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The map label for this gene is mdeA [H]

Identifier: 239616811

GI number: 239616811

Start: 425938

End: 427125

Strand: Direct

Name: mdeA [H]

Synonym: Kole_0404

Alternate gene names: 239616811

Gene position: 425938-427125 (Clockwise)

Preceding gene: 239616806

Following gene: 239616812

Centisome position: 18.5

GC content: 41.58

Gene sequence:

>1188_bases
GTGGAAAATAAAGATCTTCATTTTGATACTCTTGCGATACATGCGGCTGAAAAAACCGATCAAAATCAAGCATTAAATCC
ACCTATATACATGACCTCGACGTTCACTTTTACGGACTTACAGCAAGCTGACGATACATTCAGCTTCAAACGAAAGGCAT
TTGTTTATACCAGAGGTGGGAATCCAACGATAAACCTTTTTGAAGCTAGAATGGCTGCTCTGGAATCCGGTGTTGATTCC
GTAGCCTTTGCTTCTGGTATGGCGGCTATAACTTCTGTTTTGTTGTCCTTTGTTAAAAATGGTGATGAAATCCTTGCGCA
CAGAAACCTCTATGGTTCAACTTTTGGGGCTACCGCGCATCTTTTTCCGAATTATGGAATAATTACACGCTATGTGAACA
TGTCAGACTTAAAACAAGTTGAAAAGAGTATCACCCCAAAAACAAAGGTGCTTTATCTTGAAACTCCAACAAATCCATCT
CTGGAGATAATTGATATTGCTGAGTTATCTAAAATCGCAAGAAATTATGGCATCAAAGTTGTTGTGGATAACACCTTTGC
AACACCGTACTTTCAGAAACCCCTCGAACTTGGGGCTGACGTTGTTGTTCATAGTGCAACAAAATATATTTCCGGTCATG
GGGACGTTGTTGCTGGTGTAGCAATTTCTAAAGATCAGGAGTACATTCACAGATTAAAGTTTGGCTATATGTGCGAATTG
GGAGGTGTTTTGAGCCCATTTAATGCCTGGCTCCTCTTAAGGGGATTAAAAACACTTTCATTAAGAATGAAACGGCATGA
GGAGAACGCTATGGCAATTGTGCGATATCTCAAAAATCATCCTCTCGTTGAAAAGATCATGTATCCCGGGTTAGAAGAAC
ATCCTGGCCATGAGCTGGCAAGAAAACAAATGACTGGATTTGGTGGTATTGTCAGCTTTGAACTCAAAGGTGACGTGGAA
ACGGCGAAGGCTTTTGTTCGCAACCTTAAGCTTATAAAACTTGCTGTTAGCCTCGGGGACGCTGAAACCCTCGTTGAAGT
TCCAGCTCTTATGACTCATAGGGATTATCCCGAAGAAAAACTGCACGAGTTTGGATTCTCGAGAAAAACCGTGCGAATAT
CTACCGGATTGGAACACCCGGATGATCTTATAGAGGATATCGAGAGAGCCCTTAAAGGAGTTGAGTGA

Upstream 100 bases:

>100_bases
TCTTTTTTGCCTTCTATGTGACTCTGTTAAAATTTCAGCACAAGTTCATTTGTAGGGATATAATTAAATAAACGTAGTCT
ATAAGGAGGGTCTGAAAATC

Downstream 100 bases:

>100_bases
TATGCGTGCCCTGTACTTCGATGAGAAGCTTTCCATGGTCGAGGTTCCAAAACCATCTCCAGAAGATGGCGAAGCACTTA
TAAAGGTTTTAGCAGCAGGT

Product: Cystathionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MENKDLHFDTLAIHAAEKTDQNQALNPPIYMTSTFTFTDLQQADDTFSFKRKAFVYTRGGNPTINLFEARMAALESGVDS
VAFASGMAAITSVLLSFVKNGDEILAHRNLYGSTFGATAHLFPNYGIITRYVNMSDLKQVEKSITPKTKVLYLETPTNPS
LEIIDIAELSKIARNYGIKVVVDNTFATPYFQKPLELGADVVVHSATKYISGHGDVVAGVAISKDQEYIHRLKFGYMCEL
GGVLSPFNAWLLLRGLKTLSLRMKRHEENAMAIVRYLKNHPLVEKIMYPGLEEHPGHELARKQMTGFGGIVSFELKGDVE
TAKAFVRNLKLIKLAVSLGDAETLVEVPALMTHRDYPEEKLHEFGFSRKTVRISTGLEHPDDLIEDIERALKGVE

Sequences:

>Translated_395_residues
MENKDLHFDTLAIHAAEKTDQNQALNPPIYMTSTFTFTDLQQADDTFSFKRKAFVYTRGGNPTINLFEARMAALESGVDS
VAFASGMAAITSVLLSFVKNGDEILAHRNLYGSTFGATAHLFPNYGIITRYVNMSDLKQVEKSITPKTKVLYLETPTNPS
LEIIDIAELSKIARNYGIKVVVDNTFATPYFQKPLELGADVVVHSATKYISGHGDVVAGVAISKDQEYIHRLKFGYMCEL
GGVLSPFNAWLLLRGLKTLSLRMKRHEENAMAIVRYLKNHPLVEKIMYPGLEEHPGHELARKQMTGFGGIVSFELKGDVE
TAKAFVRNLKLIKLAVSLGDAETLVEVPALMTHRDYPEEKLHEFGFSRKTVRISTGLEHPDDLIEDIERALKGVE
>Mature_395_residues
MENKDLHFDTLAIHAAEKTDQNQALNPPIYMTSTFTFTDLQQADDTFSFKRKAFVYTRGGNPTINLFEARMAALESGVDS
VAFASGMAAITSVLLSFVKNGDEILAHRNLYGSTFGATAHLFPNYGIITRYVNMSDLKQVEKSITPKTKVLYLETPTNPS
LEIIDIAELSKIARNYGIKVVVDNTFATPYFQKPLELGADVVVHSATKYISGHGDVVAGVAISKDQEYIHRLKFGYMCEL
GGVLSPFNAWLLLRGLKTLSLRMKRHEENAMAIVRYLKNHPLVEKIMYPGLEEHPGHELARKQMTGFGGIVSFELKGDVE
TAKAFVRNLKLIKLAVSLGDAETLVEVPALMTHRDYPEEKLHEFGFSRKTVRISTGLEHPDDLIEDIERALKGVE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=391, Percent_Identity=43.7340153452685, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI299473759, Length=391, Percent_Identity=40.6649616368286, Blast_Score=261, Evalue=5e-70,
Organism=Homo sapiens, GI25453487, Length=389, Percent_Identity=38.0462724935733, Blast_Score=231, Evalue=7e-61,
Organism=Escherichia coli, GI1790375, Length=384, Percent_Identity=39.5833333333333, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1789383, Length=375, Percent_Identity=27.7333333333333, Blast_Score=167, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17560056, Length=392, Percent_Identity=42.6020408163265, Blast_Score=306, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI17538055, Length=392, Percent_Identity=42.3469387755102, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI71980708, Length=394, Percent_Identity=36.2944162436548, Blast_Score=248, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6319307, Length=394, Percent_Identity=38.5786802030457, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=34.7126436781609, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321170, Length=332, Percent_Identity=33.1325301204819, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=409, Percent_Identity=32.0293398533007, Blast_Score=182, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321904, Length=401, Percent_Identity=29.925187032419, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6323557, Length=333, Percent_Identity=28.5285285285285, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322590, Length=260, Percent_Identity=28.4615384615385, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322970, Length=357, Percent_Identity=26.6106442577031, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=40.2564102564103, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 44041; Mature: 44041

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKDLHFDTLAIHAAEKTDQNQALNPPIYMTSTFTFTDLQQADDTFSFKRKAFVYTRGG
CCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEEEHHHHHCCCCHHCCEEEEEEECCC
NPTINLFEARMAALESGVDSVAFASGMAAITSVLLSFVKNGDEILAHRNLYGSTFGATAH
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEE
LFPNYGIITRYVNMSDLKQVEKSITPKTKVLYLETPTNPSLEIIDIAELSKIARNYGIKV
ECCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHCCEEE
VVDNTFATPYFQKPLELGADVVVHSATKYISGHGDVVAGVAISKDQEYIHRLKFGYMCEL
EEECCCCCCHHHCHHHCCCHHEEHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
GGVLSPFNAWLLLRGLKTLSLRMKRHEENAMAIVRYLKNHPLVEKIMYPGLEEHPGHELA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHH
RKQMTGFGGIVSFELKGDVETAKAFVRNLKLIKLAVSLGDAETLVEVPALMTHRDYPEEK
HHHHCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCHHH
LHEFGFSRKTVRISTGLEHPDDLIEDIERALKGVE
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENKDLHFDTLAIHAAEKTDQNQALNPPIYMTSTFTFTDLQQADDTFSFKRKAFVYTRGG
CCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEEEHHHHHCCCCHHCCEEEEEEECCC
NPTINLFEARMAALESGVDSVAFASGMAAITSVLLSFVKNGDEILAHRNLYGSTFGATAH
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEE
LFPNYGIITRYVNMSDLKQVEKSITPKTKVLYLETPTNPSLEIIDIAELSKIARNYGIKV
ECCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHCCEEE
VVDNTFATPYFQKPLELGADVVVHSATKYISGHGDVVAGVAISKDQEYIHRLKFGYMCEL
EEECCCCCCHHHCHHHCCCHHEEHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
GGVLSPFNAWLLLRGLKTLSLRMKRHEENAMAIVRYLKNHPLVEKIMYPGLEEHPGHELA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHH
RKQMTGFGGIVSFELKGDVETAKAFVRNLKLIKLAVSLGDAETLVEVPALMTHRDYPEEK
HHHHCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCHHH
LHEFGFSRKTVRISTGLEHPDDLIEDIERALKGVE
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]