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The map label for this gene is gacH [H]

Identifier: 239616812

GI number: 239616812

Start: 427127

End: 428086

Strand: Direct

Name: gacH [H]

Synonym: Kole_0405

Alternate gene names: 239616812

Gene position: 427127-428086 (Clockwise)

Preceding gene: 239616811

Following gene: 239616813

Centisome position: 18.55

GC content: 41.98

Gene sequence:

>960_bases
ATGCGTGCCCTGTACTTCGATGAGAAGCTTTCCATGGTCGAGGTTCCAAAACCATCTCCAGAAGATGGCGAAGCACTTAT
AAAGGTTTTAGCAGCAGGTATATGTAACACGGATATTGAGATAGTGAAAGGTTACATGGGATTCAAAGGCATTCCAGGTC
ACGAGTTCGTTGGAATTGTTGAAAAGTCCGCTGATCCACGTTGGGTGGGGAAACGTGTTGTCGGGGAGATCAATATAGCC
TGTAATGAGTGTGATCTGTGTAAAATAGGGTTGAAAAAACATTGTCGGAATATAAAGGTACTCGGTATAAAGGACTGGAA
TGGAGCCTTCGCTGAGTATCTTGTTCTCCCCGTTGAAAACCTTCACGAAGTCCCTTCGAAAGTTTCTAACCGCCAAGCAG
TTTTTGTAGAACCTTTAGCTGCCGCTATTCAGATACTGGAACAGGTCCACATCAAACCAACAGACAGAGTTTGTGTTATT
GGTGATGGTAAACTTGGCCTGTTGATTTCTCTTTCTTTGAACGCCCATGGTGTAAAACATATTCTTGTTGGCAAGCATCC
TGAAAAAATGAAGATAGTTTCTGACAGAGGCGTAAGTACATTGTTTCCTGGTGATGTTCAAAGTTTTGATAGATTTTTCG
ACGTTGTTATCGAGGCCACCGGGAATCAAGCCGGTTTGGAAACGGCTCTAAACATCATCAGACCCCAGGGAACAGTAGTA
TTGAAAAGCACTTATGCGTCAAAAGCTTCTGTTGATCTCTCTAAAGTTGTGGTTAATGAGATAAATATAATAGGTTCAAG
ATGTGGTCCATTTGAGCCAGCCTTACAACTTTTGGAGAAAGATTTGATTGACGTAAATCCCTTGGTGTCCCAGGTTCTTC
CCTTTGAAAAAGCTTTAGAAGCTTTTGAACTGGCGAAAACCAAAGGGACACTTAAAGTTATTCTAGAGGTGGAAAAATGA

Upstream 100 bases:

>100_bases
TTTGGATTCTCGAGAAAAACCGTGCGAATATCTACCGGATTGGAACACCCGGATGATCTTATAGAGGATATCGAGAGAGC
CCTTAAAGGAGTTGAGTGAT

Downstream 100 bases:

>100_bases
AATACGAAATAGTTTATTTCGACCTTGATGGTACCCTTCTCGATTTTGAGCGTTCAGAAGCTGAGGCTCTGACAGCACTC
ATGGCTAGCGGGGGATACTC

Product: Alcohol dehydrogenase GroES domain protein

Products: NA

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRALYFDEKLSMVEVPKPSPEDGEALIKVLAAGICNTDIEIVKGYMGFKGIPGHEFVGIVEKSADPRWVGKRVVGEINIA
CNECDLCKIGLKKHCRNIKVLGIKDWNGAFAEYLVLPVENLHEVPSKVSNRQAVFVEPLAAAIQILEQVHIKPTDRVCVI
GDGKLGLLISLSLNAHGVKHILVGKHPEKMKIVSDRGVSTLFPGDVQSFDRFFDVVIEATGNQAGLETALNIIRPQGTVV
LKSTYASKASVDLSKVVVNEINIIGSRCGPFEPALQLLEKDLIDVNPLVSQVLPFEKALEAFELAKTKGTLKVILEVEK

Sequences:

>Translated_319_residues
MRALYFDEKLSMVEVPKPSPEDGEALIKVLAAGICNTDIEIVKGYMGFKGIPGHEFVGIVEKSADPRWVGKRVVGEINIA
CNECDLCKIGLKKHCRNIKVLGIKDWNGAFAEYLVLPVENLHEVPSKVSNRQAVFVEPLAAAIQILEQVHIKPTDRVCVI
GDGKLGLLISLSLNAHGVKHILVGKHPEKMKIVSDRGVSTLFPGDVQSFDRFFDVVIEATGNQAGLETALNIIRPQGTVV
LKSTYASKASVDLSKVVVNEINIIGSRCGPFEPALQLLEKDLIDVNPLVSQVLPFEKALEAFELAKTKGTLKVILEVEK
>Mature_319_residues
MRALYFDEKLSMVEVPKPSPEDGEALIKVLAAGICNTDIEIVKGYMGFKGIPGHEFVGIVEKSADPRWVGKRVVGEINIA
CNECDLCKIGLKKHCRNIKVLGIKDWNGAFAEYLVLPVENLHEVPSKVSNRQAVFVEPLAAAIQILEQVHIKPTDRVCVI
GDGKLGLLISLSLNAHGVKHILVGKHPEKMKIVSDRGVSTLFPGDVQSFDRFFDVVIEATGNQAGLETALNIIRPQGTVV
LKSTYASKASVDLSKVVVNEINIIGSRCGPFEPALQLLEKDLIDVNPLVSQVLPFEKALEAFELAKTKGTLKVILEVEK

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=339, Percent_Identity=25.6637168141593, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1787863, Length=313, Percent_Identity=27.4760383386581, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI226510992, Length=306, Percent_Identity=29.4117647058824, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790045, Length=334, Percent_Identity=26.6467065868263, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788075, Length=343, Percent_Identity=26.530612244898, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790718, Length=303, Percent_Identity=23.7623762376238, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788407, Length=330, Percent_Identity=24.2424242424242, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788073, Length=319, Percent_Identity=22.257053291536, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17562876, Length=341, Percent_Identity=23.4604105571848, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562878, Length=334, Percent_Identity=24.5508982035928, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322619, Length=324, Percent_Identity=24.0740740740741, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319955, Length=324, Percent_Identity=24.0740740740741, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323099, Length=320, Percent_Identity=24.0625, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319258, Length=298, Percent_Identity=26.510067114094, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=304, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319257, Length=348, Percent_Identity=24.4252873563218, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45550770, Length=323, Percent_Identity=25.6965944272446, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI45551930, Length=323, Percent_Identity=25.6965944272446, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI221457811, Length=323, Percent_Identity=25.6965944272446, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17737897, Length=341, Percent_Identity=25.5131964809384, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17137530, Length=341, Percent_Identity=23.1671554252199, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR006140
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 34821; Mature: 34821

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALYFDEKLSMVEVPKPSPEDGEALIKVLAAGICNTDIEIVKGYMGFKGIPGHEFVGIV
CCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEE
EKSADPRWVGKRVVGEINIACNECDLCKIGLKKHCRNIKVLGIKDWNGAFAEYLVLPVEN
ECCCCCHHHHHHHEEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHEECCHHH
LHEVPSKVSNRQAVFVEPLAAAIQILEQVHIKPTDRVCVIGDGKLGLLISLSLNAHGVKH
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCEEE
ILVGKHPEKMKIVSDRGVSTLFPGDVQSFDRFFDVVIEATGNQAGLETALNIIRPQGTVV
EEECCCCCCEEEECCCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEE
LKSTYASKASVDLSKVVVNEINIIGSRCGPFEPALQLLEKDLIDVNPLVSQVLPFEKALE
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHHHH
AFELAKTKGTLKVILEVEK
HHHHHHCCCEEEEEEEECC
>Mature Secondary Structure
MRALYFDEKLSMVEVPKPSPEDGEALIKVLAAGICNTDIEIVKGYMGFKGIPGHEFVGIV
CCCEEECCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEE
EKSADPRWVGKRVVGEINIACNECDLCKIGLKKHCRNIKVLGIKDWNGAFAEYLVLPVEN
ECCCCCHHHHHHHEEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHEECCHHH
LHEVPSKVSNRQAVFVEPLAAAIQILEQVHIKPTDRVCVIGDGKLGLLISLSLNAHGVKH
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCEEE
ILVGKHPEKMKIVSDRGVSTLFPGDVQSFDRFFDVVIEATGNQAGLETALNIIRPQGTVV
EEECCCCCCEEEECCCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEE
LKSTYASKASVDLSKVVVNEINIIGSRCGPFEPALQLLEKDLIDVNPLVSQVLPFEKALE
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCHHHHHH
AFELAKTKGTLKVILEVEK
HHHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA