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The map label for this gene is pfkA [H]

Identifier: 239617996

GI number: 239617996

Start: 1742693

End: 1743655

Strand: Direct

Name: pfkA [H]

Synonym: Kole_1624

Alternate gene names: 239617996

Gene position: 1742693-1743655 (Clockwise)

Preceding gene: 239617990

Following gene: 239617997

Centisome position: 75.7

GC content: 45.69

Gene sequence:

>963_bases
ATGATAAAGAAAATTGCTGTAATGACAAGTGGTGGGGATGCCCCGGGAATGAACGCTGCGATAAGGGCGGCTGTGAGAAC
TGCCGTCACTGAAAACATAGAGATTGTGGGAATTAAAAGAGGGTACTCCGGGCTTTTAGACGAGGATTTCATAGAGATGA
GCTACGCATCTGTTGGCGGGATTATGGAAAAGGGAGGTACAATTCTCAGGAGTTCCAGGTGCGAAGAGTTTCAAACCACT
GAAGGCAGGAAGAGAGCGGCAGAAATATTGAAAAAATACGGTATAGAAGCATTGGTAGTAATAGGAGGAGAAGGGAGTCT
CACCGGGGCCATGCTTCTTTCGGAAGAAAATGACATTCCCGTAATCGGCATCCCAGGGACGATAGACAACGATATAGCGC
ACACGGATATGTGTATCGGAGTAGATACCTGTTTGAATACCGTTATTGGCAACATTCAAAAACTCAAAGATACCGCTTCA
TCTCATGAAAGGGCCTTTATAGTAGAGGTTATGGGCAGGCATTCGGGTTATATCGCTCTTGCTTCCGGATTAGCAACGGG
CGCGGAAGCAATACTGATCCCGGAAATCCCGGTAGATTATGATGCCATAGCGGAAAAACTCTGGAACGAAAGGAAACGCG
GGAAAATAAATTGCATTGTCGTGGTTGCTGAAGGTGTGGCAAGTGCCTATACGGTCGCAAGGCATATGGAACATCGAATA
GGCTATGAGACGAGAATAACCATTCTTGGACACGTCCAGCGTGGTGGCTCTCCGACTGCTTTTGACCGCATCCTTGCTTC
AAGAATGGGTTACGAAGCGATAAAGGCGCTTCAGGAAGAAGACTTCGGCACAATGATTGCCCTCCAATCAGGAAAGATGG
TAAGAATACCTTTGAAAAAGGTTCTTTCCGAGAAGAAAAAAATCGATGAAAAACTAATAGAAATGTCAAAAATCCTTTCG
TAG

Upstream 100 bases:

>100_bases
AATCAACATAAAGCCTTAGCTTAAATGAGATAAGGCGATTAATGTGCTATAATCACGCCAGATTTTAGTATGCGAATACT
ATCGCAAGGAGGTATCAATA

Downstream 100 bases:

>100_bases
GATCGTAGGAGGAGACACCATGAGGAAGACGAAAATAGTTTGTACTGTTGGGCCGGCAACAGAAAATCCCGATATGTTAA
AAAAACTCATAGAAGCTGGC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIKKIAVMTSGGDAPGMNAAIRAAVRTAVTENIEIVGIKRGYSGLLDEDFIEMSYASVGGIMEKGGTILRSSRCEEFQTT
EGRKRAAEILKKYGIEALVVIGGEGSLTGAMLLSEENDIPVIGIPGTIDNDIAHTDMCIGVDTCLNTVIGNIQKLKDTAS
SHERAFIVEVMGRHSGYIALASGLATGAEAILIPEIPVDYDAIAEKLWNERKRGKINCIVVVAEGVASAYTVARHMEHRI
GYETRITILGHVQRGGSPTAFDRILASRMGYEAIKALQEEDFGTMIALQSGKMVRIPLKKVLSEKKKIDEKLIEMSKILS

Sequences:

>Translated_320_residues
MIKKIAVMTSGGDAPGMNAAIRAAVRTAVTENIEIVGIKRGYSGLLDEDFIEMSYASVGGIMEKGGTILRSSRCEEFQTT
EGRKRAAEILKKYGIEALVVIGGEGSLTGAMLLSEENDIPVIGIPGTIDNDIAHTDMCIGVDTCLNTVIGNIQKLKDTAS
SHERAFIVEVMGRHSGYIALASGLATGAEAILIPEIPVDYDAIAEKLWNERKRGKINCIVVVAEGVASAYTVARHMEHRI
GYETRITILGHVQRGGSPTAFDRILASRMGYEAIKALQEEDFGTMIALQSGKMVRIPLKKVLSEKKKIDEKLIEMSKILS
>Mature_320_residues
MIKKIAVMTSGGDAPGMNAAIRAAVRTAVTENIEIVGIKRGYSGLLDEDFIEMSYASVGGIMEKGGTILRSSRCEEFQTT
EGRKRAAEILKKYGIEALVVIGGEGSLTGAMLLSEENDIPVIGIPGTIDNDIAHTDMCIGVDTCLNTVIGNIQKLKDTAS
SHERAFIVEVMGRHSGYIALASGLATGAEAILIPEIPVDYDAIAEKLWNERKRGKINCIVVVAEGVASAYTVARHMEHRI
GYETRITILGHVQRGGSPTAFDRILASRMGYEAIKALQEEDFGTMIALQSGKMVRIPLKKVLSEKKKIDEKLIEMSKILS

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=356, Percent_Identity=40.7303370786517, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI266453768, Length=356, Percent_Identity=40.7303370786517, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI266453748, Length=356, Percent_Identity=40.7303370786517, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI4505749, Length=356, Percent_Identity=40.7303370786517, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI48762920, Length=348, Percent_Identity=40.5172413793103, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI11321601, Length=349, Percent_Identity=41.2607449856734, Blast_Score=246, Evalue=3e-65,
Organism=Escherichia coli, GI1790350, Length=307, Percent_Identity=46.2540716612378, Blast_Score=261, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI25147584, Length=349, Percent_Identity=40.1146131805158, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17558788, Length=355, Percent_Identity=35.7746478873239, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI72003601, Length=191, Percent_Identity=40.8376963350785, Blast_Score=144, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=333, Percent_Identity=40.2402402402402, Blast_Score=226, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6323861, Length=349, Percent_Identity=40.1146131805158, Blast_Score=224, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24652337, Length=349, Percent_Identity=42.9799426934097, Blast_Score=260, Evalue=9e-70,
Organism=Drosophila melanogaster, GI17647809, Length=349, Percent_Identity=42.9799426934097, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI28573326, Length=349, Percent_Identity=42.4068767908309, Blast_Score=257, Evalue=9e-69,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34582; Mature: 34582

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIAVMTSGGDAPGMNAAIRAAVRTAVTENIEIVGIKRGYSGLLDEDFIEMSYASVGG
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
IMEKGGTILRSSRCEEFQTTEGRKRAAEILKKYGIEALVVIGGEGSLTGAMLLSEENDIP
HHHCCCCEEHHCCCHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCC
VIGIPGTIDNDIAHTDMCIGVDTCLNTVIGNIQKLKDTASSHERAFIVEVMGRHSGYIAL
EEECCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEE
ASGLATGAEAILIPEIPVDYDAIAEKLWNERKRGKINCIVVVAEGVASAYTVARHMEHRI
ECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHC
GYETRITILGHVQRGGSPTAFDRILASRMGYEAIKALQEEDFGTMIALQSGKMVRIPLKK
CCEEEEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCEEEECHHH
VLSEKKKIDEKLIEMSKILS
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKKIAVMTSGGDAPGMNAAIRAAVRTAVTENIEIVGIKRGYSGLLDEDFIEMSYASVGG
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
IMEKGGTILRSSRCEEFQTTEGRKRAAEILKKYGIEALVVIGGEGSLTGAMLLSEENDIP
HHHCCCCEEHHCCCHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCC
VIGIPGTIDNDIAHTDMCIGVDTCLNTVIGNIQKLKDTASSHERAFIVEVMGRHSGYIAL
EEECCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEE
ASGLATGAEAILIPEIPVDYDAIAEKLWNERKRGKINCIVVVAEGVASAYTVARHMEHRI
ECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHC
GYETRITILGHVQRGGSPTAFDRILASRMGYEAIKALQEEDFGTMIALQSGKMVRIPLKK
CCEEEEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCEEEECHHH
VLSEKKKIDEKLIEMSKILS
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]