym: Atu2531

Alternate gene names: 15889796

Gene position: 2504998-2504459 (Counterclockwise)

Preceding gene: 159185257

Following gene: 159185254

Centisome position: 88.16

GC content: 61.48

Gene sequence:

>540_bases
ATGACATCAGCATTGTTTGGCCTGAGCGGCCTAGCGCCTGAAGGCGTCGGCCAGAATTTCCGTTCGACGATGCGCCGTTT
TCCGGCAACCGTGACGGTCATTACCGCCTGCGCCGCCGGTGATCAGCGCGACCATGGCATGACGGTGACAGCCGTCACCT
CGGTTTCGATGGAGCCGCCGTCTCTGCTGGTCTGCCTGAATAATCGTACCCTGCTGCATGAATTGCTGCTGTGCCGGCCC
GATTTCATCGTCAACGTGCTGACGCAGGATCAGATCGCGCTTTCGGATGGCTTCAGCGGCAAGCTGCCGCCGGAGGAGCG
TTTCCGTGACGGCAGTTGGCAACGCCACGATAATGGCGTTCTTTACCTGCCGACGGCCCATGCCGCCATCGCCTGCCGCC
GGGTTGCGGCCATGCCCTATGGAACCCATACCGTCTTCATCGGACAGGTGGTTTCGGCCGATGTCAGCGAGACCACACGG
CCGCTGCTTTACGAAAATGCCCAATATTGCGCCGCAAGCCCCGCGCACGAACCCGTCTGA

Upstream 100 bases:

>100_bases
GGAATGACGGGGTTCCCAGTGGTATAAAATGACGAAAAACATTTGCATTGACAAATGTTGCAAATGAAAATAAAAAGATT
TCAGGATTTGGGAGGATCTC

Downstream 100 bases:

>100_bases
ATTTCAGCGCTCAGATCATTCCCGCAAGGAGACAATCATGAACGACATGAGCCATGCGCCGCTGCCGGTGCAAACGAACC
CGCATGTCCGCCTCGCCAGC

Product: actinorhodin polyketide dimerase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MTSALFGLSGLAPEGVGQNFRSTMRRFPATVTVITACAAGDQRDHGMTVTAVTSVSMEPPSLLVCLNNRTLLHELLLCRP
DFIVNVLTQDQIALSDGFSGKLPPEERFRDGSWQRHDNGVLYLPTAHAAIACRRVAAMPYGTHTVFIGQVVSADVSETTR
PLLYENAQYCAASPAHEPV

Sequences:

>Translated_179_residues
MTSALFGLSGLAPEGVGQNFRSTMRRFPATVTVITACAAGDQRDHGMTVTAVTSVSMEPPSLLVCLNNRTLLHELLLCRP
DFIVNVLTQDQIALSDGFSGKLPPEERFRDGSWQRHDNGVLYLPTAHAAIACRRVAAMPYGTHTVFIGQVVSADVSETTR
PLLYENAQYCAASPAHEPV
>Mature_178_residues
TSALFGLSGLAPEGVGQNFRSTMRRFPATVTVITACAAGDQRDHGMTVTAVTSVSMEPPSLLVCLNNRTLLHELLLCRPD
FIVNVLTQDQIALSDGFSGKLPPEERFRDGSWQRHDNGVLYLPTAHAAIACRRVAAMPYGTHTVFIGQVVSADVSETTRP
LLYENAQYCAASPAHEPV

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=152, Percent_Identity=32.2368421052632, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 19370; Mature: 19239

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSALFGLSGLAPEGVGQNFRSTMRRFPATVTVITACAAGDQRDHGMTVTAVTSVSMEPP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEEEEEEECCCCC
SLLVCLNNRTLLHELLLCRPDFIVNVLTQDQIALSDGFSGKLPPEERFRDGSWQRHDNGV
EEEEEECCCHHHHHHHHCCCCHHEEECCCCCEEECCCCCCCCCCHHHHCCCCCEECCCCE
LYLPTAHAAIACRRVAAMPYGTHTVFIGQVVSADVSETTRPLLYENAQYCAASPAHEPV
EEECCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCHHHHHCCHHHCCCCHHCCCCCCCCC
>Mature Secondary Structure 
TSALFGLSGLAPEGVGQNFRSTMRRFPATVTVITACAAGDQRDHGMTVTAVTSVSMEPP
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEEEEEEECCCCC
SLLVCLNNRTLLHELLLCRPDFIVNVLTQDQIALSDGFSGKLPPEERFRDGSWQRHDNGV
EEEEEECCCHHHHHHHHCCCCHHEEECCCCCEEECCCCCCCCCCHHHHCCCCCEECCCCE
LYLPTAHAAIACRRVAAMPYGTHTVFIGQVVSADVSETTRPLLYENAQYCAASPAHEPV
EEECCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCHHHHHCCHHHCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA