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The map label for this gene is sseA [H]

Identifier: 15888551

GI number: 15888551

Start: 1208765

End: 1210186

Strand: Reverse

Name: sseA [H]

Synonym: Atu1215

Alternate gene names: 15888551

Gene position: 1210186-1208765 (Counterclockwise)

Preceding gene: 15888552

Following gene: 15888548

Centisome position: 42.59

GC content: 62.59

Gene sequence:

>1422_bases
GTGAGCACGGATAAGAGCCGTTTCGTCGTTTCGGCGGACTGGGTGGAAAAACAACTCGGCACGGCCGGGTTTCGTGTCGT
TGATGCGTCCTGGTATCTTCCGGCGCACAAGCGCAACGGCGCGGAGGAATATGCCGCCGGCCACCTGCCGGGTGCCGTGT
TCTTCGACCAGGACAAGATCGCCGATCACACGACCGGCCTGCCGCATTCCCTGCCCTCGCCGGAATTCTTCGCGCAAGAG
GCCGGCGAACTTGGTCTTAGCGAGAATGATACGATCGTGGTCTATGACGGCCCCGGTTTCTTCTCCGCGCCGCGTGTGTG
GTGGATGCTGCGGGTGATGGGCGTGCGCAAGGCCTATGTGCTGGATGGCGGCCTCGACGGCTGGAAGCGCGAAGGCCGGC
CGCTTGAAACCGGCACACCGGAGATCGAACCCGCCACCTTCACACCCGATTTCAACGACAAGCGCGTGACCTCGCTATCG
ACCATGCGCGGCATCGTCGATAGCGGTGAAAAGCAGATTGCCGATGCCCGGGGTGCCGGACGTTTCACCGGCGACGAGGC
CGAGCCGCGTGCCGGCATGCGCTCCGGCCACATGCCCGGCGCGCGCAGCCTGCCGGCGATGGCCTTTTCCGACAACGGTC
ACTTCAAGGATCTGACCGCCATACGCAAGATGGTCACCGATGCCGGCATCGATCTTTCCAAGCCAGTGGTGACAAGCTGT
GGCTCGGGCGTCACCGCCGCCGTCGTTACGCTGGCACTGGAATCGCTCGGTCATCACGATAACTCGCTCTATGACGGCTC
GTGGTCGGAATGGGGTGGGCTCGAAGATACGCCCGTCGTCACCGGCGCTGCCGATCCCCTGCCCGTCATTTCCCATGGCC
CGCTGAAGGCGCATGTGACACAGCTGGAAATGACCGCACCGCCAAAGGTCAGCCTGCCGATCCCGGTCAACCTCCAGACG
GCGCTGATGCGGGTCAATAATATCCCCCTGCACTTCTACCGCTATCTCTACTGGCGGGTGGGGAAACGCTGGCATTGGCA
GAAGCGCATGCGCATGAGCGATGCCGAGCTTTCCGCCATCCTGCGCGATCCGAAAAACAGCGTCACGGTTCTCTATATGA
ACGGCGCGCCCGCCGGTTTCTTCGAACTGAACAAGGCGAGCGACGAAGTGACCGAGCTTTCCTATTTCGGGCTGCTCGAA
GAGGCGATCGGCGCGGGCGTCGGCAAATGGTTCCTTCTGCAGGCGCTTTACGCCGCCTGGCAGGACAACCCGCAGCGGGT
GACGGTGACCACGAATACGCTCGACCATCCGCGCGCATTGCAGCTTTACCAGATGATGGGCTTCTCGCCCGTCGGCACCT
ATGAGGCCTGGGTGGAACCGCTTGCCGATACGGAGCTTCTGGAAATTTCGCTGCGTAACTGA

Upstream 100 bases:

>100_bases
ATTCACATTGCCAAGATAGAAAAGAAGGGCAAGGAGAACAGGCGGTTCCGCATTCGTTTCGGCGCGCCTGAAGCTGCTGC
CTGAAATCAGGAGAGACATC

Downstream 100 bases:

>100_bases
GGGTGTGGCTCTCCCAGCCTGCCGCATATACCGCTCTCAATTAGGGTGCGCCCGACCCCACTCCCTCATTCCTGTGCTCG
TCACAGTAATCCAGTCAGCC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSTDKSRFVVSADWVEKQLGTAGFRVVDASWYLPAHKRNGAEEYAAGHLPGAVFFDQDKIADHTTGLPHSLPSPEFFAQE
AGELGLSENDTIVVYDGPGFFSAPRVWWMLRVMGVRKAYVLDGGLDGWKREGRPLETGTPEIEPATFTPDFNDKRVTSLS
TMRGIVDSGEKQIADARGAGRFTGDEAEPRAGMRSGHMPGARSLPAMAFSDNGHFKDLTAIRKMVTDAGIDLSKPVVTSC
GSGVTAAVVTLALESLGHHDNSLYDGSWSEWGGLEDTPVVTGAADPLPVISHGPLKAHVTQLEMTAPPKVSLPIPVNLQT
ALMRVNNIPLHFYRYLYWRVGKRWHWQKRMRMSDAELSAILRDPKNSVTVLYMNGAPAGFFELNKASDEVTELSYFGLLE
EAIGAGVGKWFLLQALYAAWQDNPQRVTVTTNTLDHPRALQLYQMMGFSPVGTYEAWVEPLADTELLEISLRN

Sequences:

>Translated_473_residues
MSTDKSRFVVSADWVEKQLGTAGFRVVDASWYLPAHKRNGAEEYAAGHLPGAVFFDQDKIADHTTGLPHSLPSPEFFAQE
AGELGLSENDTIVVYDGPGFFSAPRVWWMLRVMGVRKAYVLDGGLDGWKREGRPLETGTPEIEPATFTPDFNDKRVTSLS
TMRGIVDSGEKQIADARGAGRFTGDEAEPRAGMRSGHMPGARSLPAMAFSDNGHFKDLTAIRKMVTDAGIDLSKPVVTSC
GSGVTAAVVTLALESLGHHDNSLYDGSWSEWGGLEDTPVVTGAADPLPVISHGPLKAHVTQLEMTAPPKVSLPIPVNLQT
ALMRVNNIPLHFYRYLYWRVGKRWHWQKRMRMSDAELSAILRDPKNSVTVLYMNGAPAGFFELNKASDEVTELSYFGLLE
EAIGAGVGKWFLLQALYAAWQDNPQRVTVTTNTLDHPRALQLYQMMGFSPVGTYEAWVEPLADTELLEISLRN
>Mature_472_residues
STDKSRFVVSADWVEKQLGTAGFRVVDASWYLPAHKRNGAEEYAAGHLPGAVFFDQDKIADHTTGLPHSLPSPEFFAQEA
GELGLSENDTIVVYDGPGFFSAPRVWWMLRVMGVRKAYVLDGGLDGWKREGRPLETGTPEIEPATFTPDFNDKRVTSLST
MRGIVDSGEKQIADARGAGRFTGDEAEPRAGMRSGHMPGARSLPAMAFSDNGHFKDLTAIRKMVTDAGIDLSKPVVTSCG
SGVTAAVVTLALESLGHHDNSLYDGSWSEWGGLEDTPVVTGAADPLPVISHGPLKAHVTQLEMTAPPKVSLPIPVNLQTA
LMRVNNIPLHFYRYLYWRVGKRWHWQKRMRMSDAELSAILRDPKNSVTVLYMNGAPAGFFELNKASDEVTELSYFGLLEE
AIGAGVGKWFLLQALYAAWQDNPQRVTVTTNTLDHPRALQLYQMMGFSPVGTYEAWVEPLADTELLEISLRN

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI61835204, Length=304, Percent_Identity=40.1315789473684, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI194473681, Length=304, Percent_Identity=40.1315789473684, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI194473668, Length=304, Percent_Identity=40.1315789473684, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=40.2930402930403, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI87082121, Length=276, Percent_Identity=42.7536231884058, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87081967, Length=278, Percent_Identity=26.6187050359712, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71997283, Length=250, Percent_Identity=28, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17561888, Length=295, Percent_Identity=24.7457627118644, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17559150, Length=279, Percent_Identity=26.5232974910394, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI115534702, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543836, Length=274, Percent_Identity=26.2773722627737, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543838, Length=274, Percent_Identity=26.2773722627737, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324825, Length=270, Percent_Identity=35.5555555555556, Blast_Score=156, Evalue=5e-39,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 52009; Mature: 51878

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDKSRFVVSADWVEKQLGTAGFRVVDASWYLPAHKRNGAEEYAAGHLPGAVFFDQDKI
CCCCCCEEEEEHHHHHHHHCCCCEEEEECEEECCCCCCCCHHHHHCCCCCCEEEECCCHH
ADHTTGLPHSLPSPEFFAQEAGELGLSENDTIVVYDGPGFFSAPRVWWMLRVMGVRKAYV
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEE
LDGGLDGWKREGRPLETGTPEIEPATFTPDFNDKRVTSLSTMRGIVDSGEKQIADARGAG
EECCCCCHHHCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCC
RFTGDEAEPRAGMRSGHMPGARSLPAMAFSDNGHFKDLTAIRKMVTDAGIDLSKPVVTSC
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHC
GSGVTAAVVTLALESLGHHDNSLYDGSWSEWGGLEDTPVVTGAADPLPVISHGPLKAHVT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCEEECCCCCCCCCCCCCCEEEEE
QLEMTAPPKVSLPIPVNLQTALMRVNNIPLHFYRYLYWRVGKRWHWQKRMRMSDAELSAI
EEEECCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHH
LRDPKNSVTVLYMNGAPAGFFELNKASDEVTELSYFGLLEEAIGAGVGKWFLLQALYAAW
HHCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QDNPQRVTVTTNTLDHPRALQLYQMMGFSPVGTYEAWVEPLADTELLEISLRN
CCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure 
STDKSRFVVSADWVEKQLGTAGFRVVDASWYLPAHKRNGAEEYAAGHLPGAVFFDQDKI
CCCCCEEEEEHHHHHHHHCCCCEEEEECEEECCCCCCCCHHHHHCCCCCCEEEECCCHH
ADHTTGLPHSLPSPEFFAQEAGELGLSENDTIVVYDGPGFFSAPRVWWMLRVMGVRKAYV
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEE
LDGGLDGWKREGRPLETGTPEIEPATFTPDFNDKRVTSLSTMRGIVDSGEKQIADARGAG
EECCCCCHHHCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCC
RFTGDEAEPRAGMRSGHMPGARSLPAMAFSDNGHFKDLTAIRKMVTDAGIDLSKPVVTSC
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHC
GSGVTAAVVTLALESLGHHDNSLYDGSWSEWGGLEDTPVVTGAADPLPVISHGPLKAHVT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCEEECCCCCCCCCCCCCCEEEEE
QLEMTAPPKVSLPIPVNLQTALMRVNNIPLHFYRYLYWRVGKRWHWQKRMRMSDAELSAI
EEEECCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHH
LRDPKNSVTVLYMNGAPAGFFELNKASDEVTELSYFGLLEEAIGAGVGKWFLLQALYAAW
HHCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QDNPQRVTVTTNTLDHPRALQLYQMMGFSPVGTYEAWVEPLADTELLEISLRN
CCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]