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The map label for this gene is alaS [H]

Identifier: 15888552

GI number: 15888552

Start: 1210203

End: 1210940

Strand: Reverse

Name: alaS [H]

Synonym: Atu1216

Alternate gene names: 15888552

Gene position: 1210940-1210203 (Counterclockwise)

Preceding gene: 15888553

Following gene: 15888551

Centisome position: 42.62

GC content: 58.81

Gene sequence:

>738_bases
ATGCCTGTGAATGCCCTGTTCCGTGACGATTTTTATCTTTCTACCGCAGAAGCGATCGTCACGGCGGTGCATGAGGATGG
CGGCATCGAACTGGACCAGACCTGCTTTTACGCCACATCCGGCGGCCAGCCCGGCGATAGCGGCTTTCTCGAAAGGGCTG
ACGGATCTCGCATCGAGCTCGGCGTCACGAAAAATGGTGCCGACAAGAGCGTGATCGTCCATGTGCCACTCGAAGGCCAG
CCTTCCCCCGAGATCGGCGAGAAGCTGACCCTGCATGTGGACTGGCCGCGTCGCTACAAGCTGATGCGCATGCACACTGC
CTGCCATTTACTCTCTGTTGTCTGCCAGTGGCCGATCACCGGTGCGGCGGTCGGCGAGGACGAATCGCGTGTCGATTTCG
ACATGTCGGAGACCATCGAAAAGGATGAAGTGACGGCAAAGCTGATGGAGCTGGTGAAAGCCAACCATCCCGTCTTCCTG
CAATGGATCAGCGACGAGGAGTTGCAGGCCAATCCGGGCATCGTCAAATCCAAGAATGTGCGCCCGCCAATGGGCCTCGG
CCGGGTCAGCCTCGTCTGCATCGGCGAGAATTCCAGCATCGACAGCCAGCCCTGCGGCGGAACCCATGTTTCCGAAACGC
AGGAAGTGGGCGATATTCACATTGCCAAGATAGAAAAGAAGGGCAAGGAGAACAGGCGGTTCCGCATTCGTTTCGGCGCG
CCTGAAGCTGCTGCCTGA

Upstream 100 bases:

>100_bases
TCCGGATTTCCTGCGCTCCAAGGGGCTTCCCGTGCCGCAGTGGCTGGCGACCAAGAGCGAGATTCCGGTTCCCTACGAAA
CCGTTTGAGGACATGCCGAA

Downstream 100 bases:

>100_bases
AATCAGGAGAGACATCGTGAGCACGGATAAGAGCCGTTTCGTCGTTTCGGCGGACTGGGTGGAAAAACAACTCGGCACGG
CCGGGTTTCGTGTCGTTGAT

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPVNALFRDDFYLSTAEAIVTAVHEDGGIELDQTCFYATSGGQPGDSGFLERADGSRIELGVTKNGADKSVIVHVPLEGQ
PSPEIGEKLTLHVDWPRRYKLMRMHTACHLLSVVCQWPITGAAVGEDESRVDFDMSETIEKDEVTAKLMELVKANHPVFL
QWISDEELQANPGIVKSKNVRPPMGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGDIHIAKIEKKGKENRRFRIRFGA
PEAAA

Sequences:

>Translated_245_residues
MPVNALFRDDFYLSTAEAIVTAVHEDGGIELDQTCFYATSGGQPGDSGFLERADGSRIELGVTKNGADKSVIVHVPLEGQ
PSPEIGEKLTLHVDWPRRYKLMRMHTACHLLSVVCQWPITGAAVGEDESRVDFDMSETIEKDEVTAKLMELVKANHPVFL
QWISDEELQANPGIVKSKNVRPPMGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGDIHIAKIEKKGKENRRFRIRFGA
PEAAA
>Mature_244_residues
PVNALFRDDFYLSTAEAIVTAVHEDGGIELDQTCFYATSGGQPGDSGFLERADGSRIELGVTKNGADKSVIVHVPLEGQP
SPEIGEKLTLHVDWPRRYKLMRMHTACHLLSVVCQWPITGAAVGEDESRVDFDMSETIEKDEVTAKLMELVKANHPVFLQ
WISDEELQANPGIVKSKNVRPPMGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGDIHIAKIEKKGKENRRFRIRFGAP
EAAA

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=240, Percent_Identity=29.5833333333333, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1789048, Length=231, Percent_Identity=27.7056277056277, Blast_Score=67, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24582809, Length=243, Percent_Identity=30.8641975308642, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45552267, Length=243, Percent_Identity=30.8641975308642, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 26838; Mature: 26706

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVNALFRDDFYLSTAEAIVTAVHEDGGIELDQTCFYATSGGQPGDSGFLERADGSRIEL
CCCCCEECCCCCHHHHHHHHEEEECCCCCEECCEEEEEECCCCCCCCCCEECCCCCEEEE
GVTKNGADKSVIVHVPLEGQPSPEIGEKLTLHVDWPRRYKLMRMHTACHLLSVVCQWPIT
EEECCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
GAAVGEDESRVDFDMSETIEKDEVTAKLMELVKANHPVFLQWISDEELQANPGIVKSKNV
CEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCC
RPPMGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGDIHIAKIEKKGKENRRFRIRFGA
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCEEEEEECC
PEAAA
CCCCC
>Mature Secondary Structure 
PVNALFRDDFYLSTAEAIVTAVHEDGGIELDQTCFYATSGGQPGDSGFLERADGSRIEL
CCCCEECCCCCHHHHHHHHEEEECCCCCEECCEEEEEECCCCCCCCCCEECCCCCEEEE
GVTKNGADKSVIVHVPLEGQPSPEIGEKLTLHVDWPRRYKLMRMHTACHLLSVVCQWPIT
EEECCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
GAAVGEDESRVDFDMSETIEKDEVTAKLMELVKANHPVFLQWISDEELQANPGIVKSKNV
CEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCC
RPPMGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGDIHIAKIEKKGKENRRFRIRFGA
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCEEEEEECC
PEAAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]