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The map label for this gene is ramA [H]

Identifier: 239617822

GI number: 239617822

Start: 1547514

End: 1548320

Strand: Direct

Name: ramA [H]

Synonym: Kole_1448

Alternate gene names: 239617822

Gene position: 1547514-1548320 (Clockwise)

Preceding gene: 239617821

Following gene: 239617823

Centisome position: 67.22

GC content: 41.02

Gene sequence:

>807_bases
ATGGGAAAAACGACAAAGATAACAATAGGAAGTGTTCAATTCAATCCTCTACAAAAAAACGGCGGCGAGAATCTGGAAAG
AGCTGTAAACTATATAGAACTTCTTGTAGAAAAAGGAGTAGATTTAGTGCTCTTGCCGGAGATGTTCAATACGGGTTATG
GAACAGACGATGAGACCCTGCAACTTGCAACCGAACTGTACGAAGAAACGCTGGAAACTCTATCGGCTATTGCCGATTAC
AATGATGTCGCTATTATAGGAGGAATGATTCGTAAAAAGACCGATAGATATTTCAATTCAACGGTGATTGTGCTTCCATA
TCGTGAACCGGTTTTTTACGACAAAACGCACCTTTTCCGAGATGAAAAGGGGGTATTTTCACCGGGAGAAAAATTTGTGA
CTTTTGTGTATCTCGATGTTACTTTTGGAGTAGTCATGTGTTATGAAATTGGATTTCCCGAGATCTCAAGAATTCTCTGC
AAACAAAGTGGTGCACAAGTTTTGCTGGCTCCTTTTGCTTTTGGAGCGGAAAGGCACAGAATATACGACGTTGCGACGAG
ATCAAGAGCGATGGAGAACGGAGCATTTTTGGTTACTTCCAGCCAAAACGGAAATTCAGGGAAAATGAATTTTATTGGAA
AAAGCCGTATTGTTTCTCCTTCAGGCGAAATAATAACAGAAGCCGGCAATGCTGAAGGCATCATCTTAGGAAAACTTGAT
TTGGGACTGGTGGAAAAGTATAGGTACAGGGAGGTTGATGATTCCCACGCTTATTATGCCAACTTTAGAAATGACCTCTA
CAAGTGA

Upstream 100 bases:

>100_bases
AGACTTTCCGCAAAAGGTATAATGAAGGGACAATGGCAGAATCAAATAACGATTTCCCAGGAAATTTCGCACAGTGAACC
AAAAGGAGAGTGAGTGATCA

Downstream 100 bases:

>100_bases
TTTGGGTATTGACAGTTAGGAATTTATGAGATACAATAGCATTCGGAAACTGCGGAAGGGCCGTTAGCTCAACAGGCAGA
GCGGCTGATTCTTAATCAGT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MGKTTKITIGSVQFNPLQKNGGENLERAVNYIELLVEKGVDLVLLPEMFNTGYGTDDETLQLATELYEETLETLSAIADY
NDVAIIGGMIRKKTDRYFNSTVIVLPYREPVFYDKTHLFRDEKGVFSPGEKFVTFVYLDVTFGVVMCYEIGFPEISRILC
KQSGAQVLLAPFAFGAERHRIYDVATRSRAMENGAFLVTSSQNGNSGKMNFIGKSRIVSPSGEIITEAGNAEGIILGKLD
LGLVEKYRYREVDDSHAYYANFRNDLYK

Sequences:

>Translated_268_residues
MGKTTKITIGSVQFNPLQKNGGENLERAVNYIELLVEKGVDLVLLPEMFNTGYGTDDETLQLATELYEETLETLSAIADY
NDVAIIGGMIRKKTDRYFNSTVIVLPYREPVFYDKTHLFRDEKGVFSPGEKFVTFVYLDVTFGVVMCYEIGFPEISRILC
KQSGAQVLLAPFAFGAERHRIYDVATRSRAMENGAFLVTSSQNGNSGKMNFIGKSRIVSPSGEIITEAGNAEGIILGKLD
LGLVEKYRYREVDDSHAYYANFRNDLYK
>Mature_267_residues
GKTTKITIGSVQFNPLQKNGGENLERAVNYIELLVEKGVDLVLLPEMFNTGYGTDDETLQLATELYEETLETLSAIADYN
DVAIIGGMIRKKTDRYFNSTVIVLPYREPVFYDKTHLFRDEKGVFSPGEKFVTFVYLDVTFGVVMCYEIGFPEISRILCK
QSGAQVLLAPFAFGAERHRIYDVATRSRAMENGAFLVTSSQNGNSGKMNFIGKSRIVSPSGEIITEAGNAEGIILGKLDL
GLVEKYRYREVDDSHAYYANFRNDLYK

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=261, Percent_Identity=28.735632183908, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI5031947, Length=266, Percent_Identity=27.0676691729323, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI297632350, Length=266, Percent_Identity=27.0676691729323, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI297632348, Length=266, Percent_Identity=27.0676691729323, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1786412, Length=214, Percent_Identity=27.5700934579439, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556280, Length=267, Percent_Identity=26.5917602996255, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=265, Percent_Identity=30.188679245283, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355835, Length=269, Percent_Identity=30.1115241635688, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 30062; Mature: 29930

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTKITIGSVQFNPLQKNGGENLERAVNYIELLVEKGVDLVLLPEMFNTGYGTDDETL
CCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCHHHH
QLATELYEETLETLSAIADYNDVAIIGGMIRKKTDRYFNSTVIVLPYREPVFYDKTHLFR
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCEEEEEECCCCCEECHHHHEE
DEKGVFSPGEKFVTFVYLDVTFGVVMCYEIGFPEISRILCKQSGAQVLLAPFAFGAERHR
CCCCCCCCCCCEEEEEEEEHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCHHE
IYDVATRSRAMENGAFLVTSSQNGNSGKMNFIGKSRIVSPSGEIITEAGNAEGIILGKLD
EEHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCEEECCCCCEEEECCCCCEEEEEECC
LGLVEKYRYREVDDSHAYYANFRNDLYK
HHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GKTTKITIGSVQFNPLQKNGGENLERAVNYIELLVEKGVDLVLLPEMFNTGYGTDDETL
CCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCHHHH
QLATELYEETLETLSAIADYNDVAIIGGMIRKKTDRYFNSTVIVLPYREPVFYDKTHLFR
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCEEEEEECCCCCEECHHHHEE
DEKGVFSPGEKFVTFVYLDVTFGVVMCYEIGFPEISRILCKQSGAQVLLAPFAFGAERHR
CCCCCCCCCCCEEEEEEEEHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCHHE
IYDVATRSRAMENGAFLVTSSQNGNSGKMNFIGKSRIVSPSGEIITEAGNAEGIILGKLD
EEHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCEEECCCCCEEEECCCCCEEEEEECC
LGLVEKYRYREVDDSHAYYANFRNDLYK
HHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA