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The map label for this gene is hsaA [H]

Identifier: 159185254

GI number: 159185254

Start: 2503192

End: 2504421

Strand: Reverse

Name: hsaA [H]

Synonym: Atu2530

Alternate gene names: 159185254

Gene position: 2504421-2503192 (Counterclockwise)

Preceding gene: 15889796

Following gene: 15889794

Centisome position: 88.13

GC content: 64.63

Gene sequence:

>1230_bases
ATGAACGACATGAGCCATGCGCCGCTGCCGGTGCAAACGAACCCGCATGTCCGCCTCGCCAGCCGCGTTGCCGGCATAGC
CGATCTCTTCCGCTCCAGCGCCAGGCAGACGGAGGAGGCGCGGCGCGTACCTGCCAGCCATATAGAGGCGCTGCGTGGCA
TCGGTTATTTCGACATCGTCAAGCCCCGCGCCTTCAACGGCAAGGGTGGCGATTTTGCCGAGCTGGTGGAAGCCAATATC
GAGCTTTCGGCGGCCTGTGCGTCGACGGGCTGGGTCGCCGGCCTGCTCTCTGCCCATCAATGGCTGCTGGCGATGTTTCC
AGAAGAGGCGCAGGCCGATGTCTGGGGTGAAAATCCCGATGCGCTGCTGTGCGGTTCCTATGCGCCGGTGAAGATGGCGG
AGGCTGCCGATGGCGGTTATCGCCTCAGCGGCAAATGGGCCTTTGCTTCTGGCTGCGAGAACGCACAATGGTCTCTCTGC
GCCGCCATTCTGCCGCCGAAGGGTGAAGGGAAGCCGGTTCCCGCCTTTCTGCTCGTTCCCGCCAGCCAATATGTCGTTGA
AGACACCTGGCATGTTGTGGGGCTGGCCGGCACGGGATCGAAGACGCTGGTTCTCGATGACGTCTTCGTTCCCGAACACC
GGGTCTTGACCTTTCCGGACGCCACCAGCGGCTGCACGCCCGGTGGACGATATTATGCGGAAGAGAGCCTGTTCAACATG
CCGCTTCTGACCGGCATTCCCTCCTGTCTTGCGGCGGCCGGTGTCGGCGCGGCCAAGGGGGCGCTTGCCGCCTATGTCGA
TCATGTCGGCGGTCGGGTCACGCGCGGCGCGGTGGCGGGTGGCAATAACCGCATGGCGGAATTCCCGACCATCCAGCTGC
GCGTCGCGGAAGCGGCGGCAAGTGTCGATGCGGCCTGCGAAATCCTGCTGCGCGACGTCTCGCGCGCTCAGGCGCTGGCA
CAGGCCCGGATCGAAGGCCGCGCGGAATTTACCGTGGATGATCGCCTTCTGAGCCGTCGCGGCCAGTCCTTTGCCGTGTC
CCTCTCATTGCGTGCCGTGCAGGCGCTGAATGATTCCACCGGTGGCGTCGGGCTCGATCTGGCGAACCCCGTGCAGCGCG
CCTGGCGGGATGCCAATGCGGTCGGCCGTCACATCAGCATGAACTGGGATGCCGTCGGCACCATGATTGGCCAGAGCATG
CTCGGCCTCGAACCCAAGGGCCAATACTGA

Upstream 100 bases:

>100_bases
CGGCCGCTGCTTTACGAAAATGCCCAATATTGCGCCGCAAGCCCCGCGCACGAACCCGTCTGAATTTCAGCGCTCAGATC
ATTCCCGCAAGGAGACAATC

Downstream 100 bases:

>100_bases
TCGCCACTGAGGCGGCAAACCAACAGCGAAATTTGGAGGAAACTACATGCAAGGCAAGGTCGCTCTCGAGGAGCATTTCG
CAATCCCGGAAACACTTCAA

Product: oxidoreductase

Products: NA

Alternate protein names: 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione 4-hydroxylase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MNDMSHAPLPVQTNPHVRLASRVAGIADLFRSSARQTEEARRVPASHIEALRGIGYFDIVKPRAFNGKGGDFAELVEANI
ELSAACASTGWVAGLLSAHQWLLAMFPEEAQADVWGENPDALLCGSYAPVKMAEAADGGYRLSGKWAFASGCENAQWSLC
AAILPPKGEGKPVPAFLLVPASQYVVEDTWHVVGLAGTGSKTLVLDDVFVPEHRVLTFPDATSGCTPGGRYYAEESLFNM
PLLTGIPSCLAAAGVGAAKGALAAYVDHVGGRVTRGAVAGGNNRMAEFPTIQLRVAEAAASVDAACEILLRDVSRAQALA
QARIEGRAEFTVDDRLLSRRGQSFAVSLSLRAVQALNDSTGGVGLDLANPVQRAWRDANAVGRHISMNWDAVGTMIGQSM
LGLEPKGQY

Sequences:

>Translated_409_residues
MNDMSHAPLPVQTNPHVRLASRVAGIADLFRSSARQTEEARRVPASHIEALRGIGYFDIVKPRAFNGKGGDFAELVEANI
ELSAACASTGWVAGLLSAHQWLLAMFPEEAQADVWGENPDALLCGSYAPVKMAEAADGGYRLSGKWAFASGCENAQWSLC
AAILPPKGEGKPVPAFLLVPASQYVVEDTWHVVGLAGTGSKTLVLDDVFVPEHRVLTFPDATSGCTPGGRYYAEESLFNM
PLLTGIPSCLAAAGVGAAKGALAAYVDHVGGRVTRGAVAGGNNRMAEFPTIQLRVAEAAASVDAACEILLRDVSRAQALA
QARIEGRAEFTVDDRLLSRRGQSFAVSLSLRAVQALNDSTGGVGLDLANPVQRAWRDANAVGRHISMNWDAVGTMIGQSM
LGLEPKGQY
>Mature_409_residues
MNDMSHAPLPVQTNPHVRLASRVAGIADLFRSSARQTEEARRVPASHIEALRGIGYFDIVKPRAFNGKGGDFAELVEANI
ELSAACASTGWVAGLLSAHQWLLAMFPEEAQADVWGENPDALLCGSYAPVKMAEAADGGYRLSGKWAFASGCENAQWSLC
AAILPPKGEGKPVPAFLLVPASQYVVEDTWHVVGLAGTGSKTLVLDDVFVPEHRVLTFPDATSGCTPGGRYYAEESLFNM
PLLTGIPSCLAAAGVGAAKGALAAYVDHVGGRVTRGAVAGGNNRMAEFPTIQLRVAEAAASVDAACEILLRDVSRAQALA
QARIEGRAEFTVDDRLLSRRGQSFAVSLSLRAVQALNDSTGGVGLDLANPVQRAWRDANAVGRHISMNWDAVGTMIGQSM
LGLEPKGQY

Specific function: Catalyzes the o-hydroxylation of 3-hydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy- 9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Also catalyzes the o-hydroxylation of a range of

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013107
- InterPro:   IPR006092
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF08028 Acyl-CoA_dh_2; PF02771 Acyl-CoA_dh_N [H]

EC number: NA

Molecular weight: Translated: 43210; Mature: 43210

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMSHAPLPVQTNPHVRLASRVAGIADLFRSSARQTEEARRVPASHIEALRGIGYFDIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEE
KPRAFNGKGGDFAELVEANIELSAACASTGWVAGLLSAHQWLLAMFPEEAQADVWGENPD
CCCCCCCCCCCHHHHHHCCCEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCC
ALLCGSYAPVKMAEAADGGYRLSGKWAFASGCENAQWSLCAAILPPKGEGKPVPAFLLVP
EEEECCCCCEEEHHCCCCCEEEECEEEECCCCCCCCEEEEEEECCCCCCCCCCCEEEEEC
ASQYVVEDTWHVVGLAGTGSKTLVLDDVFVPEHRVLTFPDATSGCTPGGRYYAEESLFNM
CCHHEEECCEEEEEEECCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHCC
PLLTGIPSCLAAAGVGAAKGALAAYVDHVGGRVTRGAVAGGNNRMAEFPTIQLRVAEAAA
CHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEECEEECCCCCEEECCEEEEEEHHHHH
SVDAACEILLRDVSRAQALAQARIEGRAEFTVDDRLLSRRGQSFAVSLSLRAVQALNDST
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCEEEEEEEHHHHHHHCCCC
GGVGLDLANPVQRAWRDANAVGRHISMNWDAVGTMIGQSMLGLEPKGQY
CCCCCCCCHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDMSHAPLPVQTNPHVRLASRVAGIADLFRSSARQTEEARRVPASHIEALRGIGYFDIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEE
KPRAFNGKGGDFAELVEANIELSAACASTGWVAGLLSAHQWLLAMFPEEAQADVWGENPD
CCCCCCCCCCCHHHHHHCCCEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCC
ALLCGSYAPVKMAEAADGGYRLSGKWAFASGCENAQWSLCAAILPPKGEGKPVPAFLLVP
EEEECCCCCEEEHHCCCCCEEEECEEEECCCCCCCCEEEEEEECCCCCCCCCCCEEEEEC
ASQYVVEDTWHVVGLAGTGSKTLVLDDVFVPEHRVLTFPDATSGCTPGGRYYAEESLFNM
CCHHEEECCEEEEEEECCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHCC
PLLTGIPSCLAAAGVGAAKGALAAYVDHVGGRVTRGAVAGGNNRMAEFPTIQLRVAEAAA
CHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEECEEECCCCCEEECCEEEEEEHHHHH
SVDAACEILLRDVSRAQALAQARIEGRAEFTVDDRLLSRRGQSFAVSLSLRAVQALNDST
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCEEEEEEEHHHHHHHCCCC
GGVGLDLANPVQRAWRDANAVGRHISMNWDAVGTMIGQSMLGLEPKGQY
CCCCCCCCHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]