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The map label for this gene is ispG [H]

Identifier: 239617302

GI number: 239617302

Start: 963136

End: 964179

Strand: Direct

Name: ispG [H]

Synonym: Kole_0911

Alternate gene names: 239617302

Gene position: 963136-964179 (Clockwise)

Preceding gene: 239617301

Following gene: 239617303

Centisome position: 41.84

GC content: 42.72

Gene sequence:

>1044_bases
GTGAGCAGAATAATAAAAATTGGGAATGTTGAAATAGGCGGAAATAACCCTATCGTAATTCAATCTATGACAAATACCAA
GACCGCAGACGTAAATGCCACTATCTCGCAAATAAGAGCTCTCTCATCAGCTGGATGCGGGATAGTCAGAGTATCTGTCC
CGGATTTTGAGAGTATAAAAAGCCTGAAAGAGATAGTTAAGAACGTAAAGATCCCGATTGTTGCCGATATCCATTTTGAC
TATCGTCTCGCCATAGAATCTATAAAAGCCGGTGCCTCCAAGATTCGAATAAATCCGGGAAACATAGGTGCTGACTGGAA
GGTCAGGGAAGTTGTTAGGGTAGCAAAAGAATATGGCGTTCCCATAAGGGTAGGGGCGAATTCCGGGTCCATAAAAAAAG
GTTACACTCATCTTCAAAAAGCTGAAGCTCTTGCCGAAAGTGCCCTGGAAGAAGTGAGAATTCTTGAATCCATGGGGTTT
GATAGAATAGTCATCTCCGCAAAGTCTTCATCTGTCATGGAAACTATCGAGGCGAATGTTTATATCCACTCTAAAGTTGA
TTATCCACTTCATATAGGTGTTACTGAAGCCGGTGTTGGTGAGAGCGCGATCGTGAAATCATCCATAGGGATTGGGGCAT
TGCTCATCAAAGGAATTGGAGACACTATAAGAGTTTCTATGGCTGGGGATCCGATAAAGGAAGTTCTCATAGCAAAAGAA
ATCCTTAAATCACTTGGTAAGTTGAAAGGGCCGCAGGTGATTGCCTGTCCCACCTGCGCGCGTACCGAGATAGAGGTAGA
AGTTCTTGCAAAAGAAGTAACAGAATGGCTGGAAGGAATAGATGAGGAACTCACAGTAGCTGTCATGGGTTGTGTTGTAA
ATGGTATCGGCGAAGGAAAACATGCGGATATAGGTGTGACAGGAACAAAAGATCAAGCCGTTATATTCATTAAGGGGAAA
ATCGTGGAACAGGTGGAAAAAGGTAAACTAAAGGAAAGGTTCTTCTTTCACCTGGAGAATTTATTGGGGAGGAAAAAAAC
ATGA

Upstream 100 bases:

>100_bases
GAATTGATCCAAAGGTAGAAGGTTATATTCACACAATCGGGTTTATAATAATTATGGGAATTTTAATCTACATAACCTTT
ATAGATATAGGAAGATTTCT

Downstream 100 bases:

>100_bases
AGGTATCCATAATTGGTGCGGGAATGGTTGGATCGTCAATCGCCTATGCAACGATGATAAAAGGGGTAGCAAGGGAAATA
AGCATCGTGGATATAAACGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSRIIKIGNVEIGGNNPIVIQSMTNTKTADVNATISQIRALSSAGCGIVRVSVPDFESIKSLKEIVKNVKIPIVADIHFD
YRLAIESIKAGASKIRINPGNIGADWKVREVVRVAKEYGVPIRVGANSGSIKKGYTHLQKAEALAESALEEVRILESMGF
DRIVISAKSSSVMETIEANVYIHSKVDYPLHIGVTEAGVGESAIVKSSIGIGALLIKGIGDTIRVSMAGDPIKEVLIAKE
ILKSLGKLKGPQVIACPTCARTEIEVEVLAKEVTEWLEGIDEELTVAVMGCVVNGIGEGKHADIGVTGTKDQAVIFIKGK
IVEQVEKGKLKERFFFHLENLLGRKKT

Sequences:

>Translated_347_residues
MSRIIKIGNVEIGGNNPIVIQSMTNTKTADVNATISQIRALSSAGCGIVRVSVPDFESIKSLKEIVKNVKIPIVADIHFD
YRLAIESIKAGASKIRINPGNIGADWKVREVVRVAKEYGVPIRVGANSGSIKKGYTHLQKAEALAESALEEVRILESMGF
DRIVISAKSSSVMETIEANVYIHSKVDYPLHIGVTEAGVGESAIVKSSIGIGALLIKGIGDTIRVSMAGDPIKEVLIAKE
ILKSLGKLKGPQVIACPTCARTEIEVEVLAKEVTEWLEGIDEELTVAVMGCVVNGIGEGKHADIGVTGTKDQAVIFIKGK
IVEQVEKGKLKERFFFHLENLLGRKKT
>Mature_346_residues
SRIIKIGNVEIGGNNPIVIQSMTNTKTADVNATISQIRALSSAGCGIVRVSVPDFESIKSLKEIVKNVKIPIVADIHFDY
RLAIESIKAGASKIRINPGNIGADWKVREVVRVAKEYGVPIRVGANSGSIKKGYTHLQKAEALAESALEEVRILESMGFD
RIVISAKSSSVMETIEANVYIHSKVDYPLHIGVTEAGVGESAIVKSSIGIGALLIKGIGDTIRVSMAGDPIKEVLIAKEI
LKSLGKLKGPQVIACPTCARTEIEVEVLAKEVTEWLEGIDEELTVAVMGCVVNGIGEGKHADIGVTGTKDQAVIFIKGKI
VEQVEKGKLKERFFFHLENLLGRKKT

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=322, Percent_Identity=44.7204968944099, Blast_Score=277, Evalue=7e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37317; Mature: 37186

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIIKIGNVEIGGNNPIVIQSMTNTKTADVNATISQIRALSSAGCGIVRVSVPDFESIK
CCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHH
SLKEIVKNVKIPIVADIHFDYRLAIESIKAGASKIRINPGNIGADWKVREVVRVAKEYGV
HHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCC
PIRVGANSGSIKKGYTHLQKAEALAESALEEVRILESMGFDRIVISAKSSSVMETIEANV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCE
YIHSKVDYPLHIGVTEAGVGESAIVKSSIGIGALLIKGIGDTIRVSMAGDPIKEVLIAKE
EEEEECCCEEEECCCCCCCCCHHHHHHCCCHHHHEEECCCCEEEEEECCCHHHHHHHHHH
ILKSLGKLKGPQVIACPTCARTEIEVEVLAKEVTEWLEGIDEELTVAVMGCVVNGIGEGK
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
HADIGVTGTKDQAVIFIKGKIVEQVEKGKLKERFFFHLENLLGRKKT
CCEEEEECCCCCEEEEEECHHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRIIKIGNVEIGGNNPIVIQSMTNTKTADVNATISQIRALSSAGCGIVRVSVPDFESIK
CCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHH
SLKEIVKNVKIPIVADIHFDYRLAIESIKAGASKIRINPGNIGADWKVREVVRVAKEYGV
HHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCC
PIRVGANSGSIKKGYTHLQKAEALAESALEEVRILESMGFDRIVISAKSSSVMETIEANV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCE
YIHSKVDYPLHIGVTEAGVGESAIVKSSIGIGALLIKGIGDTIRVSMAGDPIKEVLIAKE
EEEEECCCEEEECCCCCCCCCHHHHHHCCCHHHHEEECCCCEEEEEECCCHHHHHHHHHH
ILKSLGKLKGPQVIACPTCARTEIEVEVLAKEVTEWLEGIDEELTVAVMGCVVNGIGEGK
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
HADIGVTGTKDQAVIFIKGKIVEQVEKGKLKERFFFHLENLLGRKKT
CCEEEEECCCCCEEEEEECHHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA