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The map label for this gene is trpB2 [H]

Identifier: 239617093

GI number: 239617093

Start: 749857

End: 751131

Strand: Direct

Name: trpB2 [H]

Synonym: Kole_0695

Alternate gene names: 239617093

Gene position: 749857-751131 (Clockwise)

Preceding gene: 239617058

Following gene: 239617095

Centisome position: 32.57

GC content: 46.04

Gene sequence:

>1275_bases
ATGCGTTCGAGAATCAGAGTAAATCTTCCGGTTGAGGAGATGCCGAAAAAATGGTACAACGTGAAAGCTGATCTACCTTT
CAAGCTCGATCCACCACTGCATCCAGCGACTCAGCAGCCGATGAAACCGGAGGAACTTTCGAGGATATTCCCTCCCTCCA
TTGTTGAACAGGAAGCAACTGAAGAAAGATTTGTAGAAATTCCAGAAGAAGTCCTGGAAGAGTATGTGGTCTTCAGGCCC
ACACCTCTAATAAGAGCTACCTTCCTTGAGGAATATCTCCAGACGCCTGCTCGCATTTATTACAAATATGAAGGCACCTC
ACCAACGGGAAGCCACAAAACCAACACCGCCCTCGCTCAAGCTTTTTACAACGCGAAAGATGGAATCAAACGGCTCGTTA
CCGAAACGGGAGCAGGTCAATGGGGAAGCGCGTTGTCATATGCTGGAAGAAAGTTTGGGTTGGAAGTCAAGGTTTTCATG
GTGCGTATCAGTTACGAACAAAAACCGATGAGAAAATACATGATGAACCTATTTGATGGAACTGTCACACCGAGTCCAAG
TGAACTCACTTCAAGCGGGAAGAAATTTCTGTCGCAAGATCCCTCACATCCCGGGTCTCTTGGTATTGCTATAAGCGAAG
CGATCGAAGAGGTTATGGCTGATCCCAATACAAGGTACTCGCTCGGAAGCGTTCTAGATCACGTTCTTTTACACCAAACG
GTTATTGGATTAGAAATAGAAAAACAGCTGGAACTGATTGGCGAAAAGCCAACGATTGTTATGGGCTGCCACGGTGGCGG
CTCGAATTTCGGAGGAACGGTTCTCCCATTCATTCCAAGAAAGCTTGCTGGAGAGAATATCCGGTTTGTGGCTTGTGAAC
CGGAATCCTGTCCGACATTGACAAAGGGTGAATTCAGGTACGATTCCGGAGATTCAGCAGGTTTTACACCGCTGATCAAG
ATGTACACTCTCGGAAAAGAATTCGTACCGCCAAAAATCCATGCTGGAGGACTTAGATACCACGGGTCAGCCCCAATCAT
AGCGCGTTTAGTTCACGAAGGGATAATTGAAGCAGAAAGCTTTGATCAAGAAAAAACTTTCGAAGCTGCAAGGCTCTTTG
CAAAATTGGAAGGAATTATCCCAGCACCAGAAGCCGCCCATGCAATAGCAGGAGCCATAAAAGAAGCAATAGCTGCTAAA
GAGGAAAAACGTGAAGAAGTAATAGTTTTTACTCTCAGTGGACACGGGTTAATGGATCTTACAGCATACGTTTGA

Upstream 100 bases:

>100_bases
AAGCCCCTTAATGGGGTAGTCCTGCTGTGCTGAAATTAGGATTACCCATAATTAAGGGGCTCTCTAAACAGAGAGCCCCT
ATTTTTTTGGAGGTGAAAAG

Downstream 100 bases:

>100_bases
AAGCTGTAAATCCTGGTAATCACAGAAAGGAGGAGCTTAATAAGCTCCTCCTTGTTGATTTTTTCAGCTCAATCATTTTC
TGTCCAGAGGATCACGGATA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRSRIRVNLPVEEMPKKWYNVKADLPFKLDPPLHPATQQPMKPEELSRIFPPSIVEQEATEERFVEIPEEVLEEYVVFRP
TPLIRATFLEEYLQTPARIYYKYEGTSPTGSHKTNTALAQAFYNAKDGIKRLVTETGAGQWGSALSYAGRKFGLEVKVFM
VRISYEQKPMRKYMMNLFDGTVTPSPSELTSSGKKFLSQDPSHPGSLGIAISEAIEEVMADPNTRYSLGSVLDHVLLHQT
VIGLEIEKQLELIGEKPTIVMGCHGGGSNFGGTVLPFIPRKLAGENIRFVACEPESCPTLTKGEFRYDSGDSAGFTPLIK
MYTLGKEFVPPKIHAGGLRYHGSAPIIARLVHEGIIEAESFDQEKTFEAARLFAKLEGIIPAPEAAHAIAGAIKEAIAAK
EEKREEVIVFTLSGHGLMDLTAYV

Sequences:

>Translated_424_residues
MRSRIRVNLPVEEMPKKWYNVKADLPFKLDPPLHPATQQPMKPEELSRIFPPSIVEQEATEERFVEIPEEVLEEYVVFRP
TPLIRATFLEEYLQTPARIYYKYEGTSPTGSHKTNTALAQAFYNAKDGIKRLVTETGAGQWGSALSYAGRKFGLEVKVFM
VRISYEQKPMRKYMMNLFDGTVTPSPSELTSSGKKFLSQDPSHPGSLGIAISEAIEEVMADPNTRYSLGSVLDHVLLHQT
VIGLEIEKQLELIGEKPTIVMGCHGGGSNFGGTVLPFIPRKLAGENIRFVACEPESCPTLTKGEFRYDSGDSAGFTPLIK
MYTLGKEFVPPKIHAGGLRYHGSAPIIARLVHEGIIEAESFDQEKTFEAARLFAKLEGIIPAPEAAHAIAGAIKEAIAAK
EEKREEVIVFTLSGHGLMDLTAYV
>Mature_424_residues
MRSRIRVNLPVEEMPKKWYNVKADLPFKLDPPLHPATQQPMKPEELSRIFPPSIVEQEATEERFVEIPEEVLEEYVVFRP
TPLIRATFLEEYLQTPARIYYKYEGTSPTGSHKTNTALAQAFYNAKDGIKRLVTETGAGQWGSALSYAGRKFGLEVKVFM
VRISYEQKPMRKYMMNLFDGTVTPSPSELTSSGKKFLSQDPSHPGSLGIAISEAIEEVMADPNTRYSLGSVLDHVLLHQT
VIGLEIEKQLELIGEKPTIVMGCHGGGSNFGGTVLPFIPRKLAGENIRFVACEPESCPTLTKGEFRYDSGDSAGFTPLIK
MYTLGKEFVPPKIHAGGLRYHGSAPIIARLVHEGIIEAESFDQEKTFEAARLFAKLEGIIPAPEAAHAIAGAIKEAIAAK
EEKREEVIVFTLSGHGLMDLTAYV

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=370, Percent_Identity=28.9189189189189, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321412, Length=358, Percent_Identity=30.1675977653631, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 46971; Mature: 46971

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRIRVNLPVEEMPKKWYNVKADLPFKLDPPLHPATQQPMKPEELSRIFPPSIVEQEAT
CCCCEEEECCHHHHCHHHHCCCCCCCEECCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHH
EERFVEIPEEVLEEYVVFRPTPLIRATFLEEYLQTPARIYYKYEGTSPTGSHKTNTALAQ
HHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHH
AFYNAKDGIKRLVTETGAGQWGSALSYAGRKFGLEVKVFMVRISYEQKPMRKYMMNLFDG
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHCCCEEEEEEEEECCCCCHHHHHHHHHHCC
TVTPSPSELTSSGKKFLSQDPSHPGSLGIAISEAIEEVMADPNTRYSLGSVLDHVLLHQT
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VIGLEIEKQLELIGEKPTIVMGCHGGGSNFGGTVLPFIPRKLAGENIRFVACEPESCPTL
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHCCHHHCCCCEEEEEECCCCCCCC
TKGEFRYDSGDSAGFTPLIKMYTLGKEFVPPKIHAGGLRYHGSAPIIARLVHEGIIEAES
CCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHC
FDQEKTFEAARLFAKLEGIIPAPEAAHAIAGAIKEAIAAKEEKREEVIVFTLSGHGLMDL
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
TAYV
EECC
>Mature Secondary Structure
MRSRIRVNLPVEEMPKKWYNVKADLPFKLDPPLHPATQQPMKPEELSRIFPPSIVEQEAT
CCCCEEEECCHHHHCHHHHCCCCCCCEECCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHH
EERFVEIPEEVLEEYVVFRPTPLIRATFLEEYLQTPARIYYKYEGTSPTGSHKTNTALAQ
HHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHH
AFYNAKDGIKRLVTETGAGQWGSALSYAGRKFGLEVKVFMVRISYEQKPMRKYMMNLFDG
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHCCCEEEEEEEEECCCCCHHHHHHHHHHCC
TVTPSPSELTSSGKKFLSQDPSHPGSLGIAISEAIEEVMADPNTRYSLGSVLDHVLLHQT
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VIGLEIEKQLELIGEKPTIVMGCHGGGSNFGGTVLPFIPRKLAGENIRFVACEPESCPTL
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHCCHHHCCCCEEEEEECCCCCCCC
TKGEFRYDSGDSAGFTPLIKMYTLGKEFVPPKIHAGGLRYHGSAPIIARLVHEGIIEAES
CCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHC
FDQEKTFEAARLFAKLEGIIPAPEAAHAIAGAIKEAIAAKEEKREEVIVFTLSGHGLMDL
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
TAYV
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]