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The map label for this gene is pyk [H]

Identifier: 239617997

GI number: 239617997

Start: 1743675

End: 1745093

Strand: Direct

Name: pyk [H]

Synonym: Kole_1625

Alternate gene names: 239617997

Gene position: 1743675-1745093 (Clockwise)

Preceding gene: 239617996

Following gene: 239617998

Centisome position: 75.74

GC content: 42.85

Gene sequence:

>1419_bases
ATGAGGAAGACGAAAATAGTTTGTACTGTTGGGCCGGCAACAGAAAATCCCGATATGTTAAAAAAACTCATAGAAGCTGG
CATGAATGTGGTGAGATTAAACACTTCGCATGATGATTTAGAACATCATCGTCGGAGAATTAGAACAGTTAAGAAAATTC
GTGAAGAACTTGCTGTGCCTATCACTATATTACTTGACCTTGCCGGCCCAAAGATAAGGACGGGAGACTTTTCTTCAGAT
ACGGTTGTTCTTAAGAAAGGAGACCTATTCACTCTCACGACAGAGGATATTGTTGGTGATGATAAGCGAGTGAGTGTAAG
TTACAAAAAACTACCATCTGAAGTTAATTCTGGAGACTTCATCCTCGTAAACGACGGAAAAATAAAGTTGAAGGTTGTAG
AAACGAACGAGACAGAGATAAAAACCACCGTCGTTAACGGTGGGGAAATAACGCACCGTAGAGGAATAAACGTTCCTGGA
ATCGATCTTGGAATAGAAGCTCTTACTTTAAAGGATAAGGAATTTATAAAGTTGGGCATCGAAGAAGGCGTAGACTACTT
TGCACTGTCTTTTGTCAGGAAACCCGAGGATGTTATCGAAGCTAAGAAGATCATTTCAAGCTTTGGTGGCAGCATTCCGA
TAATTTCGAAAATAGAAACAGTGCAGGCTCTAAAAAGAATAGAGTCTATCGCTGAGGTCTCAGATGGTCTTATGGTAGCT
CGGGGTGATCTTGGTGTCGAAATTCCTGTGGAAGAAGTTCCAATAGCTCAAAAAAAGATAATACGCGCAGGAAATAAAAA
TAGAATCCCGGTAATAACAGCTACTCAAATGCTTGAATCAATGATAGAAAACCCTGTTCCTACAAGAGCAGAGACCACGG
ACATCTCCAATGCAATAATCGATGGAACCGATGCGGTGATGCTCTCAGCCGAAACCTCTGTTGGCAAATATCCTATCGAA
GCAGTGAGTGTAATGCATAAAACCGCATTGTCCACAGAAAAGTATATTCGTGCCCACCCCTATCTTCTCCAATGGACTCG
CGAAGATGTTGTCACCGACGACCATACGGACGCTATATGCCGCGCGGCGTGGGATATAAGCGAAACATTAAAAATAAAGC
TTATCGTTTCGTCTACCTATACAGGGCACACGGCAATAAACGTTTCCGGTTTCAGACCACGTTCGCACATTCTGGCAGTT
ACCCCAAACAAGAATACGTACCACCGCCTTGGAATGGTCTGGGGTGTAACACCCATGCTATTAGAGCTCGGTTCAACGAT
AGACGAAATGGTGAAGATTGCCAGTGAAAAGGCCAGAAAGCTCGACCTTCTTGATCCGGGAGATAATTTCATAATCACTG
CTGGTGTTCCACTTGGCAAGGCTAACACTACCAATATGTTGAAACTCGAAAGAGCTTAA

Upstream 100 bases:

>100_bases
AGAATACCTTTGAAAAAGGTTCTTTCCGAGAAGAAAAAAATCGATGAAAAACTAATAGAAATGTCAAAAATCCTTTCGTA
GGATCGTAGGAGGAGACACC

Downstream 100 bases:

>100_bases
ATTGACAATTAACCCATAGAAATGAAACTAAGAGGATTGCTTGCATTTTTAATAGAAATACGTAGTTTTACTAGTATAAT
CGGCTTTATTTCCGCATATT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRKTKIVCTVGPATENPDMLKKLIEAGMNVVRLNTSHDDLEHHRRRIRTVKKIREELAVPITILLDLAGPKIRTGDFSSD
TVVLKKGDLFTLTTEDIVGDDKRVSVSYKKLPSEVNSGDFILVNDGKIKLKVVETNETEIKTTVVNGGEITHRRGINVPG
IDLGIEALTLKDKEFIKLGIEEGVDYFALSFVRKPEDVIEAKKIISSFGGSIPIISKIETVQALKRIESIAEVSDGLMVA
RGDLGVEIPVEEVPIAQKKIIRAGNKNRIPVITATQMLESMIENPVPTRAETTDISNAIIDGTDAVMLSAETSVGKYPIE
AVSVMHKTALSTEKYIRAHPYLLQWTREDVVTDDHTDAICRAAWDISETLKIKLIVSSTYTGHTAINVSGFRPRSHILAV
TPNKNTYHRLGMVWGVTPMLLELGSTIDEMVKIASEKARKLDLLDPGDNFIITAGVPLGKANTTNMLKLERA

Sequences:

>Translated_472_residues
MRKTKIVCTVGPATENPDMLKKLIEAGMNVVRLNTSHDDLEHHRRRIRTVKKIREELAVPITILLDLAGPKIRTGDFSSD
TVVLKKGDLFTLTTEDIVGDDKRVSVSYKKLPSEVNSGDFILVNDGKIKLKVVETNETEIKTTVVNGGEITHRRGINVPG
IDLGIEALTLKDKEFIKLGIEEGVDYFALSFVRKPEDVIEAKKIISSFGGSIPIISKIETVQALKRIESIAEVSDGLMVA
RGDLGVEIPVEEVPIAQKKIIRAGNKNRIPVITATQMLESMIENPVPTRAETTDISNAIIDGTDAVMLSAETSVGKYPIE
AVSVMHKTALSTEKYIRAHPYLLQWTREDVVTDDHTDAICRAAWDISETLKIKLIVSSTYTGHTAINVSGFRPRSHILAV
TPNKNTYHRLGMVWGVTPMLLELGSTIDEMVKIASEKARKLDLLDPGDNFIITAGVPLGKANTTNMLKLERA
>Mature_472_residues
MRKTKIVCTVGPATENPDMLKKLIEAGMNVVRLNTSHDDLEHHRRRIRTVKKIREELAVPITILLDLAGPKIRTGDFSSD
TVVLKKGDLFTLTTEDIVGDDKRVSVSYKKLPSEVNSGDFILVNDGKIKLKVVETNETEIKTTVVNGGEITHRRGINVPG
IDLGIEALTLKDKEFIKLGIEEGVDYFALSFVRKPEDVIEAKKIISSFGGSIPIISKIETVQALKRIESIAEVSDGLMVA
RGDLGVEIPVEEVPIAQKKIIRAGNKNRIPVITATQMLESMIENPVPTRAETTDISNAIIDGTDAVMLSAETSVGKYPIE
AVSVMHKTALSTEKYIRAHPYLLQWTREDVVTDDHTDAICRAAWDISETLKIKLIVSSTYTGHTAINVSGFRPRSHILAV
TPNKNTYHRLGMVWGVTPMLLELGSTIDEMVKIASEKARKLDLLDPGDNFIITAGVPLGKANTTNMLKLERA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=483, Percent_Identity=37.888198757764, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI33286422, Length=483, Percent_Identity=36.8530020703934, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI33286420, Length=483, Percent_Identity=36.8530020703934, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI10835121, Length=483, Percent_Identity=36.8530020703934, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI32967597, Length=483, Percent_Identity=36.8530020703934, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=40.0696864111498, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=40.0696864111498, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=36.7088607594937, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=36.7088607594937, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=35.3488372093023, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=42.6751592356688, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788160, Length=483, Percent_Identity=40.3726708074534, Blast_Score=301, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17544584, Length=479, Percent_Identity=36.9519832985386, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=36.1396303901437, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=36.1396303901437, Blast_Score=256, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=36.1396303901437, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=36.1396303901437, Blast_Score=255, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6319279, Length=482, Percent_Identity=36.7219917012448, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=36.3449691991786, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24648964, Length=484, Percent_Identity=36.7768595041322, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI28571814, Length=484, Percent_Identity=36.7768595041322, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24648966, Length=438, Percent_Identity=37.8995433789954, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24581235, Length=488, Percent_Identity=31.5573770491803, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=36.7647058823529, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51971; Mature: 51971

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVCTVGPATENPDMLKKLIEAGMNVVRLNTSHDDLEHHRRRIRTVKKIREELAVP
CCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
ITILLDLAGPKIRTGDFSSDTVVLKKGDLFTLTTEDIVGDDKRVSVSYKKLPSEVNSGDF
EEEEEECCCCEEECCCCCCCEEEEECCCEEEEEHHHHCCCCCEEEEEHHHCCCCCCCCCE
ILVNDGKIKLKVVETNETEIKTTVVNGGEITHRRGINVPGIDLGIEALTLKDKEFIKLGI
EEEECCEEEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCEEEEEECCHHHHEEEH
EEGVDYFALSFVRKPEDVIEAKKIISSFGGSIPIISKIETVQALKRIESIAEVSDGLMVA
HHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
RGDLGVEIPVEEVPIAQKKIIRAGNKNRIPVITATQMLESMIENPVPTRAETTDISNAII
ECCCCCCCCHHHCCCHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCHHEE
DGTDAVMLSAETSVGKYPIEAVSVMHKTALSTEKYIRAHPYLLQWTREDVVTDDHTDAIC
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHHHCCCEEEEECHHHCCCCCCCHHHH
RAAWDISETLKIKLIVSSTYTGHTAINVSGFRPRSHILAVTPNKNTYHRLGMVWGVTPML
HHHCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
LELGSTIDEMVKIASEKARKLDLLDPGDNFIITAGVPLGKANTTNMLKLERA
HHHCCHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MRKTKIVCTVGPATENPDMLKKLIEAGMNVVRLNTSHDDLEHHRRRIRTVKKIREELAVP
CCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
ITILLDLAGPKIRTGDFSSDTVVLKKGDLFTLTTEDIVGDDKRVSVSYKKLPSEVNSGDF
EEEEEECCCCEEECCCCCCCEEEEECCCEEEEEHHHHCCCCCEEEEEHHHCCCCCCCCCE
ILVNDGKIKLKVVETNETEIKTTVVNGGEITHRRGINVPGIDLGIEALTLKDKEFIKLGI
EEEECCEEEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCEEEEEECCHHHHEEEH
EEGVDYFALSFVRKPEDVIEAKKIISSFGGSIPIISKIETVQALKRIESIAEVSDGLMVA
HHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
RGDLGVEIPVEEVPIAQKKIIRAGNKNRIPVITATQMLESMIENPVPTRAETTDISNAII
ECCCCCCCCHHHCCCHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCHHEE
DGTDAVMLSAETSVGKYPIEAVSVMHKTALSTEKYIRAHPYLLQWTREDVVTDDHTDAIC
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHHHCCCEEEEECHHHCCCCCCCHHHH
RAAWDISETLKIKLIVSSTYTGHTAINVSGFRPRSHILAVTPNKNTYHRLGMVWGVTPML
HHHCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
LELGSTIDEMVKIASEKARKLDLLDPGDNFIITAGVPLGKANTTNMLKLERA
HHHCCHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]