Click here to switch to the map view.

The map label for this gene is hemF

Identifier: 15889523

GI number: 15889523

Start: 2219983

End: 2220894

Strand: Direct

Name: hemF

Synonym: Atu2247

Alternate gene names: 15889523

Gene position: 2219983-2220894 (Clockwise)

Preceding gene: 159185120

Following gene: 159185122

Centisome position: 78.12

GC content: 61.18

Gene sequence:

>912_bases
ATGGAACGGCCAATCTTGCCGAAGGGCTTGCCCGAGGACATTGAAGACAAGAAGGCCGTTGCCCAGGCGTGGTTCCAGCA
TCTGCGCGACACCATCGTCGCCTCCTTCGAAACCCTAGAAGACGAACTCACTGGCCCGCTTTCCGATCAGGAGCCTGGTC
GCTTCGTGCAGAAGGACTGGCTGCGCGACAATGGCGAGGGCGGCGGCGGCAAGATGTCGATGATGGAAGGTCGCGTCTTC
GAAAAGGTCGGCGTGCACACCTCCACCGTCTATGGCGAATTCTCGCCCGAGTTCCGCAAGCAGATACCCGGTGCGGAAGA
AGACCCGCGCTTCTGGGCCTCCGGCCTTTCGCTTATCGCCCATCCGGTCAATCCCAATGTGCCGGCCGTGCACATGAACA
CCCGCATGGTCGTCACCACCAGCCATTGGTTCGGCGGCGGTGCGGATCTGACGCCGGTGCTCGGCCGCAGGCGGACACAG
CAGGACCCGGATACCCAGCTGTTCCACCGCGCCTTCGAGATTACCTGCAACCGTCATCCGATCGCCGATTATCCGCGATA
CAAGAGCTGGTGCGACGAGTATTTCTTCCTGAAGCACCGCGACGAGCCGCGTGGCACCGGCGGCATCTTTTTCGACTGGC
TGCACCCTGACGAGGAGAAAGGTGGCTGGGACGCCAATTTCACCTTCGTGCAGGATGTCGGCCGCGCCTTCAACCTCGTC
TATCCGAAAATCGTCCGCGCCAATTTCAACCAGAACTGGACGGAAGAGGACCGCGACGAGCAGCTCATCCGCCGCGGCCG
CTACGTGGAATTCAACCTGCTTTACGATCGCGGCACGATCTTCGGTCTGAAGACCGGAGGTAACGTCGAATCAATCCTGT
CATCGCTGCCGCCGGTGGTGCGCTGGCCTTAA

Upstream 100 bases:

>100_bases
ATAGAGATAGTGTCCGCATGTGAACTTGGCGAGTCGAATTTGTGCATGAAGCCGGCACCTGCTAGACGCGACAGGGAATC
GACTTCTAAGGAGACTTTGC

Downstream 100 bases:

>100_bases
AAAACCTCACGAAAATTTGACGCTTATCAGCCATTTGGTCGCAAATAGCGCAAATTAGGACTTATGGAAAAATCAAGGCA
GTGGTAGTCTCCTCCCGCAG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MERPILPKGLPEDIEDKKAVAQAWFQHLRDTIVASFETLEDELTGPLSDQEPGRFVQKDWLRDNGEGGGGKMSMMEGRVF
EKVGVHTSTVYGEFSPEFRKQIPGAEEDPRFWASGLSLIAHPVNPNVPAVHMNTRMVVTTSHWFGGGADLTPVLGRRRTQ
QDPDTQLFHRAFEITCNRHPIADYPRYKSWCDEYFFLKHRDEPRGTGGIFFDWLHPDEEKGGWDANFTFVQDVGRAFNLV
YPKIVRANFNQNWTEEDRDEQLIRRGRYVEFNLLYDRGTIFGLKTGGNVESILSSLPPVVRWP

Sequences:

>Translated_303_residues
MERPILPKGLPEDIEDKKAVAQAWFQHLRDTIVASFETLEDELTGPLSDQEPGRFVQKDWLRDNGEGGGGKMSMMEGRVF
EKVGVHTSTVYGEFSPEFRKQIPGAEEDPRFWASGLSLIAHPVNPNVPAVHMNTRMVVTTSHWFGGGADLTPVLGRRRTQ
QDPDTQLFHRAFEITCNRHPIADYPRYKSWCDEYFFLKHRDEPRGTGGIFFDWLHPDEEKGGWDANFTFVQDVGRAFNLV
YPKIVRANFNQNWTEEDRDEQLIRRGRYVEFNLLYDRGTIFGLKTGGNVESILSSLPPVVRWP
>Mature_303_residues
MERPILPKGLPEDIEDKKAVAQAWFQHLRDTIVASFETLEDELTGPLSDQEPGRFVQKDWLRDNGEGGGGKMSMMEGRVF
EKVGVHTSTVYGEFSPEFRKQIPGAEEDPRFWASGLSLIAHPVNPNVPAVHMNTRMVVTTSHWFGGGADLTPVLGRRRTQ
QDPDTQLFHRAFEITCNRHPIADYPRYKSWCDEYFFLKHRDEPRGTGGIFFDWLHPDEEKGGWDANFTFVQDVGRAFNLV
YPKIVRANFNQNWTEEDRDEQLIRRGRYVEFNLLYDRGTIFGLKTGGNVESILSSLPPVVRWP

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=263, Percent_Identity=42.5855513307985, Blast_Score=183, Evalue=2e-46,
Organism=Escherichia coli, GI1788777, Length=276, Percent_Identity=43.8405797101449, Blast_Score=202, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320249, Length=299, Percent_Identity=40.8026755852843, Blast_Score=202, Evalue=7e-53,
Organism=Drosophila melanogaster, GI17864388, Length=308, Percent_Identity=39.6103896103896, Blast_Score=194, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_AGRT5 (Q8UD80)

Other databases:

- EMBL:   AE007869
- PIR:   AF2852
- PIR:   D97629
- RefSeq:   NP_355204.1
- ProteinModelPortal:   Q8UD80
- SMR:   Q8UD80
- STRING:   Q8UD80
- GeneID:   1134285
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2247
- eggNOG:   COG0408
- HOGENOM:   HBG631180
- OMA:   HMNTRFV
- PhylomeDB:   Q8UD80
- ProtClustDB:   PRK05330
- BioCyc:   ATUM176299-1:ATU2247-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 34800; Mature: 34800

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 131-131 BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERPILPKGLPEDIEDKKAVAQAWFQHLRDTIVASFETLEDELTGPLSDQEPGRFVQKDW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LRDNGEGGGGKMSMMEGRVFEKVGVHTSTVYGEFSPEFRKQIPGAEEDPRFWASGLSLIA
HCCCCCCCCCEEHHHHCHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHCHHHEE
HPVNPNVPAVHMNTRMVVTTSHWFGGGADLTPVLGRRRTQQDPDTQLFHRAFEITCNRHP
ECCCCCCCEEEECCEEEEEECCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
IADYPRYKSWCDEYFFLKHRDEPRGTGGIFFDWLHPDEEKGGWDANFTFVQDVGRAFNLV
CCCCCHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
YPKIVRANFNQNWTEEDRDEQLIRRGRYVEFNLLYDRGTIFGLKTGGNVESILSSLPPVV
HHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCEEEEEECCCCHHHHHHHCCCCC
RWP
CCC
>Mature Secondary Structure
MERPILPKGLPEDIEDKKAVAQAWFQHLRDTIVASFETLEDELTGPLSDQEPGRFVQKDW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LRDNGEGGGGKMSMMEGRVFEKVGVHTSTVYGEFSPEFRKQIPGAEEDPRFWASGLSLIA
HCCCCCCCCCEEHHHHCHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHCHHHEE
HPVNPNVPAVHMNTRMVVTTSHWFGGGADLTPVLGRRRTQQDPDTQLFHRAFEITCNRHP
ECCCCCCCEEEECCEEEEEECCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
IADYPRYKSWCDEYFFLKHRDEPRGTGGIFFDWLHPDEEKGGWDANFTFVQDVGRAFNLV
CCCCCHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
YPKIVRANFNQNWTEEDRDEQLIRRGRYVEFNLLYDRGTIFGLKTGGNVESILSSLPPVV
HHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCEEEEEECCCCHHHHHHHCCCCC
RWP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194