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The map label for this gene is ldh

Identifier: 239617303

GI number: 239617303

Start: 964176

End: 965147

Strand: Direct

Name: ldh

Synonym: Kole_0912

Alternate gene names: 239617303

Gene position: 964176-965147 (Clockwise)

Preceding gene: 239617302

Following gene: 239617304

Centisome position: 41.88

GC content: 42.8

Gene sequence:

>972_bases
ATGAAGGTATCCATAATTGGTGCGGGAATGGTTGGATCGTCAATCGCCTATGCAACGATGATAAAAGGGGTAGCAAGGGA
AATAAGCATCGTGGATATAAACGGTGATTTAGCGGAAGGTCAGGCTCTCGACTTGAGTCATGGAGCTCCCTATGTTCATC
CTGTAAGGATAAAAGGCGGCAATGATTACAGTTTAACGAAAAATTCCGATGTTGTCGTTATCACTGCCGGTAGAGCTCAA
AAACCTGGTGAAACGAGATTACAGCTTCTTAAAAGTAATGCGAAGATCATCTCTTCGATAGTTGAAAGCTGTCTCAAATA
TTCCGAAAATCCGATAATACTGATGGTCTCAAATCCCGTAGATGTGCTTACGTGGGTAGCGTGGAAGAAATCCGGGCTAC
CAAGAGAACGAATTATCGGTTCAGGTACGACACTTGATACGGCAAGGCTCAGACAGAATATAGCAGATCATTGCAAACTT
GACCCGAGAAGTGTACATGCCTATATTATAGGAGAACATGGGGATAGTGAAATCGCCAGTTGGTCCACGGCGAATGTGGG
AGGAGTTCCAATAAAAGAATTCTGCAATGGCTGCTTGGCTAAAGGGTGCGAAAGAGATAAGGTGTTCGAAAGAATATTCG
AGAATACCCGTGATGCTGCTTATAAAATCATTGAAAAGAAAGGCGCTACATACTATGGTATCGGCCTTGCTGTGGCAAGA
ATTCTTGAAACTATCGCTGGAGATCATCATTCTGTCTTAACGGTATCCAGTGTCCATGAAGAGTTTAGAGGAATGCGGGA
TGTTCCTTTTAGTGTTCCCAGTGTTCTGGGTAAAAAAGGAATAGAAAGAATCCTTCCTTTAAAACTCTCGGACGATGAAT
TGAAAGGATTAGAAAATTCCGCGAAAGTTATAAAAGCTGCTATAGAAAGCATATTAGAGAATCGAGAGCCGAGAGCCGAG
AATCCGAGATAG

Upstream 100 bases:

>100_bases
TTATATTCATTAAGGGGAAAATCGTGGAACAGGTGGAAAAAGGTAAACTAAAGGAAAGGTTCTTCTTTCACCTGGAGAAT
TTATTGGGGAGGAAAAAAAC

Downstream 100 bases:

>100_bases
CAGCTACGCTTGAAGTGCGGAGCTGCGCTCCGGACGCTCACGAGGTTGAGGTTTGCAGACGCTGTGCGCTGCTTCGCAGC
GGTTGTGCTGGGCTTTGCCC

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKVSIIGAGMVGSSIAYATMIKGVAREISIVDINGDLAEGQALDLSHGAPYVHPVRIKGGNDYSLTKNSDVVVITAGRAQ
KPGETRLQLLKSNAKIISSIVESCLKYSENPIILMVSNPVDVLTWVAWKKSGLPRERIIGSGTTLDTARLRQNIADHCKL
DPRSVHAYIIGEHGDSEIASWSTANVGGVPIKEFCNGCLAKGCERDKVFERIFENTRDAAYKIIEKKGATYYGIGLAVAR
ILETIAGDHHSVLTVSSVHEEFRGMRDVPFSVPSVLGKKGIERILPLKLSDDELKGLENSAKVIKAAIESILENREPRAE
NPR

Sequences:

>Translated_323_residues
MKVSIIGAGMVGSSIAYATMIKGVAREISIVDINGDLAEGQALDLSHGAPYVHPVRIKGGNDYSLTKNSDVVVITAGRAQ
KPGETRLQLLKSNAKIISSIVESCLKYSENPIILMVSNPVDVLTWVAWKKSGLPRERIIGSGTTLDTARLRQNIADHCKL
DPRSVHAYIIGEHGDSEIASWSTANVGGVPIKEFCNGCLAKGCERDKVFERIFENTRDAAYKIIEKKGATYYGIGLAVAR
ILETIAGDHHSVLTVSSVHEEFRGMRDVPFSVPSVLGKKGIERILPLKLSDDELKGLENSAKVIKAAIESILENREPRAE
NPR
>Mature_323_residues
MKVSIIGAGMVGSSIAYATMIKGVAREISIVDINGDLAEGQALDLSHGAPYVHPVRIKGGNDYSLTKNSDVVVITAGRAQ
KPGETRLQLLKSNAKIISSIVESCLKYSENPIILMVSNPVDVLTWVAWKKSGLPRERIIGSGTTLDTARLRQNIADHCKL
DPRSVHAYIIGEHGDSEIASWSTANVGGVPIKEFCNGCLAKGCERDKVFERIFENTRDAAYKIIEKKGATYYGIGLAVAR
ILETIAGDHHSVLTVSSVHEEFRGMRDVPFSVPSVLGKKGIERILPLKLSDDELKGLENSAKVIKAAIESILENREPRAE
NPR

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=39.672131147541, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=38.6885245901639, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=38.6885245901639, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=38.0327868852459, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=38.0327868852459, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=38.0327868852459, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=38.0327868852459, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=38.6885245901639, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=38.6885245901639, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI260099725, Length=228, Percent_Identity=40.3508771929825, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=41.1764705882353, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI207028494, Length=192, Percent_Identity=38.0208333333333, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI103472011, Length=308, Percent_Identity=24.3506493506494, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=28.021978021978, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI21735621, Length=325, Percent_Identity=26.7692307692308, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789632, Length=298, Percent_Identity=29.5302013422819, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535107, Length=290, Percent_Identity=41.0344827586207, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6320125, Length=265, Percent_Identity=28.3018867924528, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322765, Length=313, Percent_Identity=26.517571884984, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=38.1578947368421, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=30.8196721311475, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24647881, Length=321, Percent_Identity=28.6604361370716, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24663599, Length=323, Percent_Identity=24.4582043343653, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH_KOSOT (C5CGP2)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002940625.1
- ProteinModelPortal:   C5CGP2
- GeneID:   7967453
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0912
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 35082; Mature: 35082

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 173-173 BINDING 86-86 BINDING 118-118 BINDING 149-149 BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSIIGAGMVGSSIAYATMIKGVAREISIVDINGDLAEGQALDLSHGAPYVHPVRIKGG
CEEEEEECCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCCCCEEEEEEECC
NDYSLTKNSDVVVITAGRAQKPGETRLQLLKSNAKIISSIVESCLKYSENPIILMVSNPV
CCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
DVLTWVAWKKSGLPRERIIGSGTTLDTARLRQNIADHCKLDPRSVHAYIIGEHGDSEIAS
HHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
WSTANVGGVPIKEFCNGCLAKGCERDKVFERIFENTRDAAYKIIEKKGATYYGIGLAVAR
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
ILETIAGDHHSVLTVSSVHEEFRGMRDVPFSVPSVLGKKGIERILPLKLSDDELKGLENS
HHHHHCCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHH
AKVIKAAIESILENREPRAENPR
HHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKVSIIGAGMVGSSIAYATMIKGVAREISIVDINGDLAEGQALDLSHGAPYVHPVRIKGG
CEEEEEECCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCCCCEEEEEEECC
NDYSLTKNSDVVVITAGRAQKPGETRLQLLKSNAKIISSIVESCLKYSENPIILMVSNPV
CCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
DVLTWVAWKKSGLPRERIIGSGTTLDTARLRQNIADHCKLDPRSVHAYIIGEHGDSEIAS
HHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCC
WSTANVGGVPIKEFCNGCLAKGCERDKVFERIFENTRDAAYKIIEKKGATYYGIGLAVAR
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
ILETIAGDHHSVLTVSSVHEEFRGMRDVPFSVPSVLGKKGIERILPLKLSDDELKGLENS
HHHHHCCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHH
AKVIKAAIESILENREPRAENPR
HHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA