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The map label for this gene is hisF

Identifier: 15887397

GI number: 15887397

Start: 41118

End: 41894

Strand: Reverse

Name: hisF

Synonym: Atu0039

Alternate gene names: 15887397

Gene position: 41894-41118 (Counterclockwise)

Preceding gene: 15887398

Following gene: 159184135

Centisome position: 1.47

GC content: 58.94

Gene sequence:

>777_bases
ATGACCCTCAAAGCCCGCATTATTCCCTGCCTCGACGTGAAAGACGGACGTGTCGTCAAGGGTGTGAACTTCGTCGACCT
GATCGATGCTGGCGACCCGGTCGAGGCAGCAAAAGCCTATGACGCGGCGGGAGCGGATGAACTCTGCTTCCTCGACATCA
CCGCGTCCTCGGACAATCGCGACACCATCTTCGATGTCGTCTCACGCACTGCGGACCATTGCTTCATGCCGGTGACGGTC
GGTGGCGGGGTTCGTAGCGTCGCGGATATTCGTAAGCTGCTTCTTTGCGGTGCGGACAAGGTCTCTATCAACTCCGCAGC
TGTCAAGGATCCCGACTTCGTTGCGCAGGCGGCCGACAAGTTCGGCAACCAATGCATCGTCGTTTCCATCGACGCCAAGC
GGGTTTCCAAAGATGGCGAAGCTGACCGCTGGGAAATTTTTACCCATGGCGGACGCCAGCCAACTGGTATCGATGCCGTG
GAATTCGCCATCAAGATGGTTGAACGGGGTGCCGGCGAATTGCTCGTCACCTCGATGGATCGCGACGGCACCAAGAGCGG
TTATGATATCGGCCTGACGCGCAGCATTGCCGATCAGGTTCGCGTGCCCGTCATCGCCTCGGGCGGCGTTGGTACGCTTG
ATGATCTGGTGGCAGGTGTTCGCGATGGCCACGCGACGGCAGTGCTTGCCGCTTCCATCTTCCACTTCGGCACCTATTCG
ATCGGTGAAGCCAAGAGCTACATGGCCGAACATGGCATTGCCATGCGTCTCGACTGA

Upstream 100 bases:

>100_bases
CAAAGCTCGAGGGTGCGATTTCAGGCCGTGCCCTTTATGACGGGCGTATCGACCCCACCGAAGCGCTTGACCTCATCAAG
GCCGCGAAGGAGGTACGTGC

Downstream 100 bases:

>100_bases
TTGCAGGAACATCCCATGAGCGCATTTTCCCTTTCCGATCTGGAACGCATCGTCGCGAAGCGTGCCGCTGCATCCCCTGA
TGAATCCTGGACGGCCAAGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTLKARIIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNRDTIFDVVSRTADHCFMPVTV
GGGVRSVADIRKLLLCGADKVSINSAAVKDPDFVAQAADKFGNQCIVVSIDAKRVSKDGEADRWEIFTHGGRQPTGIDAV
EFAIKMVERGAGELLVTSMDRDGTKSGYDIGLTRSIADQVRVPVIASGGVGTLDDLVAGVRDGHATAVLAASIFHFGTYS
IGEAKSYMAEHGIAMRLD

Sequences:

>Translated_258_residues
MTLKARIIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNRDTIFDVVSRTADHCFMPVTV
GGGVRSVADIRKLLLCGADKVSINSAAVKDPDFVAQAADKFGNQCIVVSIDAKRVSKDGEADRWEIFTHGGRQPTGIDAV
EFAIKMVERGAGELLVTSMDRDGTKSGYDIGLTRSIADQVRVPVIASGGVGTLDDLVAGVRDGHATAVLAASIFHFGTYS
IGEAKSYMAEHGIAMRLD
>Mature_257_residues
TLKARIIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNRDTIFDVVSRTADHCFMPVTVG
GGVRSVADIRKLLLCGADKVSINSAAVKDPDFVAQAADKFGNQCIVVSIDAKRVSKDGEADRWEIFTHGGRQPTGIDAVE
FAIKMVERGAGELLVTSMDRDGTKSGYDIGLTRSIADQVRVPVIASGGVGTLDDLVAGVRDGHATAVLAASIFHFGTYSI
GEAKSYMAEHGIAMRLD

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=42.8571428571429, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=30.327868852459, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=30.7936507936508, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_AGRT5 (P58799)

Other databases:

- EMBL:   AE007869
- PIR:   AH2581
- PIR:   F97363
- RefSeq:   NP_353078.1
- ProteinModelPortal:   P58799
- SMR:   P58799
- STRING:   P58799
- GeneID:   1132077
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0039
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   P58799
- ProtClustDB:   PRK02083
- BioCyc:   ATUM176299-1:ATU0039-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27266; Mature: 27135

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKARIIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNR
CCCCEEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCC
DTIFDVVSRTADHCFMPVTVGGGVRSVADIRKLLLCGADKVSINSAAVKDPDFVAQAADK
HHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHH
FGNQCIVVSIDAKRVSKDGEADRWEIFTHGGRQPTGIDAVEFAIKMVERGAGELLVTSMD
HCCEEEEEEECHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECC
RDGTKSGYDIGLTRSIADQVRVPVIASGGVGTLDDLVAGVRDGHATAVLAASIFHFGTYS
CCCCCCCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC
IGEAKSYMAEHGIAMRLD
CCHHHHHHHHCCEEEEEC
>Mature Secondary Structure 
TLKARIIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNR
CCCEEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCC
DTIFDVVSRTADHCFMPVTVGGGVRSVADIRKLLLCGADKVSINSAAVKDPDFVAQAADK
HHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHH
FGNQCIVVSIDAKRVSKDGEADRWEIFTHGGRQPTGIDAVEFAIKMVERGAGELLVTSMD
HCCEEEEEEECHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECC
RDGTKSGYDIGLTRSIADQVRVPVIASGGVGTLDDLVAGVRDGHATAVLAASIFHFGTYS
CCCCCCCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC
IGEAKSYMAEHGIAMRLD
CCHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194