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The map label for this gene is sigF [H]

Identifier: 238924921

GI number: 238924921

Start: 2446431

End: 2447105

Strand: Reverse

Name: sigF [H]

Synonym: EUBREC_2572

Alternate gene names: 238924921

Gene position: 2447105-2446431 (Counterclockwise)

Preceding gene: 238924922

Following gene: 238924919

Centisome position: 70.94

GC content: 40.44

Gene sequence:

>675_bases
ATGAAGAGTGACAAAATCAGCAGACTTGTCACAGAAAATATAAACCTGATTTATTTAGTTATGAAACGCTTTAGAAACAG
AGGCGTTGACCGGGAGGATTTATTTCAGATAGGAGCAGTTGGGCTGACAAAGGCAGCGCAGCGATTTGATGAAAGTAAGG
GCTTTGCCTTTTCTACATACGCAGTACCGTTGATAATAGGGGAAATACAGCGCTTTCTACGTGATGACGGAATGGTGCAT
ATCAGTCGAAAAATACTGGATGATGCAAGAAAAATTGCAATTATCAGAGAAAAATACAGAATGAATGAAAATAGTGAAAT
ATCACTTGAAAAAATGGAAATTATTTCCGGTATGTCAAAACAGGATATAATAATTGCCATGGAAGCTGATAAAAATGCAT
CGTCAATAGAAAATGAAAGTGTGCTTGATTATTTGGACAATAAAATGAGCGCAGAAGCATCAGACAAGCCATCGGAGACA
GATAAAGTGATAAATCACATCGCAATGCAGCAGGCGTTTAACTCACTGGATGATAGCGAGCGCAGACTGATTATGCTCCG
CTATATGCAGGATAAGACACAGAAGCAGGTGGCAGAAGTGCTTGGACGCAACCAGGTGGCCATATCACGAATGGAGAAGA
ATACATTGGAGAAGCTTCGCAGGGCGATGATGTGA

Upstream 100 bases:

>100_bases
GATCAGGCATGGGTTTTTCATTCATGGAAGCATTTATGGACGCGCTGACAGTTACATCAGAACCGGGACACGGAACAACA
GTAAAGATGAAAAAAGTACT

Downstream 100 bases:

>100_bases
GTGGGAAGCAGTGGAGGTTTTGAGCTTCCCACGTGCAGGCTGAGAGGATGCCGGGGGGCATGTAGTATATTCTTCAGAAA
ATTATGAAGTCAGCCCCGAG

Product: RNA polymerase sigma-F factor

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKSDKISRLVTENINLIYLVMKRFRNRGVDREDLFQIGAVGLTKAAQRFDESKGFAFSTYAVPLIIGEIQRFLRDDGMVH
ISRKILDDARKIAIIREKYRMNENSEISLEKMEIISGMSKQDIIIAMEADKNASSIENESVLDYLDNKMSAEASDKPSET
DKVINHIAMQQAFNSLDDSERRLIMLRYMQDKTQKQVAEVLGRNQVAISRMEKNTLEKLRRAMM

Sequences:

>Translated_224_residues
MKSDKISRLVTENINLIYLVMKRFRNRGVDREDLFQIGAVGLTKAAQRFDESKGFAFSTYAVPLIIGEIQRFLRDDGMVH
ISRKILDDARKIAIIREKYRMNENSEISLEKMEIISGMSKQDIIIAMEADKNASSIENESVLDYLDNKMSAEASDKPSET
DKVINHIAMQQAFNSLDDSERRLIMLRYMQDKTQKQVAEVLGRNQVAISRMEKNTLEKLRRAMM
>Mature_224_residues
MKSDKISRLVTENINLIYLVMKRFRNRGVDREDLFQIGAVGLTKAAQRFDESKGFAFSTYAVPLIIGEIQRFLRDDGMVH
ISRKILDDARKIAIIREKYRMNENSEISLEKMEIISGMSKQDIIIAMEADKNASSIENESVLDYLDNKMSAEASDKPSET
DKVINHIAMQQAFNSLDDSERRLIMLRYMQDKTQKQVAEVLGRNQVAISRMEKNTLEKLRRAMM

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

None

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 25831; Mature: 25831

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDKISRLVTENINLIYLVMKRFRNRGVDREDLFQIGAVGLTKAAQRFDESKGFAFSTY
CCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
AVPLIIGEIQRFLRDDGMVHISRKILDDARKIAIIREKYRMNENSEISLEKMEIISGMSK
HHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
QDIIIAMEADKNASSIENESVLDYLDNKMSAEASDKPSETDKVINHIAMQQAFNSLDDSE
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
RRLIMLRYMQDKTQKQVAEVLGRNQVAISRMEKNTLEKLRRAMM
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSDKISRLVTENINLIYLVMKRFRNRGVDREDLFQIGAVGLTKAAQRFDESKGFAFSTY
CCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHH
AVPLIIGEIQRFLRDDGMVHISRKILDDARKIAIIREKYRMNENSEISLEKMEIISGMSK
HHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
QDIIIAMEADKNASSIENESVLDYLDNKMSAEASDKPSETDKVINHIAMQQAFNSLDDSE
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
RRLIMLRYMQDKTQKQVAEVLGRNQVAISRMEKNTLEKLRRAMM
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2513372 [H]