Click here to switch to the map view.

The map label for this gene is dapA

Identifier: 239616516

GI number: 239616516

Start: 121592

End: 122476

Strand: Reverse

Name: dapA

Synonym: Kole_0106

Alternate gene names: 239616516

Gene position: 122476-121592 (Counterclockwise)

Preceding gene: 239616517

Following gene: 239616515

Centisome position: 5.32

GC content: 45.42

Gene sequence:

>885_bases
ATGTTCAGAGGTGCTGCAAGTGCAGTTATAACACCATTTAAAGGAAATGAAATTGATTATGATAGCTTCGATAAGTTTTT
AAAGTTTCAGCTTGATTCAGGTATAAACGGGCTTATAGTCCTTGGAACCACAGGTGAAGCTCCAACTGTAACCTCAGAAG
AACGAAGAGAAATTATAAGCTTCGCTGTCAAGAAAGTCGGCGGCAGAGTACCGGTGATAATAGGTACGGGAAGCAATTCC
ACAGCCCATAGCATAGAACTTAGTCAGGAAGCCTTTGAGCTAGGTGCAGACGGTGTCCTGGTCGTGACACCCTACTACAA
CAAGCCTACCCAGGAGGGGCTATACAGACATTATGAAGCCATAGCAAAGAGTGTTCCCGGTCACGTAATTATATATAACG
TTCCCGGAAGGACCGGTATCGACATAATCCCTGAGACTGTCCTTCGCTGTGCACAGATAGAGAACATCGTGGGTATCAAA
GAGGCATCTGGAAATCAGGCTCAGGTAGATGAACTGCTTAGATTAAAGGCAAAAGTTAGGCCCGAATTCAAAATCTGGTC
TGGAAACGATGATCAGGCTTTCCATCTAGTTTGCAGTGGTGGAGACGGTGTCATTTCCGTTTTGTCAAACGTAGCACCTT
CACAGACCGTTGAAATGATTGCAGCGGCTCTTGAGGGTGATCTGGAAAAGGCCAGGGAGATCCATCTAAAGTTATTCCCC
CTGATGAAGGCCCTCTTTATAGAAACCAATCCCATTCCTGTTAAATTTGCCACTCATCTTCTGGGATTTGGTTCTGAAAC
CCTTAGATTACCACTGACTGAGGCCAGTAAGCGAACCAGGGAAGTTGTCAAAAAGGCAATGCAGGATTGTGGGGTGCTCA
GATGA

Upstream 100 bases:

>100_bases
GGGGAAGAAAACTATTACAGGTTACTTTGCAGGCCAATGGTGGGGAACTTGTTGTAAGTTTCAAAGAAGGCAACATATAT
TTAGGAGGAGGTGTTGAAAG

Downstream 100 bases:

>100_bases
ATTACGGTCTTATAGGACACACTGGACAGATGGGAAAGGCCATTCAGGAGGTAATGACAGACTCAAGGCTAGTCTACACC
CGTGATGAAAACGGGCGTAA

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MFRGAASAVITPFKGNEIDYDSFDKFLKFQLDSGINGLIVLGTTGEAPTVTSEERREIISFAVKKVGGRVPVIIGTGSNS
TAHSIELSQEAFELGADGVLVVTPYYNKPTQEGLYRHYEAIAKSVPGHVIIYNVPGRTGIDIIPETVLRCAQIENIVGIK
EASGNQAQVDELLRLKAKVRPEFKIWSGNDDQAFHLVCSGGDGVISVLSNVAPSQTVEMIAAALEGDLEKAREIHLKLFP
LMKALFIETNPIPVKFATHLLGFGSETLRLPLTEASKRTREVVKKAMQDCGVLR

Sequences:

>Translated_294_residues
MFRGAASAVITPFKGNEIDYDSFDKFLKFQLDSGINGLIVLGTTGEAPTVTSEERREIISFAVKKVGGRVPVIIGTGSNS
TAHSIELSQEAFELGADGVLVVTPYYNKPTQEGLYRHYEAIAKSVPGHVIIYNVPGRTGIDIIPETVLRCAQIENIVGIK
EASGNQAQVDELLRLKAKVRPEFKIWSGNDDQAFHLVCSGGDGVISVLSNVAPSQTVEMIAAALEGDLEKAREIHLKLFP
LMKALFIETNPIPVKFATHLLGFGSETLRLPLTEASKRTREVVKKAMQDCGVLR
>Mature_294_residues
MFRGAASAVITPFKGNEIDYDSFDKFLKFQLDSGINGLIVLGTTGEAPTVTSEERREIISFAVKKVGGRVPVIIGTGSNS
TAHSIELSQEAFELGADGVLVVTPYYNKPTQEGLYRHYEAIAKSVPGHVIIYNVPGRTGIDIIPETVLRCAQIENIVGIK
EASGNQAQVDELLRLKAKVRPEFKIWSGNDDQAFHLVCSGGDGVISVLSNVAPSQTVEMIAAALEGDLEKAREIHLKLFP
LMKALFIETNPIPVKFATHLLGFGSETLRLPLTEASKRTREVVKKAMQDCGVLR

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=288, Percent_Identity=26.7361111111111, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI13540533, Length=236, Percent_Identity=27.5423728813559, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1788823, Length=296, Percent_Identity=43.5810810810811, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI87082415, Length=296, Percent_Identity=36.1486486486487, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786463, Length=251, Percent_Identity=35.4581673306773, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789620, Length=236, Percent_Identity=27.9661016949153, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_KOSOT (C5CHX9)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002939838.1
- ProteinModelPortal:   C5CHX9
- GeneID:   7967524
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0106
- ProtClustDB:   CLSK2814016
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31963; Mature: 31963

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 160-160 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRGAASAVITPFKGNEIDYDSFDKFLKFQLDSGINGLIVLGTTGEAPTVTSEERREIIS
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHH
FAVKKVGGRVPVIIGTGSNSTAHSIELSQEAFELGADGVLVVTPYYNKPTQEGLYRHYEA
HHHHHHCCCEEEEEECCCCCCEEEEEECHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
IAKSVPGHVIIYNVPGRTGIDIIPETVLRCAQIENIVGIKEASGNQAQVDELLRLKAKVR
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
PEFKIWSGNDDQAFHLVCSGGDGVISVLSNVAPSQTVEMIAAALEGDLEKAREIHLKLFP
CCEEEECCCCCCEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMKALFIETNPIPVKFATHLLGFGSETLRLPLTEASKRTREVVKKAMQDCGVLR
HHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFRGAASAVITPFKGNEIDYDSFDKFLKFQLDSGINGLIVLGTTGEAPTVTSEERREIIS
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHH
FAVKKVGGRVPVIIGTGSNSTAHSIELSQEAFELGADGVLVVTPYYNKPTQEGLYRHYEA
HHHHHHCCCEEEEEECCCCCCEEEEEECHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
IAKSVPGHVIIYNVPGRTGIDIIPETVLRCAQIENIVGIKEASGNQAQVDELLRLKAKVR
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
PEFKIWSGNDDQAFHLVCSGGDGVISVLSNVAPSQTVEMIAAALEGDLEKAREIHLKLFP
CCEEEECCCCCCEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMKALFIETNPIPVKFATHLLGFGSETLRLPLTEASKRTREVVKKAMQDCGVLR
HHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA