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The map label for this gene is mdh [H]

Identifier: 239616891

GI number: 239616891

Start: 526135

End: 527256

Strand: Direct

Name: mdh [H]

Synonym: Kole_0486

Alternate gene names: 239616891

Gene position: 526135-527256 (Clockwise)

Preceding gene: 239616889

Following gene: 239616892

Centisome position: 22.85

GC content: 44.56

Gene sequence:

>1122_bases
ATGGAAGTAGTTTACGACGATGTTGTCTGGTTGGATTTTGACGTTCTCGAAAACTTTATGGTCGATGTTTTTAAAGCTCT
TGGAGTTCCGGAAGAAGATGCCAAAATAGTCGCAAATGTGCTTATTACGGCTGACAAACAAGGTATCGACTCCCACGGAG
TCGGCAGACTTAAGCCAATTTATGTCGAAAGAATTCAAGCGGGAATCCAGAACCCCATCACAAACTTTGAAATCGTCAGA
GAAAGCCCGACAACGGCGGTAGTAGATGGGCACAACGGCATGGGACATGTAATAGGCTACAAAGCCATGAAACTGGCCAT
AGAAAAAGCAAAAAAGTACGGCATGGGAATGGTCGCAGTAAGAAACTCTACCCATTATGGTATTGCTGGATACTATGTCC
AGATGGCTGTAGATGAAGGCATGATAGGTATCACCGGTACTAATGCGAGGCCATCCATAGCCCCCACCTTTGGCGTAGAA
CCCATGCTTGGGACAAACCCATTGACCTTCGGAATGCCTTCAGACGAAGAATTTCCCTTCATTCTTGACTGTGCCACGAG
TGTCTCTCAGAGAGGAAAAATCGAAGTTTACGCCAGAGCAGGTAAAGAAATTCCTGAAGGCTGGGTAATAGGAGAAGACG
GAAAAACCAGAACGGATACCGAACAAATCCTTGAAGACCTGAAAAAAGGAAAAGCCGCACTGGTTCCACTCGGTGGAATA
GGAGAAGAAACAGCCGGATACAAAGGTTACGGTTACGCCACTGTAGTTGAAATACTCTCCGCTGCCCTTCAGGGTGGAAA
CTTTCTCAAAATGCTGACAGGCTTCGATGAACAGGGAAATCGTGTCCCGTATAGAATCGGCCATTTCTTTATTGCAATCA
ATATCGAGGCCTTTACCGAATTAGAAAACTTCAAAAAAACAACGGGTGAAATACTGAGAGCTTTGAGAAACTCAAAAAAA
GCGCCGGGGGCTGAAAGAATATACACCGCCGGCGAAAAAGAATACCTGACATGGTTAGAAAGAAAAGATAAAGGTGCTCC
ACTAAACCACGTTCTCAGAGAACAACTCGTTCAATTAAGAGATGAATTCAATTTAGACCAGTATAAATTCCCCTGGGAAT
GA

Upstream 100 bases:

>100_bases
TCCGCTGCGAAGCAGCCCTCTCACTCAGTTCTCGTTTTTTTCTGTCCTCATGTTATATTTGAAACGTAAATAAGTTTTGA
AAAAAAGGAGATGTTCGTAC

Downstream 100 bases:

>100_bases
TTATAAAAACCAGAAACATGCGGAGGGATAAAAATGGATCTCAAAGAAAAAGCACTTAAACAGCATGAATTATGGCAGGG
AAAACTCGAGGTTATTTCCA

Product: Malate/L-lactate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MEVVYDDVVWLDFDVLENFMVDVFKALGVPEEDAKIVANVLITADKQGIDSHGVGRLKPIYVERIQAGIQNPITNFEIVR
ESPTTAVVDGHNGMGHVIGYKAMKLAIEKAKKYGMGMVAVRNSTHYGIAGYYVQMAVDEGMIGITGTNARPSIAPTFGVE
PMLGTNPLTFGMPSDEEFPFILDCATSVSQRGKIEVYARAGKEIPEGWVIGEDGKTRTDTEQILEDLKKGKAALVPLGGI
GEETAGYKGYGYATVVEILSAALQGGNFLKMLTGFDEQGNRVPYRIGHFFIAINIEAFTELENFKKTTGEILRALRNSKK
APGAERIYTAGEKEYLTWLERKDKGAPLNHVLREQLVQLRDEFNLDQYKFPWE

Sequences:

>Translated_373_residues
MEVVYDDVVWLDFDVLENFMVDVFKALGVPEEDAKIVANVLITADKQGIDSHGVGRLKPIYVERIQAGIQNPITNFEIVR
ESPTTAVVDGHNGMGHVIGYKAMKLAIEKAKKYGMGMVAVRNSTHYGIAGYYVQMAVDEGMIGITGTNARPSIAPTFGVE
PMLGTNPLTFGMPSDEEFPFILDCATSVSQRGKIEVYARAGKEIPEGWVIGEDGKTRTDTEQILEDLKKGKAALVPLGGI
GEETAGYKGYGYATVVEILSAALQGGNFLKMLTGFDEQGNRVPYRIGHFFIAINIEAFTELENFKKTTGEILRALRNSKK
APGAERIYTAGEKEYLTWLERKDKGAPLNHVLREQLVQLRDEFNLDQYKFPWE
>Mature_373_residues
MEVVYDDVVWLDFDVLENFMVDVFKALGVPEEDAKIVANVLITADKQGIDSHGVGRLKPIYVERIQAGIQNPITNFEIVR
ESPTTAVVDGHNGMGHVIGYKAMKLAIEKAKKYGMGMVAVRNSTHYGIAGYYVQMAVDEGMIGITGTNARPSIAPTFGVE
PMLGTNPLTFGMPSDEEFPFILDCATSVSQRGKIEVYARAGKEIPEGWVIGEDGKTRTDTEQILEDLKKGKAALVPLGGI
GEETAGYKGYGYATVVEILSAALQGGNFLKMLTGFDEQGNRVPYRIGHFFIAINIEAFTELENFKKTTGEILRALRNSKK
APGAERIYTAGEKEYLTWLERKDKGAPLNHVLREQLVQLRDEFNLDQYKFPWE

Specific function: Unknown

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787020, Length=345, Percent_Identity=32.463768115942, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786727, Length=323, Percent_Identity=30.030959752322, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1790000, Length=328, Percent_Identity=26.219512195122, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536633, Length=313, Percent_Identity=35.1437699680511, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17507179, Length=312, Percent_Identity=33.6538461538462, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24667904, Length=316, Percent_Identity=37.6582278481013, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45553211, Length=316, Percent_Identity=37.6582278481013, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24667912, Length=316, Percent_Identity=37.6582278481013, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24667908, Length=316, Percent_Identity=37.6582278481013, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24648360, Length=365, Percent_Identity=29.5890410958904, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24658010, Length=324, Percent_Identity=29.6296296296296, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 41268; Mature: 41268

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVVYDDVVWLDFDVLENFMVDVFKALGVPEEDAKIVANVLITADKQGIDSHGVGRLKPI
CCEEECCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCHH
YVERIQAGIQNPITNFEIVRESPTTAVVDGHNGMGHVIGYKAMKLAIEKAKKYGMGMVAV
HHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEE
RNSTHYGIAGYYVQMAVDEGMIGITGTNARPSIAPTFGVEPMLGTNPLTFGMPSDEEFPF
ECCCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCE
ILDCATSVSQRGKIEVYARAGKEIPEGWVIGEDGKTRTDTEQILEDLKKGKAALVPLGGI
EEEEHHHHCCCCCEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCC
GEETAGYKGYGYATVVEILSAALQGGNFLKMLTGFDEQGNRVPYRIGHFFIAINIEAFTE
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCCEEEEEEEEEEEHHHHHH
LENFKKTTGEILRALRNSKKAPGAERIYTAGEKEYLTWLERKDKGAPLNHVLREQLVQLR
HHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DEFNLDQYKFPWE
HHCCCCCCCCCCC
>Mature Secondary Structure
MEVVYDDVVWLDFDVLENFMVDVFKALGVPEEDAKIVANVLITADKQGIDSHGVGRLKPI
CCEEECCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCHH
YVERIQAGIQNPITNFEIVRESPTTAVVDGHNGMGHVIGYKAMKLAIEKAKKYGMGMVAV
HHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEE
RNSTHYGIAGYYVQMAVDEGMIGITGTNARPSIAPTFGVEPMLGTNPLTFGMPSDEEFPF
ECCCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCE
ILDCATSVSQRGKIEVYARAGKEIPEGWVIGEDGKTRTDTEQILEDLKKGKAALVPLGGI
EEEEHHHHCCCCCEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCC
GEETAGYKGYGYATVVEILSAALQGGNFLKMLTGFDEQGNRVPYRIGHFFIAINIEAFTE
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCCEEEEEEEEEEEHHHHHH
LENFKKTTGEILRALRNSKKAPGAERIYTAGEKEYLTWLERKDKGAPLNHVLREQLVQLR
HHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DEFNLDQYKFPWE
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]